Gold nanoparticles activated by mercaptophenylboronic acid and preparation method and application of gold nanoparticles

A mercaptophenylboronic acid and gold nanoparticle technology, applied in bandages, filament/wire forming, fiber processing, etc., can solve the problems of nano-silver instability, unclear influence, complex composition, etc., and achieve excellent biocompatibility, The effect of avoiding secondary damage and simple preparation method

Active Publication Date: 2019-10-01
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art discloses the preparation method of silver-loaded bacterial cellulose hydrogel antibacterial dressing and its products. The bacterial cellulose hydrogel film is soaked in the silver metal precursor solution, and antibacterial is realized through multiple steps such as washing, dehydration, and sterilization. , but the preparation process of this method is complex and nano-silver is unstable, and has strong toxicity to the human body. The US FDA has clearly prohibited the use of nano-silver in medical treatment, and my country's CFDA has also restricted nano-silver in the medical field.
The prior art also discloses a nano-gold-containing antibacterial dressing and its preparation method. An effective antibacterial agent without drug resistance to bacteria is prepared by using ligand-modified gold

Method used

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  • Gold nanoparticles activated by mercaptophenylboronic acid and preparation method and application of gold nanoparticles
  • Gold nanoparticles activated by mercaptophenylboronic acid and preparation method and application of gold nanoparticles
  • Gold nanoparticles activated by mercaptophenylboronic acid and preparation method and application of gold nanoparticles

Examples

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Embodiment 1

[0076] The diagram of the device designed by the present invention is as follows: figure 1 shown. This example is used to illustrate the preparation method and performance characterization of p-mercaptophenylboronic acid-activated gold nanoparticles.

[0077] (1) In a round-bottomed flask, mix 0.05 mmol of p-mercaptophenylboronic acid (4MBA molecular weight, 153.99, Sigma), 0.05 mmol of chloroauric acid trihydrate (393.83 molecular weight, Sinopharm Chemical Reagent Co., Ltd.), 50 μl The triethylamine and 30 mg of Tween 80 were dissolved in 10 mL of methanol solution and mixed in an ice-water bath for 10 min until the molecules were completely dissolved.

[0078] (2) Dissolve 6 mg of sodium borohydride in 2 ml of methanol, add dropwise to the round-bottomed flask under vigorous stirring (1000 rpm), the color of the solution in the flask immediately turns brown, keep this reaction condition and react again 2 hours.

[0079] (3) The obtained p-mercaptophenylboronic acid-activ...

Embodiment 2

[0087] This example is used to illustrate the preparation method and performance characterization of m-mercaptophenylboronic acid-activated gold nanoparticles.

[0088] Proceed as follows:

[0089] (1) In a round-bottomed flask, mix 0.05 mmol of m-mercaptophenylboronic acid (3MBA molecular weight 153.99, Sigma), 0.05 mmol of chloroauric acid trihydrate (393.83 molecular weight, Sinopharm Chemical Reagent Co., Ltd.), 50 μl The triethylamine and 30 mg of Tween 80 were dissolved in 10 mL of methanol solution and mixed in an ice-water bath for 10 min until the molecules were completely dissolved.

[0090] (2) The synthesis of m-mercaptophenylboronic acid-modified gold nanoparticles is the same as that in Example 1. The results are shown in Figure 2B-C, with an average diameter of 1.8 nm and a particle size range of 1-5 nm.

[0091] (3) The antibacterial performance and antibacterial mechanism of the gold nanoparticles modified by m-mercaptophenylboronic acid are the same as in Ex...

Embodiment 3

[0095] Proceed as follows:

[0096] (1) The synthesis and characterization of p-mercaptophenylboronic acid-modified gold nanoparticles are the same as in Example 1.

[0097] (2) 10 mL of the obtained p-mercaptophenylboronic acid-activated gold nanoparticles were removed from the solution by a rotary evaporator (IKARV10, Germany) at 40 °C, and the solvent was redissolved into 5.0 mL of hexafluoroisopropanol (1,1, 1,2,2,2-Hexafluoro-2-propanol, HFIP, Sigma), and mixed with 1.0 g of polylactic acid-glycolic acid copolymer (PLGA, 50 / 50, Sigma) and 0.5 g of gelatin (gelatin, A Type powder, from pig skin, Sigma) was mixed evenly, stirred with a magnetic stirrer at room temperature for 2 hours, and prepared into a spinning precursor solution.

[0098] (3) The performance characterization of the antibacterial composite fiber membrane is the same as that of Example 1.

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Abstract

The invention provides gold nanoparticles activated by mercaptophenylboronic acid and a preparation method and application of the gold nanoparticles. The preparation method of the gold nanoparticles activated by the mercaptophenylboronic acid is simple, bacteria are prevented from being induced to generate drug resistance, and the antibacterial effect is significant; the gold nanoparticles have high biosecurity; an antibacterial fibrous membrane prepared through an electrostatic spinning method has stable physical and chemical performance and high porosity, can isolate pollutants, is soft andbreathable, has good wettability, can perfectly fit wounds of different shapes, is prepared from a degradable material and reduces the secondary damage brought by dressing replacement. The gold nanoparticles activated by the mercaptophenylboronic acid are stable and easy to store, and after the gold nanoparticles activated by the mercaptophenylboronic acid are prepared into a dressing through theelectrostatic spinning technology, high stability, the potential of large-scale production and a wide clinical application prospect are achieved.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a gold nanoparticle activated by mercaptophenylboronic acid, and a preparation method and application thereof. Background technique [0002] Bacterial infections, especially wound infections, are a serious threat to human health and even lead to death. Gram-positive bacteria are more likely to cause skin and cartilage infections than Gram-negative bacteria. In recent years, the number of patients dying from bacterial infections has increased dramatically, a phenomenon that is inseparable from the misuse of antibiotics. With the abuse of antibiotics, serious drug resistance has been induced in bacteria, leading to the superbug crisis. Therefore, it is very urgent to seek effective new antibacterial drugs, especially for multidrug-resistant superbugs. Due to the large specific surface area, high degree of surface functionalization, and unique physical and chemical...

Claims

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Application Information

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IPC IPC(8): A61L15/46A61L15/20A61L15/18A61L15/42A61L15/32A61L15/26D04H1/728D01D5/00
CPCA61L15/46A61L15/20A61L15/18A61L15/42A61L15/425A61L15/325A61L15/32A61L15/26D04H1/728D01D5/003D01D5/0069D01D5/0076D01D5/0092A61L2300/102A61L2300/404C08L67/04C08L89/00Y02A50/30
Inventor 蒋兴宇王乐
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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