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Specific glycogen medium containing mixed syrup spice, preparation method and application thereof

A culture medium and syrup technology, applied in the field of microbial cultivation, can solve the problems of inability to accumulate in large quantities, low yield, low utilization rate of raw materials, etc., and achieve the effect of simple use and increased synthesis concentration

Inactive Publication Date: 2019-09-20
CATCH BIO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In nature, many microorganisms can convert sugars into acetoin during glycolysis to avoid acidosis. More researches mainly include Klebsiella, Baciiius, Enterobacter genus (Enterobacter), Serratia (Serratia), Lactococcus (Lactococcus), Hanseniaspora (Hanseniaspora) and Aeromonas (Aeromonas), etc., but the production capacity is quite different, and the raw material The utilization rate is low; at the same time, the production of acetoin is accompanied by a large number of organic acid by-products such as 2,3-butanediol, 2,3-butanedione, acetic acid, lactic acid, etc., the yield is generally low, and cannot be accumulated in large quantities

Method used

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  • Specific glycogen medium containing mixed syrup spice, preparation method and application thereof
  • Specific glycogen medium containing mixed syrup spice, preparation method and application thereof
  • Specific glycogen medium containing mixed syrup spice, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A specific glycogen culture medium containing mixed syrup spices, the culture medium includes the following components:

[0019]

[0020] Add water to 1000 mL, wherein the specific sugar source substance is a mixed syrup flavor, and the mixed syrup flavor includes rhamnose and xylose.

[0021] The preparation method is as follows:

[0022] Take 1kg of xylose mother liquor, the xylose mother liquor is the remaining processing waste liquid after high-temperature acid hydrolysis of corncobs and extraction of xylose, after testing, it contains 35.98% of xylose, 14.66% of arabinose, 5.23% of glucose, and 2.41% of galactose, adding 200g rhamnose, preheated at 90°C for 35 minutes, passed through a 0.45 micron membrane filter to obtain a mixed syrup spice, which contained 16.67% rhamnose, 29.98% xylose, and 12.22% arabinose %, glucose 4.34%, galactose 2.01%;

[0023] Take 100 g of mixed syrup spices, add 10 g of corn steep liquor, 5 g of yeast extract, 3 g of urea, 1 g of ...

Embodiment 2

[0026] A specific glycogen culture medium containing mixed syrup spices, the culture medium includes the following components:

[0027]

[0028] Add water to 1000 mL, wherein the specific sugar source substance is a mixed syrup flavor, and the mixed syrup flavor includes rhamnose and xylose.

[0029] The preparation method is as follows:

[0030] Take 10kg of fresh Dioscorea punctatus, grind and sieve, centrifuge for 15 minutes, add alcohol to extract the centrifuged precipitate twice, combine the extracts and concentrate, and the remaining residue is the crude saponin extract;

[0031] Dry the crude saponin extract at 60°C to constant weight, add an appropriate amount of acid to hydrolyze it for a certain period of time at 0.14MPa, and filter;

[0032] Add Ca(OH) to the filtrate 2 Neutralize to neutral or slightly acidic, filter again and concentrate the filtrate to a water content of about 50%, pass through a 0.45 micron filter membrane to sterilize and filter to obtain...

Embodiment 3

[0036] A specific glycogen culture medium containing mixed syrup spices, the culture medium includes the following components:

[0037]

[0038] Add water to 1000 mL, wherein the specific sugar source substance is a mixed syrup flavor, and the mixed syrup flavor includes rhamnose and xylose.

[0039] The preparation method is as follows:

[0040] 10kg of Acanthopanax, add 5 times of deionized water, and add 1.5% mixed enzyme at the same time, the mixed enzyme includes α-amylase and glucoamylase, α-amylase: glucoamylase=1:3, treat at 57°C for 90min , then treated in 3.6% NaOH solution at 75°C for 90 minutes, washed repeatedly with deionized water until neutral, added 1% cellulase and 10 times distilled water, adjusted the pH to 5.5, and placed it at 55°C for 2 hours;

[0041] Filtrate, add 4 times of ethanol to the filtrate, let stand for 1h, filter again, precipitate and dry, add 20 times of sulfuric acid with a concentration of 1mol / L, hydrolyze at 100°C for 6h, neutraliz...

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Abstract

The present application relates to the field of microbial culture, and concretely relates to a specific glycogen medium containing a mixed syrup spice, a preparation method and an application thereof. The medium comprises the following components: 80-120 g of specific carbohydrate source; 8-12 g of corn syrup; 4-6 g of a yeast extract; 2.4-3.6 g of urea; 0.8-1.2 g of dipotassium hydrogen phosphate; 0.4-0.6g of potassium chloride; and 0.08-0.12g of magnesium sulfate; wherein water is added to 1000mL, the specific sugar source material is the mixed syrup spice, and the mixed syrup spice comprises rhamnose and xylose. The specific glycogen medium containing the mixed syrup spice is simple to use, can effectively increase the synthetic concentration of acetoin and the conversion rate of a substrate, reduces the fermentation cost, and improves the economic benefit.

Description

technical field [0001] The invention relates to the field of microorganism culture, more specifically, it relates to a specific glycogen culture medium containing mixed syrup spices, a preparation method and application. Background technique [0002] Acetoin, chemical name 3-hydroxy-2-butanone (acetoin), also known as methyl acetylmethanol, 2,3-butanol ketone, is a natural substance with a special milky aroma, naturally occurring in corn, grapes , cocoa, apples, bananas, strawberries and some animal tissues, as an aroma compound, widely used in food, chemical, pharmaceutical and tobacco fields. In GB2760, it is listed as a synthetic spice for food that is allowed to be used. At the same time, acetoin is an important 4-carbon platform compound. In 2004, the US Department of Energy listed it as one of the 30 priority platform compounds for development and utilization. [0003] At present, the production of acetoin is mainly synthesized by chemical methods using diacetyl or 2,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26C12R1/125C12R1/865
CPCC12P7/26
Inventor 贾远敏
Owner CATCH BIO SCI & TECH