Human hair quick lysate for trace drug detection and application of lysate

A lysing solution and hair technology, applied in the preparation of test samples, measuring devices, biological testing and other directions, can solve the problems of high protein impurity content, difficult to promote the grassroots level, low release efficiency, etc., and achieve high drug release rate and cracking speed. The effect of a rapid and accelerated release process

Active Publication Date: 2019-09-06
江苏苏博生物医学科技南京有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, the background of the acid hydrolysis hair product is low, but the release efficiency of the drug molecule of the test substance is lower than that of the alkaline hydrolysis method; the alkali hydrolysis can completely dissolve the hair, so that the drug molecule to be

Method used

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  • Human hair quick lysate for trace drug detection and application of lysate
  • Human hair quick lysate for trace drug detection and application of lysate
  • Human hair quick lysate for trace drug detection and application of lysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Detection of amphetamine-type drugs in the hair of drug-related personnel

[0036] According to the above lysate formula, the hair of drug-related personnel is processed and tested, and the operation steps are as follows:

[0037] Step 1. Hair sample collection: According to the "Standards for the Detection of Hair Samples of Drug-Related Personnel", start collecting from the root of the hair of the monitored person, mark the hair root and hair tail, and use hair 3cm away from the root, 30 hairs, about 5mg;

[0038] Step 2. Physically break the hair sample: use scissors to cut the collected hair sample into thin sections of 0.5mm-1mm to obtain hair sample powder;

[0039] Step 3. Preparation of mixed drug release solution in hair: absorb the lysate at a ratio of 500ul per 5mg of hair sample, put it in a 1.5ml centrifuge tube and mix it with the hair sample powder, and oscillate with ultrasound (frequency 25KHZ, power 300W) for 90 seconds to prepare Obtain dr...

Embodiment 2

[0042] Example 2: Detection of morphine-like drugs in the hair of drug-related personnel

[0043] Step 1. Hair sample collection: According to the "Standards for the Detection of Hair Samples of Drug-Related Personnel", start collecting from the root of the hair of the monitored person, mark the hair root and hair tail, and use hair 3cm away from the root, 30 hairs, about 5mg;

[0044] Step 2. Physically break the hair sample: use scissors to cut the collected hair sample into thin sections of 0.5mm-1mm to obtain hair sample powder;

[0045] Step 3. Preparation of the drug release mixed solution in hair: absorb the lysate at a ratio of 500ul per 5mg of hair sample, put it in a 1.5ml centrifuge tube, mix it with the hair sample powder, and oscillate with ultrasound for 90 seconds to prepare the drug release mixed solution in hair ;

[0046] Step 4: Take 5 drops (about 100uL) of the mixed drug release solution in hair prepared in step 3, drop it vertically into the sample hole ...

Embodiment 3

[0048] Example 3: Detection of ketamine-like drugs in the hair of drug-related personnel

[0049] Step 1. Hair sample collection: According to the "Standards for the Detection of Hair Samples of Drug-Related Personnel", start collecting from the root of the hair of the monitored person, mark the hair root and hair tail, and use hair 3cm away from the root, 30 hairs, about 5mg;

[0050] Step 2. Physically break the hair sample: use scissors to cut the collected hair sample into thin sections of 0.5mm-1mm to obtain hair sample powder;

[0051] Step 3. Preparation of the drug release mixed solution in hair: absorb the lysate at a ratio of 500ul per 5mg of hair sample, put it in a 1.5ml centrifuge tube, mix it with the hair sample powder, and oscillate with ultrasound for 90 seconds to prepare the drug release mixed solution in hair ;

[0052] Step 4: Take 5 drops (about 100uL) of the mixed drug release solution in hair prepared in step 3, and drop it vertically into the sample h...

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Abstract

The invention discloses a human hair quick lysate for trace drug detection and application of the lysate. The human hair quick lysate for trace drug detection comprises the raw material components of50-100nM of alkaline protease AH-101, 0.5-2% of mercaptoacetic acid, 25-75mM of glycine, 30-60mM of a trihydroxymethyl aminomethane hydrochloride buffer solution, 40-80mM of a sodium chloride-sodium hydroxide buffer solution and 0.5-1.5% of polyethylene glycol octylphenol ether (Triton X-100), wherein nM is nanomoles per liter, mM is millimoles per liter, and % is volume percent. Through the humanhair quick lysate, trace drug small molecules can be quickly extracted from a human hair sample, the drug release rate is high, the influence of impurities is small, and the lysate is free of toxinsand pollution; the lysate can be used under ordinary experimental conditions, keratin can be sufficiently cracked, cracking speed is high, entire cracking time is only 90 seconds, and the drug molecules are effectively released; and compared with a cracking method in the prior art, operation time is greatly shortened, and the truthfulness and reliability of an experiment result is guaranteed.

Description

technical field [0001] The invention relates to a rapid human hair lysate for trace drug detection and an application thereof, belonging to the field of drug detection in vivo. Background technique [0002] Drug abuse refers to the repeated and massive use of some drugs with dependence or dependence potential irrespective of the medical purpose. The drug users adopt the form of self-administration, leading to mental and physical dependence, causing mental confusion and some abnormal behavior. Substance abuse is also known as "drug use" or "drug abuse." Drug abuse is a major problem that seriously endangers social security and human life and health. According to statistics, the number of new-type drug users in my country increases by about 30% every year, of which young people account for 86%. In order to effectively combat drug crimes and cooperate with law enforcement departments such as public security in drug control work, researchers have established a series of accur...

Claims

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Application Information

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IPC IPC(8): G01N1/40G01N1/34G01N33/48
CPCG01N1/34G01N1/4044G01N33/48
Inventor 王宇峰苏雪峰刘洋春
Owner 江苏苏博生物医学科技南京有限公司
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