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Collagen-based natural polymer hydrogel and preparation method thereof

A hydrogel and collagen technology, applied in the preparation of sugar derivatives, chemical instruments and methods, food ingredients, etc., can solve the problems of easy separation, cross-linking of hydroxyapatite and collagen, etc., and achieve easy adhesion, excellent biological Compatibility, beneficial to growth and differentiation

Active Publication Date: 2020-02-04
无锡贝迪生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are reports in the prior art that combine collagen and hydroxyapatite to prepare cartilage materials, but the prior art mainly involves simple physical mixing of the two, which cannot intersect hydroxyapatite and collagen. In the subsequent use process, due to the different density and the form of existence in the body, the two will be easily separated, and there will be certain risks in the application in the body. Therefore, it is necessary to find a hydrogel that overcomes the long-term coexistence of the two. Glue system

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  • Collagen-based natural polymer hydrogel and preparation method thereof
  • Collagen-based natural polymer hydrogel and preparation method thereof
  • Collagen-based natural polymer hydrogel and preparation method thereof

Examples

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Embodiment 1

[0052] Oxidized guanidinoglycogen and its preparation method (the schematic diagram of the synthetic route of oxidized guanidinoglycogen is shown in figure 2 ):

[0053] (1) Positive electrification of glycogen: Weigh 1 g of glycogen and dissolve it in 70 mL of anhydrous dimethyl sulfoxide in a three-necked flask. After it is completely dissolved, add 1 g of N,N-carbonyldiimidazole, and activate it under nitrogen protection for 1 After that, dissolve 1.54ml of ethylenediamine in 10ml of dimethyl sulfoxide, slowly drop it into the reaction with a syringe, react for 24 hours under the protection of nitrogen, and dialyze the product in a 3500Da dialysis bag for 48h, and obtain amino sugar Original;

[0054] (2) Grafting guanidine group on aminoglycogen: Weigh 537.5mg guanidinoacetic acid, dissolve it in 50ml deionized water, adjust the pH to 5.5 with 1mol / L hydrochloric acid or sodium hydroxide, add 1.32g to the solution Carbodiimide hydrochloride and 792.5mg N-hydroxysuccinim...

Embodiment 2

[0063] A preparation of collagen-based natural polymer hydrogel, comprising the following steps:

[0064] 1) Preparation of oxidized guanidine glycogen: same as Example 1;

[0065] 2) Preparation of collagen solution: add 11.25mg of collagen to every 1mL of water, stir evenly at 37°C to obtain collagen solution;

[0066] 3) Preparation of oxidized guanidinoglycogen solution doped with nano-hydroxyapatite: add 37.5 mg oxidized guanidinoglycogen per 1 mL of water, stir well to obtain oxidized guanidinoglycogen solution, and then add 0 mg nano-hydroxyphosphorus Limestone, obtain the oxidized guanidine glycogen solution doped with nano-hydroxyapatite (0mg);

[0067] 4) Preparation of hydrogel: by volume, take 2 parts of collagen solution, 1 part of oxidized guanidine glycogen solution doped with nano-hydroxyapatite, and mix the oxidized guanidinium glycogen solution doped with nano-hydroxyapatite Pour the collagen solution under electric stirring (300rpm, the same below), mix ev...

Embodiment 3

[0069] A preparation of collagen-based natural polymer hydrogel and its induction of MSCs differentiation, comprising the following steps:

[0070] 1) Preparation of oxidized guanidine glycogen: same as Example 1;

[0071] 2) Preparation of collagen solution: Add 15mg of collagen to every 1mL of water, stir evenly at 37°C to obtain collagen solution;

[0072] 3) Preparation of oxidized guanidinoglycogen solution doped with nano-hydroxyapatite: add 50 mg oxidized guanidinoglycogen per 1 mL of water, stir evenly to obtain oxidized guanidinoglycogen solution, and then add 0 mg nano-hydroxyapatite to it Stone, obtain the oxidized guanidinoglycogen solution of doping nano-hydroxyapatite (0mg);

[0073] 4) Preparation of hydrogel by volume, take 2 parts of collagen solution, 1 part of oxidized guanidine glycogen solution doped with nano-hydroxyapatite, put the oxidized guanidinium glycogen solution doped with nano-hydroxyapatite in Pour the collagen solution under electric stirrin...

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Abstract

The invention discloses a collagen-based natural polymer hydrogel and a preparation method thereof, belonging to the hydrogel field. At first, a glycogen derivative is synthesized as a crosslinking agent, oxidize guanidine glycogen, the glycogen derivative has a plurality of groups on the surface, can connect the inorganic component hydroxyapatite and the organic component collagen through chemical bonds and electrostatic actions, and can better bond and combine the natural polymer material and the inorganic material, thus forming a hydrogel capable of effectively directionally inducing MSCs differentiation. The collagen-based natural polymer hydrogel prepared by the invention has certain stretchability, elasticity and bendability, and the compression modulus and the elastic modulus are within the interval range that can enable bone marrow mesenchymal stem cells to differentiate into cartilage and osteogenesis, and can ensure that it is not easy to be damaged when facing large bone damage.

Description

technical field [0001] The invention relates to a collagen-based natural polymer hydrogel and a preparation method thereof, belonging to the field of hydrogels. Background technique [0002] People in modern society have a fast pace of life and are used to walking fast, which can easily lead to bone and cartilage damage. Due to the lack of lymphatic, blood vessel and nerve distribution in bone tissue, the lack of nutrient supply during the repair process, only relying on the limited nutrition of the matrix and synovial fluid of the joint cavity and the weak self-division ability of osteoblasts and chondrocytes, the bone tissue cannot be effectively repaired. self-healing. Cartilage damage can cause clinical symptoms such as knee joint pain, joint instability, and joint stiffness. In severe cases, secondary osteoarthritis will occur, which is also an important cause of limb dysfunction and disability, and will seriously affect the quality of life of patients. Therefore, the...

Claims

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Application Information

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IPC IPC(8): C08B37/00C08L89/00C08L5/00C08K3/32C08J3/075C07H13/12C07H1/00A61L27/52A61L27/58A61L27/46A23L33/10A61P19/00
CPCA23V2002/00A61L27/46A61L27/52A61L27/58A61L2430/02A23L33/10A61P19/00C07H1/00C07H13/12C08B37/0006C08J3/075C08J2389/00C08J2405/00C08K3/32C08K2201/011A23V2200/30
Inventor 陈敬华任伟业张羲和周娟程咏梅陆金婷
Owner 无锡贝迪生物工程股份有限公司