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Wound dressing as well as preparation method and application thereof

A wound and coating technology, applied in dressings, non-adhesive dressings, pharmaceutical formulations, etc., can solve the problems of poor antibacterial effect, easy to tear, easy to fall off, etc., achieve good antibacterial effect, accelerate wound healing, high The effect of mechanical strength

Pending Publication Date: 2021-08-24
南雄阳普医疗科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional dressings such as sterile gauze can no longer meet the needs of difficult-to-heal wounds because they cannot keep the wound moist and are easy to fall off.
However, natural biological dressings, such as autologous skin, are limited to some clinical applications due to various defects such as limited sources and inability to establish an effective blood supply in the later stage, which affects wound healing.
[0003] At present, polymer dressings are more and more applied to the field of wound healing. In the prior art, pure silk dressings have poor toughness and are easy to tear; although the pulling force of silk fibroin / polycaprolactone nanofiber membranes is much greater than that of pure silk membranes , but the antibacterial effect of silk fibroin / polycaprolactone nanofiber membrane is poor, and the hydrophilicity still cannot meet the demand, resulting in poor biocompatibility of silk fibroin / polycaprolactone nanofiber membrane and easy to fall off from the wound surface

Method used

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  • Wound dressing as well as preparation method and application thereof
  • Wound dressing as well as preparation method and application thereof
  • Wound dressing as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
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preparation example Construction

[0036] In a second aspect, the present invention provides a method for preparing a wound dressing, comprising the following steps: performing electrospinning on the silk fibroin-polycaprolactone blend solution, soaking the electrospinning product in ethanol for exchange The cross-linked product is the fiber scaffold; the fiber scaffold is alternately soaked in chitosan aqueous solution and collagen aqueous solution with opposite electrical properties to form a coating layer on the surface of the fiber to obtain a wound dressing.

[0037] As a preferred technical solution, the weight ratio of silk fibroin to polycaprolactone in the silk fibroin-polycaprolactone blend is 1:1, the solvent is hexafluoroisopropanol, and the concentration is 7wt%.

[0038] As the preference of the above-mentioned technical scheme: the coating layer is prepared by the following method: the fiber support is alternately put into a positively charged aqueous solution of chitosan and soaked in a negativel...

Embodiment 1

[0042] Embodiment 1: Preparation of silk fibroin-polycaprolactone blend

[0043] (1) Bombyx mori cocoon shells are boiled in sodium carbonate solution to remove sericin to obtain pure silk fibers;

[0044] (2) dissolving the silk fibroin fiber obtained in step (1) in a 9.3mol / L lithium bromide solution to obtain a crude silk fibroin solution;

[0045] (3) Pour the crude silk fibroin solution prepared in step (2) into a dialysis bag of 8000-14000 Da, and dialyze for 7 days at 2-8°C;

[0046] (4) Take out the crude silk fibroin solution after dialysis, centrifuge at a speed of 8000r / min, remove the impurities precipitated at the bottom of the centrifuge tube, and obtain a pure silk fibroin solution;

[0047] (5) freeze-drying the silk fibroin solution obtained in step (4) to obtain solid silk fibroin powder;

[0048] (6) Weigh 1.75g ​​silk fibroin powder and 1.75g ​​polycaprolactone, mix the two and dissolve them in 46.50g hexafluoroisopropanol (HFIP), seal and place on a magn...

Embodiment 2

[0049] Embodiment 2: preparation fiber support

[0050] (1) Take 4 injection tubes, cut off a certain length of needle, and smooth the needle tip with sandpaper, so that the remaining length of the needle is about 1.2cm;

[0051] (2) 8 mL of the silk fibroin-polycaprolactone blend solution prepared in Example 1 were extracted respectively by 4 injection tubes, and the 4 injection tubes were placed on the middle position of the propulsion pump, and the propulsion speed of the propulsion pump was set to be 1 mL / h, Start the propulsion pump until a little liquid flows out of the syringe;

[0052] (2) Spread the aluminum foil on the receiving roller and place it at a distance of 12cm from the needle tip; connect the two needles with a copper wire and then connect the high voltage positive electrode, and the roller is grounded;

[0053] (3) Turn on the roller power switch to make the roller rotate at a speed of 500r / min; start the propulsion pump, turn on the high-voltage power sw...

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Abstract

The invention discloses a wound dressing and a preparation method and application thereof. The wound dressing comprises a fiber scaffold and a coating layer wrapping the fiber surface, wherein the fiber scaffold is formed by crosslinking an electrostatic spinning product of a silk fibroin-polycaprolactone blended solution, and the fiber diameter of the fiber scaffold is 0.1-1 micron; and the coating layer is composed of chitosan layers and collagen layers which wrap alternately, and the outermost layer of the coating layer is the collagen layer. The prepared wound dressing has good mechanical strength, hydrophilicity and bacterium inhibition effect, the tensile strength of the wound dressing can reach 9.79 + / -0.65 MPa, the elongation at break can reach 31.94 + / -11.42 MPa, the water contact angle can be as low as 22.28 degrees, the wound dressing is high in porosity, good in air permeability and high in water absorption rate, and can accelerate wound healing, and a wound can be healed by 80% or above after the wound dressing is used for 7 days.

Description

technical field [0001] The invention relates to the field of wound dressings, in particular to a wound dressing and its preparation method and application. Background technique [0002] The skin is an important barrier to prevent the invasion of microorganisms and maintain the stability of the internal environment of the body. Burns (thermal, electrical or chemical burns), large skin defects caused by mechanical forces and other skin defect injuries will cause loss of skin barrier function. Use dressings to cover the wound surface, temporarily As a protective barrier on the body surface, it is an important measure for clinical treatment to promote wound healing. Traditional dressings such as sterile gauze can no longer meet the needs of difficult-to-heal wounds due to their shortcomings such as not being able to keep the wound moist and easy to fall off. However, natural biological dressings, such as autologous skin, are limited to some clinical applications due to various ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06N3/12
CPCD06N3/12D06N7/00D06N3/0011D04H1/4382D04H1/728D01F8/02D01F8/14A61L15/32A61L15/26A61L15/28A61L15/325A61L15/42A61L15/44A61L15/46D06N2209/141D06N2209/1671D06N2209/123D06N2211/18A61L2300/404A61L2300/412A61L2300/236A61L2300/252A61F13/01046A61F13/01017A61F13/01038A61F13/01042C08L89/00C08L67/04C08L5/08
Inventor 蒋朝俊陈万能何俊杰钟永清钟雄福
Owner 南雄阳普医疗科技有限公司