P53 messenger RNA nanoparticles, preparation method thereof and application thereof in preparing drug for treating tumors

A technology of tumor drugs and nanoparticles, applied in DNA preparation, anti-tumor drugs, gene therapy, etc., can solve the problems of ineffective small molecule inhibitors, genome mutations, etc., and achieve the effect of enhancing chemotherapy sensitivity
CN110974804AInactive Publication Date: 2020-04-10HANGZHOU NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU NORMAL UNIVERSITY
Publication Date
2020-04-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The p53 messenger RNA nanoparticles, a preparation method thereof and an application thereof in preparing a drug for treating tumors belong to the technical field of genetic engineering. The p53 messenger RNA nanoparticles are prepared by the following method: 1, chemically modified p53mRNA is synthesized in vitro; and 2, a nano-system is prepared by a self-assembly method to deliver the p53 messenger RNA in vivo. The p53 messenger RNA nanoparticles play an anti-tumor and chemosensitivity-enhancing therapeutic role by restoring the anti-tumor function of p53. The advantages of the invention are as follows: 1, the p53 messenger RNA in the invention is successfully delivered to the p53 inactivated liver cancer and lung cancer tumor cells through the nano-system to effectively and rapidly induce cell apoptosis and G1 phase cell cycle arrest in vivo and in vitro, thus significantly inhibiting the growth of tumor cells; and 2, the p53 messenger RNA delivered by the nanoparticles can enhancethe anti-tumor effect of a mTOR inhibitor everolimus by supplementing the tumor suppressor p53 deleted in the tumor.
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Description

Technical field

[0001] The invention belongs to the technical field of genetic engineering, and specifically relates to the technical field of p53 messenger RNA nanoparticle and its preparation method and application in the preparation of tumor drugs. Background technique

[0002] In addition to the inactivation of tumor suppressor factors, the occurrence and development of cancer is also accompanied by abnormal activation of tumor growth-promoting functional pathways, such as the mammalian target of rapamycin (mTOR), a serine / threonine kinase that can promote cells Growth and proliferation. The mTOR signaling pathway is abnormally activated in 60% of tumors to increase the proliferation of tumor cells, including liver cancer and lung cancer.

[0003] Everolimus (RAD001) is a typical mTOR inhibitor and has been clinically approved by the FDA for use in advanced kidney cancer, pancreatic neuroendocrine tumors and other types of cancer. However, everolimus cannot effectively improv...

Claims

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