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Lactobacillus reuteri JYLB-131 for increasing vitality of alcohol dehydrogenase of people after drinking, and application of lactobacillus reuteri JYLB-131 to foods and drugs

A technology of JYLB-131 and Lactobacillus reuteri, which is applied to the functions, applications, and food ingredients of food ingredients, can solve the problems of expensive hangover drugs, lack of liver protection, and incontinence. To prevent lipid peroxidation and the harm of certain chemical substances, help the distribution of wine, and relieve discomfort symptoms

Inactive Publication Date: 2019-12-24
WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims to solve the above-mentioned problems such as headache, dizziness, drop in blood pressure, rapid heartbeat, incontinence of urine and urine, slow effect of commonly used anti-alcoholic methods, high price of anti-alcoholic medicines, and lack of liver-protecting effect after drinking alcohol. A kind of Lactobacillus reuteri JYLB-131, this bacterial classification has very strong antioxidation performance, good antibacterial effect, has higher ADH enzyme and superoxide dismutase activity simultaneously, reduces the activity of malondialdehyde It can effectively relieve problems such as headache, dizziness, blood pressure drop, rapid heartbeat, and incontinence after drinking alcohol. The commonly used hangover methods are slow to take effect, and the hangover drugs are expensive, and they can't protect the liver.

Method used

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  • Lactobacillus reuteri JYLB-131 for increasing vitality of alcohol dehydrogenase of people after drinking, and application of lactobacillus reuteri JYLB-131 to foods and drugs
  • Lactobacillus reuteri JYLB-131 for increasing vitality of alcohol dehydrogenase of people after drinking, and application of lactobacillus reuteri JYLB-131 to foods and drugs
  • Lactobacillus reuteri JYLB-131 for increasing vitality of alcohol dehydrogenase of people after drinking, and application of lactobacillus reuteri JYLB-131 to foods and drugs

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] The preparation method of the medium: add peptone, beef powder, yeast powder, K 2 HPO 4 ·7H 2 O, triammonium citrate, sodium acetate 3H 2 O, glucose, Tween 80, MgSO4 7H 2 O, MnSO4 4H 2 O, mix evenly, adjust the pH value to 6.2-6.5, subpackage, sterilize at 115°C for 15 minutes, and prepare MRS liquid medium;

[0051] The preparation of described plate medium: add peptone 10g, beef powder 5g, yeast powder 4g, K in 1000mL distilled water 2 HPO 4 ·7H 2 O 2g, triammonium citrate 2g, sodium acetate 3H 2 O 5g, glucose 20g, Tween 80 1mL, MgSO4 7H 2 O0.2g, MnSO4 4H 2 O 0.05g, mix evenly, add 15g of agar, adjust the pH value to 6.2~6.5, subpackage, the temperature is 115 ℃, and sterilize for 15 minutes to prepare the plate culture medium;

[0052] MRS liquid medium Ⅰ: add beef extract 10g, yeast extract 5g, peptone 10g, anhydrous sodium acetate 5g, calcium carbonate 1g, Tween-80 1mL, K in 1000mL distilled water 2 HPO 4 2g, 20g grapes, 5mL salt solution A, mix well, ...

Embodiment 1

[0055] A kind of Lactobacillus reuteri JYLB-131 provided by the invention

[0056] 1. The source of the strain

[0057] The sample came from the feces of a 30-year-old healthy male in Dali, Sichuan (adult male with no family genetic disease history);

[0058] 2. Isolation and purification of strains

[0059] 1) Dilute the sample in 10 gradients under sterile conditions, add deionized water to the sample, mix well, and prepare the bacterial solution;

[0060] 2) Inoculate 100 μL of the bacterial solution on the plate medium, and incubate overnight under anaerobic conditions at 37°C until the characteristic colonies with a diameter of 1.5 mm to 3 mm, a smooth surface, milky white, and regular surroundings grow out;

[0061] 3) Pick a characteristic colony with a diameter of 3 mm, inoculate it on a new plate medium, and anaerobically activate it at 37°C for 1 day to obtain a culture strain named JYLB-131.

[0062] 3. Identification of strains

[0063] 1) Select general primer...

Embodiment 2

[0068] Antioxidative properties of Lactobacillus reuteri JYLB-131

[0069] 1) Strain selection

[0070] Selected strains: Lactobacillus reuteri JYLB-131, ATCC53608, ATCC55148, ATCC55739, ATCC23272, DSMZ 8535, JCM 2764, DSMZ 20056, DSMZ 20053;

[0071] 2) Preparation of detection bacteria solution

[0072] Taking Lactobacillus reuteri JYLB-131 as an example to prepare Lactobacillus reuteri JYLB-131 for different culture time to be tested

[0073] ①The inoculation amount is calculated according to the volume ratio of 1%. Inoculate 1% Lactobacillus reuteri JYLB-131 into the MRS liquid medium, and cultivate it for 30 hours at 37°C. 1 hour and 30 hours of fermentation broth, and then the fermentation broth cultivated for different periods of time was centrifuged for 20 minutes at a speed of 3500 rpm, and the supernatant was taken respectively, and the pH value was adjusted to 6.0 to obtain 12 hours of cultivation. Lactobacillus reuteri JYLB-131 detection bacteria liquid, Lactoba...

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Abstract

The invention relates to a microorganism strain and application thereof, in particular to lactobacillus reuteri JYLB-131 for increasing vitality of alcohol dehydrogenase of people after drinking, andan application of the lactobacillus reuteri JYLB-131 to foods and drugs. The preservation number of the lactobacillus reuteri is CGMCC NO.18040, and the lactobacillus reuteri is preserved in the ChinaGeneral Microbiological Culture Collection Center. The lactobacillus reuteri JYLB-131 can increase antioxidant activity of organisms, eliminate free radicals, adjust endocrine, restrain propagation and colonization of harmful bacteria of intestinal tracts, reduce production of endotoxin, adjust balance of intestinal floras, alleviate liver cell damage, increase disease resistance, and have the effects of increasing the vitality of the alcohol dehydrogenase of people after drinking, reducing propanedial content of people after drinking, increasing the activity of superoxide dismutase of peopleafter drinking, and increasing the content of reduced glutathione content of people after drinking, so that discomfort of people after drinking is relieved and improved, and the problems of headache,vomiting, dizziness, fall of blood pressure, cardioacceleration and incontinence of faeces and urine of people after drinking can be solved.

Description

technical field [0001] The invention relates to a microbial strain and its application, in particular to a Lactobacillus reuteri JYLB-131 which improves the alcohol dehydrogenase activity after drinking and its application in food and medicine. Background technique [0002] Lactobacillus reuteri is a lactic acid bacterium present in the gut of all vertebrates and mammals. It has a strong adhesion ability, which can improve the distribution of intestinal flora, antagonize the colonization of harmful bacteria, and avoid intestinal diseases; Lactobacillus reuteri can produce a non-toxic substance called "Reuterin" Protein broad-spectrum antibacterial substances can extensively inhibit the growth of Gram-positive bacteria, Gram-negative bacteria, yeast, fungi and pathogens. Lactobacillus reuteri probiotic preparation can improve human body function, enhance immunity and promote human health. [0003] Alcohol is one of the indispensable drinks in people's life. Alcohol contains...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P39/02A61P1/16C12R1/225
CPCA23V2002/00A61K35/747A23L33/135A61P1/16A61P39/02C12N1/20C12N1/205C12R2001/225A23V2200/334
Inventor 潘玉林曹维超石娜娜马明磊文传军司书锋闫洪伟王维新孙川周文浩李翠华步敏王良修张丽萍刘联军赵兴元
Owner WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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