LAMP synchronous detection method for staphylococcus aureus and salmonella in liquid milk and kit
A technology for Staphylococcus and Salmonella, applied in biochemical equipment and methods, microbial measurement/inspection, recombinant DNA technology, etc., can solve the problems that the freshest products cannot be put on the market and delivered to consumers, so as to prevent proliferation and Contamination, high sensitivity, and easy operation
Pending Publication Date: 2020-03-31
JILIN BUSINESS & TECH COLLEGE
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Abstract
The invention relates to a LAMP synchronous detection method for staphylococcus aureus and salmonella in liquid milk and a kit, which belong to the technical field of food safety rapid detection. Themethod comprises the following steps: extracting genomic DNA of the liquid milk on site, designing a primer group on the basis of specific molecular marker characteristics of genomes of staphylococcusaureus and salmonella, and synchronously detecting polluted staphylococcus aureus and salmonella in the liquid milk by adopting a loop-mediated isothermal amplification technology. The whole detection process has low requirements on environment and equipment, and whether a to-be-detected sample contains staphylococcus aureus or salmonella or not is determined by adding a color developing agent toobserve the color change in the tube. The method has the advantages of high sensitivity, good specificity, safety, harmlessness, no organic reagent and no nucleic acid binding dye, all reagents can be directly released into the environment, and the method has important significance for improving the quality safety of dairy foods and improving the food-borne pathogenic bacteria analysis and detection technology.
Application Domain
Microbiological testing/measurementAgainst vector-borne diseases +1
Technology Topic
genomic DNABiotechnology +14
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