Gene chip for identifying seven fungal infection pathogens as well as kit and detection method thereof

Abstract

The invention discloses a gene chip for identifying seven fungal infection pathogens. The gene chip comprises a solid phase carrier and a gene chip probe fixed on the solid phase carrier, wherein thegene chip probe comprises a to-be-detected sample oligonucleotide probe; wherein the to-be-detected sample oligonucleotide probe comprises a candida albicans probe, a candida glabrata probe, a candidaparapsilosis probe, a candida tropicalis probe, a candida krusei probe, an aspergillus fumigatus probe and an aspergillus flavus virus probe, and the probe sequences are sequentially shown as SEQ IDNO: 1-7. According to the invention, the gene chip for identifying seven human fungal infection pathogens is successfully constructed; each screening probe adopted by the gene chip prepared by the invention can be specifically combined with a target gene of a corresponding pathogen, a hybridization signal is relatively strong and stable, and the gene chip has a relatively great application prospect.

Description

technical field [0001] The present invention relates to the field of gene chip technology, and more specifically, relates to a gene chip for identification of seven kinds of fungal infection pathogens, a kit and a detection method thereof. Background technique [0002] Fungi are part of the normal flora of the human body, and exist in small amounts in the human mouth, upper respiratory tract, intestinal tract, urinary tract, and skin surface. Invasive fungal disease (IFD) refers to when the host's immune function is low or defective, due to opportunistic pathogenic fungi invade the human body, grow and multiply in tissues, organs or blood, and cause inflammation and tissue damage disease. The morbidity and mortality of IFD are on the rise in patients with hematological diseases, malignant tumors, organ transplantation, and long-term use of broad-spectrum antibiotics, glucocorticoids, and immunosuppressants. [0003] The main pathogenic fungi of invasive fungal infection in...

AI Technical Summary

Problems solved by technology

It is suitable for the early diagnosis of all deep fungal infections except cryptococcus and zygomycetes, especially candida and fungi, but cannot determine the species

Many false positives are found in clinical applications, such as: use of cellulose membrane for hemodialysis specimens or exposure of patients to gauze or other materials containing dextran, intravenous infusion of immunoglobulin, albumin, coagulation factors or blood products, streptococcemia or contamination by the operator handling the specimen

In addition, the use of polysaccharide anticancer drugs, mucosal damage caused by radiotherapy and chemotherapy, resulting in dextran in food or colonized Candida entering the blood through the gastrointestinal tract may also cause false positives

(3) Molecular biology techniques: including in situ hybridization, polymerase chain reaction, determination of G+C mol% content in DNA, random amplified polymorphic DNA (RAPD) analysis, restriction endonuclease polymorphism analysis (RFLP), etc., but the above-mentioned technologies are time-consuming and labor-intensive, do not have the characteristics of high-throughput, and cannot quickly detect infected fungi at the same time with a small amount of samples, so they are mostly limited to laboratory research and cannot be really applied to clinical practice.

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