Preparation method of biomimetic cartilage doped ordered magnetic nano-short fibers spliced double-layer hydrogel

A magnetic nano and short fiber technology, applied in the field of biomedical engineering, can solve problems such as poor mechanical properties, achieve low cost, excellent compression resistance, and promote cell proliferation and differentiation

Inactive Publication Date: 2019-06-04
BEIHANG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0013] The purpose of the present invention is to overcome the disadvantages of poor mechanical properties of hydrogels, prepare double-layered hydrogels doped with ordered magnetic nano-short fibers, simulate natural cartilage tissue, have excellent compression resistance and fatigue resistance, and provide excellent support for cell growth. Provides a magnetic environment conducive to the repair and regeneration of cartilage tissue

Method used

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  • Preparation method of biomimetic cartilage doped ordered magnetic nano-short fibers spliced double-layer hydrogel
  • Preparation method of biomimetic cartilage doped ordered magnetic nano-short fibers spliced double-layer hydrogel

Examples

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Embodiment 1

[0038] Example 1: Preparation of poly(lactic-co-glycolic acid) / polyacrylamide / sodium alginate biomimetic cartilage doped with ordered magnetic nano-short fibers spliced ​​bilayer hydrogel

[0039] Prepare 24% (mass / volume) poly(lactic-co-glycolic acid) copolymer (molecular weight is 100,000) spinning solution, solvent is the mixed solution of N,N-dimethylformamide and dichloromethane (4 / 3, volume / volume ), mixed the ferroferric oxide magnetic particles with the spinning solution (magnetic particles: macromolecule = 1:5, mass: mass), stirred at room temperature for 5 hours, then ultrasonically dispersed for half an hour, and obtained magnetic nano Fiber membrane, where the solution flow rate is 0.2mL / h, the applied voltage is 12-14kV, and the needle-to-receiver distance is 12cm. Cut the magnetic nanofiber membrane into 1 × 1 cm 2of small pieces. Mix 1 g of cut short fiber film with ethanol solution, and stir for 5 minutes with a high-speed shear homogenizer with a rotating sp...

Embodiment 2

[0040] Example 2: Preparation of Polycaprolactone / Polyacrylamide Grafted Chitosan / Sodium Alginate Biomimetic Cartilage Doped with Ordered Magnetic Nano Short Fibers Splicing Bilayer Hydrogel

[0041] Prepare 14% (mass / volume) polycaprolactone spinning solution, solvent is the mixed solution (1 / 5, volume / volume) of methanol and chloroform, ferromagnetic particle is mixed with spinning solution (magnetic particle: Polymer=1:5, mass:mass), stirred at room temperature for 5 hours, then ultrasonically dispersed for half an hour, and obtained magnetic nanofibrous membrane by electrospinning technology, wherein the solution flow rate was 0.4mL / h, and the applied voltage was 15-19kV , the needle-to-receiver distance is 15 cm. Cut the magnetic nanofiber membrane into 1 × 1 cm 2 Mix 1 g of cut short fiber film with ethylene glycol solution, and stir for 5 minutes with a high-speed shear homogenizer with a rotation speed of 15,000 rpm, and the length of the short fiber obtained is less ...

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Abstract

The invention discloses a preparation method of a biomimetic cartilage doped ordered magnetic nano-short fibers spliced double-layer hydrogel. According to the invention, nano-fibers containing magnetic particles are prepared by an electrospinning technology, and the nano-fibers are cut by a high-speed shear type homogenizer to obtain short fibers; the short fibers are mixed with a gel precursor and a cross-linking agent, and under the action of an external magnetic field, the magnetic short fibers are arranged orderly; by UV-irradiation, the gel precursor is cross-linked to obtain hydrogel doped with the ordered magnetic short fibers; two pieces of hydrogel are spliced in the way that the short fibers are perpendicular to each other, and spliced hydrogel is obtained relying on magnetic nanoparticles in the two pieces of hydrogel attracting each other; the spliced hydrogel is immersed in a sodium alginate solution, and a calcium chloride solution is added for gelatinization of the sodium alginate solution so as to obtain spiced double-layer hydrogel. The spiced double-layer hydrogel has a similar structure to cartilage tissue, good mechanical properties and magnetic properties, canpromote cell directed proliferation and differentiation, and is used for cartilage tissue repair.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a method for preparing bionic cartilage-doped ordered magnetic nanofiber spliced ​​double-layer hydrogel. Background technique [0002] Cartilage has excellent compression resistance, fatigue resistance and lubrication properties, which are inseparable from its special structural properties. Cartilage is composed of a polysaccharide matrix and vertically oriented multilayered collagen fibers, and its anisotropic composite structure endows it with excellent properties. But cartilage has no blood vessels and cannot regenerate. At present, the clinical treatment of cartilage defects mainly relies on autologous or allogeneic transplantation, which faces problems such as less supply sources and easy induction of immune reactions. Based on the special structure and properties of cartilage, biomimetic hydrogels with similar structures can be prepared to obtain cartilage repair or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/28C08J3/24A61L27/52A61L27/50A61L27/40A61L27/02A61L27/18A61L27/16A61L27/20C08L67/04C08L33/26C08L5/04
Inventor 李昆李萍张英楠王靖熙樊瑜波
Owner BEIHANG UNIV
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