Method and sampling device for separation and purification of microbial strains in water body

A technology for microbial strains and collection devices, which is applied in biochemical equipment and methods, methods for sampling biological materials, and separation of microorganisms, etc. problems, to achieve the effect of in-situ sampling, separation and identification, sturdy periphery, and small equipment footprint

Active Publication Date: 2021-11-23
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the purification of microbial strains, the existing methods mainly include ten-fold dilution separation method, plate streaking method, plate coating method, puncture separation method, single cell separation method and ultramicro membrane separation method, etc., but these methods often The process is fine and the procedures are cumbersome, especially the operator is required to have a very high level of operation, which will not only greatly reduce the purification efficiency of pure microbial strains, but also cause the loss of the activity of some microbial strains

Method used

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  • Method and sampling device for separation and purification of microbial strains in water body
  • Method and sampling device for separation and purification of microbial strains in water body
  • Method and sampling device for separation and purification of microbial strains in water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Step 1, the NH of 1.0g / L 4 Cl, 0.3g / L of K 2 HPO 4 , 0.3g / L of KH 2 PO 4 , 0.1g / L KCl, 0.1g / L CaCl 2 2H 2 The NaCl of 0,30g / L, the yeast extract of 0.1g / L, the cysteine ​​of 0.5g / L, the sodium thiosulfate of 3.16g / L and 1ml trace element nutrient solution are mixed to obtain the culture medium solution of 1L, Wherein the trace element nutrient solution contains copper, manganese, zinc, iron, magnesium, molybdenum and boron, and the culture medium solution is adjusted to pH 7.6 with 10M KOH solution. In terms of the percentage of volume in milliliters, add 0.5% agar powder, heat and dissolve to prepare a solution;

[0057] Step 2, uniformly disperse polyvinyl alcohol fibers with a length of 10 mm in 5% NaOH solution, stir at 50° C. for 12 hours, collect the fibers and dry them;

[0058] Step 3, according to the ratio of the quality of the modified polyvinyl alcohol fiber silk to the mass of the BM solid medium solution, uniformly mix 1% of the modified polyvinyl a...

Embodiment 2

[0068] Step 1, the NH of 1.0g / L 4 Cl, 0.3g / L of K 2 HPO 4 , 0.3g / L of KH 2 PO 4 , 0.1g / L KCl, 0.1g / L CaCl 2 2H 2 The NaCl of 0,30g / L, the yeast extract of 0.1g / L, the cysteine ​​of 0.5g / L, the sodium thiosulfate of 3.16g / L and 1ml trace element nutrient solution are mixed to obtain the culture medium solution of 1L, Wherein the trace element nutrient solution contains copper, manganese, zinc, iron, magnesium, molybdenum and boron, and the culture medium solution is adjusted to be 7 with the KOH solution of 10M, and the culture medium solution after adjusting the pH according to the mass grams of agar powder In terms of the percentage of volume in milliliters, add 0.5% agar powder, heat and dissolve to prepare a solution;

[0069] Step 2, uniformly disperse polyvinyl alcohol fibers with a length of 5 mm in 5% NaOH solution, stir at 60° C. for 8 hours, collect the fibers and dry them;

[0070] Step 3, according to the ratio of the quality of the modified polyvinyl alcohol...

Embodiment 3

[0080] Step 1, the NH of 1.0g / L 4 Cl, 0.3g / L of K 2 HPO 4 , 0.3g / L of KH 2 PO 4 , 0.1g / L KCl, 0.1g / L CaCl 2 2H 2 The NaCl of 0,30g / L, the yeast extract of 0.1g / L, the cysteine ​​of 0.5g / L, the sodium thiosulfate of 3.16g / L and 1ml trace element nutrient solution are mixed to obtain the culture medium solution of 1L, Wherein the trace element nutrient solution contains copper, manganese, zinc, iron, magnesium, molybdenum and boron, and the KOH solution of 10M is used to adjust the culture medium solution to a pH of 7.3, and the culture medium solution after adjusting the pH according to the mass grams of agar powder In terms of the percentage of volume in milliliters, add 0.5% agar powder, heat and dissolve to prepare a solution;

[0081] Step 2, uniformly dispersing polyvinyl alcohol fibers with a length of 8mm in 5% NaOH solution, stirring at 55°C for 10h, collecting the fibers and drying them;

[0082] Step 3, according to the ratio of the quality of the modified poly...

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Abstract

A method and a sampling device for the separation and purification of microbial strains in a water body of the present invention, comprising step 1, adding the sample to be separated and purified and calcium carbonate solution into a sodium alginate solution to obtain a precipitate and a suspension; step 2, adding the sample to the lost Add suspension to oil phase A composed of sorbitan fatty acid ester and liquid paraffin to obtain mixed system A; step 3, add 6-10ml of glacial acetic acid to mixed system A, react at 22-25°C for 8- 12min to obtain the mixed system B, add calcium chloride solution to the mixed system B to obtain microcapsules, wash the microcapsules with sterilized water to obtain the microcapsule strain bank; step 4, inoculate the microcapsule strain bank into the porous plate one by one Cultivate a single colony, in which the BM liquid culture medium solution of the bacteria to be separated and purified is installed in the porous plate; the present invention combines the emulsified microsphere method and the porous plate method, and can be used as a library and a library for environmental functional microbial ecological germplasm resources. separate.

Description

technical field [0001] The invention relates to the field of separation and cultivation of water body microorganisms, in particular to a method for separation and purification of water body microorganism strains and a sampling device. Background technique [0002] The purification of microbial strains refers to the process of separating different types of microorganisms mixed together to obtain a single strain required for production and research. It is a key technology for the application and research of microbial industries and is widely used in microbial anti-corrosion, Industrial fields and experimental research fields such as water pollution control, mineral smelting, and functional bacterial agent development. In the specific purification, the corresponding culture medium is generally prepared according to the strains to be purified for separate isolation. In the purification of microbial strains, the existing methods mainly include ten-fold dilution separation method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/02C12M1/26
CPCC12M33/04C12N1/02
Inventor 朱超杨永林王慧琴马宏瑞贾柳陈喆倩
Owner SHAANXI UNIV OF SCI & TECH
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