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Ferroferric oxide nanoparticles as well as preparation method and application thereof

A technology of ferroferric oxide and nanoparticles, which is applied in the field of inorganic nanomaterials, can solve the problems of unusable enzymes, high cost, and high cost of biosensors, and achieve the effect of avoiding precipitation reactions

Active Publication Date: 2021-02-05
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Economical: The cost of biosensors is relatively high, and once the enzyme falls in large quantities, it cannot be used again, cannot be reused, and the cost is relatively high

Method used

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  • Ferroferric oxide nanoparticles as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Embodiment 1: the preparation of ferric oxide nanoparticles

[0089] (1) Take 3.2g FeCl 3 ·6H 2 O, 1.5g FeSO 4 ·7H 2 O was dissolved in a mixed solvent of 10 mL of absolute ethanol and 10 g of glycerin, and 5 g of anhydrous sodium acetate was added as a co-solvent, 25 Hz ultrasonic for 5 min, and stirred at 40 ° C to completely dissolve the solid to obtain solution A;

[0090] (2) Take 10mL 0.0058mol / mL sodium dodecylbenzenesulfonate solution and 5mL n-butanol and mix evenly to obtain a mixing aid, then take 10mL toluene and add it to the mixing aid and mix to obtain a mixture, then drop while stirring Add 3mL of step (1) to obtain solution A, then add 3mL of 4mol / L NaOH solution dropwise while stirring, and stir until the solution becomes transparent and clear to obtain 31mL of solution B;

[0091] (3) Add 0.02g sodium polymethacrylate (purchased from Guangdong Wengjiang Chemical Reagent Co., Ltd., product number: PB063162, CAS number: 54193-36-1) to solution B, th...

Embodiment 2

[0094] Embodiment 2: the preparation of enzyme biosensor

[0095] Preparation of ferric oxide-β-glucosidase:

[0096] (I) Get the iron ferric oxide nanoparticles obtained in 0.1g Example 1, 0.2g PEG4000 (purchased from Shanghai Kaibite Chemical Co., Ltd.), 0.1g FeSO 4 and 0.1g K 2 S 2 o 8 Mix, then add 20mL of ethanol with a concentration of 50% (v / v), then add 20mL of water, stir until completely dissolved, keep the temperature at 25°C, then add 4mL of methyl acrylate for copolymerization reaction for 30min, to obtain a polymerization product;

[0097] (II) washing the polymerized product with ethyl acetate and distilled water subsequently, and then separating it with a magnet to obtain 0.2 g of magnetic particles;

[0098] (Ⅲ) Add 40 mL of 2% (volume fraction) hydrazine hydrate ethanol solution to the magnetic particles (take 2 mL of 80% (v / v) hydrazine hydrate solution, and use 50% (v / v) ethanol solution Dilute the volume to 100mL to obtain), react for 2h, place in a 6...

Embodiment 3

[0107] Before the experiment, the mass of the β-glucosidase on the nanometer ferroferric oxide coupled enzyme microsphere and the self-assembled β-glucosidase electrode was 0.225g; The mass of iron is 0.18g;

[0108] (1) Use the enzyme biosensor prepared in Example 2 to measure the concentration of glucose solution (5mol / L). When in use, adjust the external power supply voltage (i.e. external power supply voltage) to 1v, disconnect or close the switch every 2s, when The display was stable (4.0mol / L), and the applied voltage was adjusted to be 0.5v. After 15s, a total of 100mL of glucose solution was taken out, and the β-glucosidase content in the glucose solution was quantitatively determined by the Bradford method (the Bradford protein concentration assay kit was purchased from Shanghai Biyuntian Biotechnology Co., Ltd., product number P0006; the specific operation steps are carried out according to the instructions), the result: the protease concentration is 2.25μg / mL, that ...

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Abstract

The invention discloses ferroferric oxide nanoparticles as well as a preparation method and application thereof, which belong to the field of inorganic nano materials. The preparation method of the ferroferric oxide nanoparticles comprises the following steps: (1) dissolving ferrite and ferric salt in an organic solvent, adding a cosolvent, carrying out ultrasonic treatment, and stirring to obtaina solution A; (2) uniformly mixing a surfactant and a cosurfactant, adding toluene, adding the solution A, adding a NaOH solution, and stirring until the solution becomes transparent and clear to obtain a solution B; (3) adding a dispersing agent and an alkaline additive into the solution B to adjust the pH value, and stirring to obtain a microemulsion; and (4) adding NaHCO3 into the microemulsion, carrying out aging treatment, centrifuging, carrying out suction filtration, washing, separating, and drying to obtain the ferroferric oxide nanoparticles. According to the invention, enzyme is immobilized by adopting a covalent coupling method, so that directional movement of an external magnetic field can be realized, enzyme pollution is avoided, and the biological activity of the enzyme is not changed.

Description

technical field [0001] The invention belongs to the field of inorganic nanometer materials, and in particular relates to a ferric oxide nanoparticle and a preparation method and application thereof. Background technique [0002] As a soft magnetic material, magnetic nanomaterials are widely used in magnetic media storage, magnetic resonance imaging, catalysts and other fields. Iron tetroxide has attracted much attention due to its good magnetic properties and biocompatibility. Using an external magnetic field can make The particles containing nanometer ferroferric oxide achieve directional movement. At present, the preparation methods of ferric ferric oxide mainly include chemical co-precipitation method, microemulsion method, thermal decomposition method, solvothermal synthesis method and so on. Synthesis of magnetic particles by co-precipitation is the most commonly used method. Although this method is simple to operate, the obtained particles do not have good dispersibili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/08B82Y40/00C12N9/42C12N11/14C12Q1/00C12Q1/34C12Q1/54G01N27/327
CPCC01G49/08B82Y40/00C12N11/14C12N9/2445C12Y302/01021C12Q1/34C12Q1/54C12Q1/002C12Q1/006G01N27/3278C01P2004/64
Inventor 陆钢曹煦欣江瑞芬卢奎江陈俊杰
Owner JINAN UNIVERSITY
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