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Anti-rheumatoid arthritis human umbilical cord mesenchymal stem cell injection and preparation method thereof

A stromal stem cell and rheumatoid technology, which is applied in the field of human umbilical cord mesenchymal stem cell injection against rheumatoid arthritis and its preparation field, can solve the problem of difficult control of umbilical cord mesenchymal stem cell activity and rheumatoid arthritis. The treatment effect is uneven, affecting the curative effect and other problems, to achieve the effect of inhibiting the degree of cartilage destruction and bone erosion, reducing the degree of infiltration, and preventing the release

Pending Publication Date: 2020-08-07
海南和泽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing umbilical cord mesenchymal stem cells have been widely used in a large number of animal models and clinical studies, but the therapeutic effects on rheumatoid arthritis are uneven. The key lies in the difficulty of controlling the activity of umbilical cord mesenchymal stem cells. The mesenchymal stem cells in the preservation medium of the injection are prone to adhesion, aggregation, and poor stability, which affects the curative effect of the existing human umbilical cord mesenchymal stem cell injection in anti-rheumatoid arthritis

Method used

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  • Anti-rheumatoid arthritis human umbilical cord mesenchymal stem cell injection and preparation method thereof
  • Anti-rheumatoid arthritis human umbilical cord mesenchymal stem cell injection and preparation method thereof
  • Anti-rheumatoid arthritis human umbilical cord mesenchymal stem cell injection and preparation method thereof

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Effect test

Embodiment 1

[0025] Embodiment 1-An anti-rheumatoid arthritis human umbilical cord mesenchymal stem cell injection, each 100ml umbilical cord mesenchymal stem cell injection includes: human umbilical cord mesenchymal stem cell 6×10 5 1, human serum albumin 4g, yam polysaccharide 30mg, compound amino acid 90mg, sodium chloride 0.6g, sodium gluconate 0.6g, sodium acetate 0.4g, potassium chloride 0.04g and magnesium chloride 0.04g.

[0026] The compound amino acids include L-leucine, L-glutamic acid, L-arginine, L-proline, L-tyrosine, L-cysteine, glycine, L-phenylalanine , a mixture of L-tryptophan, L-threonine and L-lysine acetate;

[0027] The human umbilical cord mesenchymal stem cells are P4 generation human umbilical cord mesenchymal stem cells.

Embodiment 2

[0028] Example 2 - a human umbilical cord mesenchymal stem cell injection against rheumatoid arthritis, each 100ml of umbilical cord mesenchymal stem cell injection includes: human umbilical cord mesenchymal stem cells 4 × 10 5 1, human serum albumin 2g, yam polysaccharide 10mg, compound amino acid 60mg, sodium chloride 0.5g, sodium gluconate 0.5g, sodium acetate 0.3g, potassium chloride 0.03g and magnesium chloride 0.03g;

[0029] The compound amino acid comprises L-leucine, L-isoleucine, L-glutamic acid, L-arginine, L-proline, L-aspartic acid, L-cysteine, A blend of L-alanine, L-phenylalanine, L-tryptophan, L-lysine acetate and L-serine;

[0030] The human umbilical cord mesenchymal stem cells are P6 generation human umbilical cord mesenchymal stem cells.

Embodiment 3

[0031] Embodiment 3 - a preparation method of anti-rheumatoid arthritis human umbilical cord mesenchymal stem cell injection, comprising the following steps:

[0032] (1) Reagent materials: 5×10 human umbilical cord mesenchymal stem cells 5 1, human serum albumin 3g, yam polysaccharide 20mg, compound amino acid 70mg, sodium chloride 0.526g, sodium gluconate 0.502g, sodium acetate 0.368g, potassium chloride 0.037g and magnesium chloride 0.03g;

[0033] Wherein, the compound amino acid comprises: L-leucine, L-isoleucine, L-glutamic acid, L-arginine, L-proline, L-tyrosine, L-aspartic acid acid, L-cysteine, glycine, L-alanine, L-phenylalanine, L-tryptophan, L-threonine, L-lysine acetate and L-serine;

[0034] The human umbilical cord mesenchymal stem cells are P5 generation human umbilical cord mesenchymal stem cells;

[0035] The extraction method of the yam polysaccharide is to add pure water to the rhizome powder of yam according to the ratio of solid to liquid at 1:15, and t...

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Abstract

The invention provides an anti-rheumatoid arthritis human umbilical cord mesenchymal stem cell injection and a preparation method thereof. Every 100ml of the umbilical cord mesenchymal stem cell injection comprises: 4-6*10<5> human umbilical cord mesenchymal stem cells, 2-4g of human serum albumin, 10-30mg of Chinese yam polysaccharide, 60-90mg of compound amino acid, 0.5-0.6g of sodium chloride,0.5-0.6g of sodium gluconate, 0.3-0.4g of sodium acetate, 0.03-0.04g of potassium chloride and 0.03-0.04g of magnesium chloride. The human umbilical cord mesenchymal stem cell injection special for resisting rheumatoid arthritis prepared by the invention can effectively maintain the activity of umbilical cord mesenchymal stem cells, has good stability, also can effectively stop inflammatory mediator release, alleviates inflammatory cell infiltration degree, significantly reduces pannus number, and has good repair effect on rheumatoid arthritis bone and cartilage damage, thus having broad application prospects.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an anti-rheumatoid arthritis human umbilical cord mesenchymal stem cell injection and a preparation method thereof. Background technique [0002] Current clinical studies have shown that mesenchymal stem cells can not only reduce the incidence of arthritis and reduce the severity of experimental arthritis through immune regulation, but also repair the bone and cartilage damage that occurs in rheumatoid arthritis, which indicates that It has broad prospects for the treatment of rheumatoid arthritis. Since the successful isolation of mesenchymal stem cells from human umbilical cord Wharton's jelly in 2003, the umbilical cord has become another biological tissue rich in mesenchymal stem cells after bone marrow and fat. It has the advantages of low immunogenicity and low immunogenicity, and has the conditions for industrialization and clinical use. The existing umbilical cord m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/10A61K47/42A61K47/36A61K47/18A61P29/00A61P19/02
CPCA61K35/28A61K9/10A61K9/0019A61K47/42A61K47/36A61K47/183A61P29/00A61P19/02
Inventor 黄佩龙秦秀兵
Owner 海南和泽生物科技有限公司
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