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Compound bacterial agent containing bacillus velezensis and application thereof in preparation of bacteriostatic agent for aquatic bacteria

A technology of Bacillus Velez and composite bacterial agent, which is applied in the field of preparation of antibacterial agents for aquatic bacteria, can solve the problems of less research on Bacillus Velez, and achieve good application prospects and environmental friendly effects

Active Publication Date: 2019-11-19
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few relevant researches on Bacillus Veles in the field of aquatic products

Method used

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  • Compound bacterial agent containing bacillus velezensis and application thereof in preparation of bacteriostatic agent for aquatic bacteria
  • Compound bacterial agent containing bacillus velezensis and application thereof in preparation of bacteriostatic agent for aquatic bacteria
  • Compound bacterial agent containing bacillus velezensis and application thereof in preparation of bacteriostatic agent for aquatic bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation and Identification of Bacillus Velez YFI-4

[0024] 1. Isolation of strains

[0025] Culture pond water samples were collected from the culture area. Use 0.85% sterile normal saline to dilute the water samples from the culture pond continuously 10 times for 6 times, use a pipette gun to draw 100 μL of the solution in each concentration gradient dilution solution onto the BHI solid plate, and spread it evenly with a coating rod. Number and do 3 repetitions. After coating evenly, place it in the ultra-clean workbench for 5-10 minutes, so that the bacterial liquid on the surface of the medium is fully absorbed. Finally, the plate was inverted and incubated in a constant temperature incubator at 30°C for 24h. Colonies of different forms were selected and inoculated on ordinary broth plates for isolation and purification.

[0026] 2. Strain screening

[0027] The antibacterial properties of the isolated and purified bacteria were measured by the Oxford cup pla...

Embodiment 2

[0043] Antibacterial test of Bacillus Velez YFI-4 and its application in aquaculture ponds:

[0044] 1) Antibacterial spectrum experiment:

[0045] Bacillus Velez YFI-4 and Aeromonas veronii CCTCC AB98045, Aeromonas hydrophila ATCC 13040, Pseudomonas fluorescens CCTCC AB 92001, Ruby Yersinia ruckeri ATCC 29473, Elizabethkingia meningoseptica ATCC 13253, Streptococcus iniae ATCC 29177, Edwardsiella tarda ATCC 15947, Pseudomonas putida ATCC12633), Streptococcus agalactiae (Streptococcus agalactiae ATCC 12386), Citrobacter freundii ATCC43864, Plesiomons shigelloides ATCC 14029, Aeromonas schubertii ATCC43700, Nutella Cardiac bacteria (Nocardia seriolae ATCC43993) were respectively inoculated into liquid medium, cultured with shaking at 200 rpm at 30°C for 24 hours, and resuspended with 0.85% sterile saline for counting, so that the final concentration of Bacillus velei YFI-4 and pathogenic bacteria liquid were both 1×10 6 CFU / mL. Pipette 100 μl of pathogenic bacteria liquid a...

Embodiment 3

[0069] Preparation of compound bacterial agent:

[0070] The composite bacterial agent of this implementation is made by Bacillus velei YFI-4, Bacillus subtilis, and Bacillus licheniformis respectively through primary seed and secondary seed cultivation, fermentation culture, centrifugal concentration and drying, and compounding. The proportion of effective viable bacteria of Bacillus stifii YFI-4, Bacillus subtilis and Bacillus licheniformis is 3:1:1, and the effective bacterial concentration of the compound bacterial agent made is 10 10 CFU / g.

[0071] Both the Bacillus subtilis and the Bacillus licheniformis were purchased from Beijing Suolaibao Technology Co., Ltd.

[0072] The medium used in this implementation is LB broth, and the formula is as follows: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000ml, and adjust the pH to 7.4±0.2.

[0073] The drying method used in this implementation is: pulverize the bran, zeolite powder and corn flour, an...

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Abstract

The invention belongs to the fields of development and application of microorganisms, and in particular, relates to a compound bacterial agent containing bacillus velezensis and an application thereofin preparation of drugs for aquatic animal diseases. The compound bacterial agent includes aeromonas veronii, aeromonas hydrophila, pseudomonas fluorescens, yersinia ruckeri, elizabethkingia meningoseptica and pseudomonas putida. The compound bacterial agent provided by the invention has no pathogenicity to aquatic animals, and has good inhibitory effect on aquatic pathogenic bacteria including aeromonas veronii, aeromonas hydrophila, pseudomonas fluorescens, yersinia ruckeri, elizabethkingia meningoseptica and pseudomonas putida. The compound bacterial agent has a good control effect on bacterial diseases of aquatic animals, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of development and application of microorganisms, and in particular relates to a composite bacterial agent containing Bacillus Veles and its application in preparing an aquatic bacteria bacteriostatic agent. [0002] technical background [0003] China is the world's largest producer and consumer of aquatic products. With the intensive farming, the deterioration of the aquatic ecological environment has become increasingly prominent, and diseases are on the verge of occurrence. According to statistics, the annual loss due to diseases in my country reaches 15 billion yuan. In the process of aquaculture, Aeromonas victoria and Aeromonas hydrophila can cause outbreaks of bacterial diseases in a variety of farmed animals and cause huge losses to the culture. At present, drugs are still the most important means to control the occurrence of diseases in fishery production. Its raw materials are mainly transplanted pesticides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A61K35/742A61P31/00C12R1/125C12R1/10C12R1/07
CPCA61K35/742A61P31/04C02F3/34C12N1/20C12N1/205C12R2001/07A61K2300/00
Inventor 薛明洋周勇范玉顶孟彦刘文枝江南李逸群曾令兵
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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