Bacterium-enzyme synergistic fermentation method containing camellia seed meal

A camellia seed and starter technology, applied in animal feed, animal husbandry, applications, etc., can solve problems such as interference with nutrient absorption, reduced protein digestibility, strong toxicity, etc., to reduce bitterness, bitterness, and astringency. The effect of moderate acid production

Pending Publication Date: 2019-12-20
JIANGNAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

At present, feed processing plants generally use acidifiers to achieve the optimum pH for livestock, ignoring the importance of palatability to livestock. In addition, it is currently impossible to effectively deal with polyphenols such as tannins and flavonoids in camellia seed meal , resulting in reduced protein digestibility and interference with the absorption of certain nutrients (such as Fe, Ca, etc.)
Some large machinery manufacturers have tr

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  • Bacterium-enzyme synergistic fermentation method containing camellia seed meal
  • Bacterium-enzyme synergistic fermentation method containing camellia seed meal
  • Bacterium-enzyme synergistic fermentation method containing camellia seed meal

Examples

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Example Embodiment

[0031] Preparation of indicator bacteria liquid: Inoculate the three indicator bacteria of Escherichia coli, Salmonella and Staphylococcus aureus in LB liquid medium and cultivate at 37°C for 24 hours.

[0032] Using the Oxford cup method: take a plate with a diameter of about 90 mm, pour 15-20 mL of heated and melted nutrient agar medium, and spread it evenly on the plate, and place it on a water platform to solidify as the bottom layer. Take another semi-solid nutrient agar medium (agar content is 1%) after heating and melting in an appropriate amount, then let it cool to 48-50℃, add 0.1-0.2mL of the indicator bacteria suspension to each 50-100mL medium, and place it on each plate Add 5mL to the medium to spread evenly on the bottom layer as a bacterial layer. Place 4-5 Oxford cups at equal distances on each plate for spare use. Each double-layer plate in the Oxford cup is dripped with 200μL of lactic acid bacteria supernatant. After incubating at 37°C for 10-13h, measure each ...

Example Embodiment

[0037] Example 1: Screening of strains

[0038] The strain of the present invention adopts a plate dilution separation method to isolate and screen a Gram-positive strain with good antibacterial effect from a camellia seed meal sample. The separation and screening methods are as follows:

[0039] 1. Dilution of mixed strains: Weigh a sample of camellia seed meal, put 1g of camellia seed meal into MRS medium at 37°C for 24 hours, and perform gradient dilution of the bacterial solution;

[0040] 2. Prepare MRS medium: peptone 10.0g, beef extract 8.0g, yeast powder 4.0g, glucose 20.0g, dipotassium hydrogen phosphate 2.0g, triammonium citrate 2.0g, sodium acetate 5.0g, magnesium sulfate heptahydrate 0.58g , 0.25g manganese sulfate tetrahydrate, 801mL Tween, 1L distilled water, sterilized at 115°C for 20 minutes;

[0041] 3. Preliminary screening of strains: 100 microliters of the bacterial solution after the gradient dilution in step 1 was applied to the MRS solid medium plate with bromo...

Example Embodiment

[0048] Example 2: Process determination and antibacterial effect of Lactobacillus plantarum fermented camellia seed meal

[0049] In order to explore the optimal nutrient component distribution ratio and fermentation process for the antibacterial activity of bacteria enzymes after synergistic fermentation, based on MRS medium, orthogonal experiments were designed to study the effects of the following four components on fermented camellia seed meal: water, cellulose Enzyme, alkaline protease, bacterial solution DY6. The factor levels are shown in Table 2.

[0050] Table 2 Screening factors and levels of bacteria enzyme synergistic fermentation components

[0051]

[0052] Table 3 Orthogonal experiment results

[0053]

[0054] Through the analysis of orthogonal experiment (see Table 3), the optimal process conditions for the fermentation of camellia seed meal with bacterial enzymes are: water content is 50%, cellulase is 300 U / g substrate, alkaline protease is 800 U / g substrate, The ...

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Abstract

The invention discloses a bacterium-enzyme synergistic fermentation method containing camellia seed meal, and belongs to the technical field of fermentation engineering. Lactobacillus plantarum DY6, alkaline protease and cellulase are used as leavening agents, the camellia seed meal is used as a substrate, the yield of organic acid and flavor substances of the camellia seed meal after the bacterium-enzyme fermentation is improved, the palatability is increased, and the synergistic fermentation method can be used for preparing feed additives. The lactobacillus plantarum can also inhibit the growth of harmful bacteria such as escherichia coli, staphylococcus aureus and salmonella in intestinal tracts of livestock and poultry, and is beneficial to intestinal health. Through the method disclosed by the invention, the added value of the camellia seed meal is further improved, and the recycling of waste can be conveniently realized.

Description

technical field [0001] The invention relates to a bacterial-enzyme synergistic fermentation method containing camellia seed dregs, belonging to the technical field of fermentation engineering. Background technique [0002] Lactic acid bacteria (LAB) are classified in the Lactobacillus family. Lactic acid bacteria are Gram-positive, non-spore-forming (except for individual genera), immobile or less motile, acid-tolerant cocci or bacilli that utilize fermentable sugars to produce large amounts of lactic acid. Lactic acid bacteria widely exist in human and animal intestines and in many foods. Lactic acid bacteria can not only enhance the nutritional value of food, improve the flavor of food, and help improve the preservation of food, but also regulate the normal flora of the human gastrointestinal tract and maintain micro-ecological balance, which is beneficial to the health of both humans and animals. By fermenting carbohydrates, lactic acid bacteria can secrete a large amou...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/14
CPCA23K10/12A23K10/14
Inventor 毛银邓禹卫亚峰李国辉赵运英周胜虎
Owner JIANGNAN UNIV
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