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Lignocellulose material efficient decomposition composite bacterial system and culture method thereof

A technology of lignocellulose and culturing method, which is applied in the field of efficient decomposition of lignocellulose materials and the field of culturing compound bacteria, can solve the problems of low decomposition efficiency of straw, and achieve the effects of improving flexibility and improving decomposition efficiency.

Active Publication Date: 2020-10-30
山佳经典(北京)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a compound bacteria system that efficiently decomposes straw to solve the above-mentioned technical problem of low efficiency of straw decomposition, and at the same time provide a method for cultivating composite bacteria that efficiently decomposes straw, which balances the catalytic efficiency of enzymes and the enzyme production of bacteria efficiency

Method used

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  • Lignocellulose material efficient decomposition composite bacterial system and culture method thereof
  • Lignocellulose material efficient decomposition composite bacterial system and culture method thereof
  • Lignocellulose material efficient decomposition composite bacterial system and culture method thereof

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Experimental program
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Effect test

Embodiment 1

[0055] A complex bacterial system for efficiently decomposing lignocellulosic materials, which includes Bacillus subtilis that secretes amylase and protease, Bacillus cereus that secretes protease, Pseudomonas aeruginosa that secretes ligninase, and fiber that secretes The clostridium papyrilyticum of suzyme, the Pichia pastoris that secretes hemicellulase, the colony forming unit number ratio of each strain of described composite bacteria is: Bacillus subtilis: Bacillus cereus: Pseudomonas aeruginosa: lysate Paper clostridium: Pichia pastoris=12-15:13-15:15-18:23-25:24-26, the concentration of enzyme in the composite strain is 5g / L;

[0056] The optimum growth temperature of the constituent microorganisms of the complex flora is between 30-37°C, while the optimum temperature for the secreted lignocellulose-decomposing enzymes to function is between 50-60°C, and each The strains are matched to secrete a corresponding amount of enzymes, so that various enzymes can synergistical...

Embodiment 2

[0058] like Figure 1-2 As shown, the fermentation method of lignocellulosic materials efficiently decomposing complex bacterial strains:

[0059] Strain culture:

[0060] The seed liquid culture medium: peptone 12g, yeast extract powder 7g, NaCl 10g, potassium dihydrogen phosphate 0.42g, dipotassium hydrogen phosphate 0.21g, agar 15g, distilled water to 1L, pH 7.0, sterilized at 121°C for 15min. After the sterilization is finished, pour it on a flat plate, cool and solidify for later use.

[0061] Firstly, the preserved composite bacteria system is activated on a slant to restore its best performance and prepare for making seeds. Then, the slant-activated seeds were cultured by streaking on the ultra-clean bench, and placed in a 32°C incubator for 3 days. The seeds cultivated to the logarithmic growth phase were transferred into a 1L Erlenmeyer flask under a sterile environment, and cultured at 32° C. and 200 rpm for 2 days.

[0062] Seed liquid expansion culture:

[006...

Embodiment 3

[0067] like Figure 3-4 As shown, the decomposition of bagasse by the composite bacterial system:

[0068] experimental method:

[0069] The method in Example 1 was used to cultivate complex strains to produce enzymes, and a 2t fermenter was used for three-stage fermentation to produce enzymes, and then 10% corn stalks were added to the produced enzyme solution for 48 hours of enzymatic hydrolysis. The total weight loss and the weight loss of each component of the bagasse decomposed by the composite bacteria were measured at 0, 24 and 48 hours during the decomposition process.

[0070] Experimental results:

[0071] Changes of Decomposition Rate in the Decomposition Process of Bagasse by Composite Bacteria

[0072] At the beginning of enzymatic hydrolysis (0h), the decomposition rate of bagasse was 0%, the decomposition rate at 24h was 74%, and at the end of enzymolysis (48h), the decomposition rate of bagasse reached 90% . It can be seen that the composite bacteria syste...

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Abstract

The invention provides an efficient and rapid decomposition composite bacterial system and a culture method thereof. The optimum growth temperature of microorganisms is between 30 DEG C and 37 DEG C;the optimum temperature at which secreted related enzymes decomposed by lignocellulose play a role is between 50 DEG C and 60 DEG C; through temperature regulation, enzyme is enriched and produced atthe optimal growth temperature of microorganisms, and then the flexibility of the enzyme is improved after low-temperature activation, so that the lignocellulolytic enzyme is combined with a substrateat the lowest energy consumption and plays a catalytic role, and finally, at the high-temperature stage, the enzyme plays a maximum role at the high temperature, so that the lignocellulosic raw material is efficiently and quickly decomposed.

Description

technical field [0001] The invention relates to the field of straw decomposition, in particular to a compound bacterial strain for efficiently decomposing lignocellulosic materials and a cultivation method thereof. Background technique [0002] As a large agricultural country in my country, it is inevitable to produce a large number of crop straws. According to statistics, my country's annual straw production can reach 900 million tons, and the processing by-products are about 580 million tons. However, its comprehensive utilization rate is less than 40%, and the remaining 60% is randomly discarded, stacked or burned, which undoubtedly has an impact on the environment that cannot be ignored. Therefore, the development of new technologies to convert crop straw into high value-added products is of great significance to promote the sustainable development of social economy and the improvement of ecological environment. The composition of straw is lignocellulose, sugar, starch,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/125C12R1/085C12R1/385C12R1/84C12R1/145
CPCC12N1/20C12N1/16Y02W30/40
Inventor 温博婷崔宗均郭铭男
Owner 山佳经典(北京)科技有限公司
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