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Bacillus coagulans and application thereof

A technology of Bacillus coagulans and microbial agent, which is applied in the field of microorganisms, can solve the problems of not paying too much attention to Bacillus coagulans, the difference in performance types and effects of Bacillus coagulans strains, and not mentioning other properties of Bacillus coagulans, etc., to achieve inhibition Excellent effect of pathogenic bacteria growth, high survival rate, and ability to produce lactic acid

Active Publication Date: 2021-10-26
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus coagulans CCTCC NO.M 2019529 of CN110511884A has good heat resistance and storage resistance. The survival rate of Bacillus coagulans in the feed high-temperature granulation process is over 80%, and the survival rate of Bacillus coagulans is not less than 95% after one year of storage at room temperature ; This public document does not mention other properties of Bacillus coagulans, and the storage stability of this bacterium also needs to be improved
The Bacillus coagulans CGMCC No.21265 disclosed by CN112592870A has thermal stability, bacteriostasis and resistance to artificial gastrointestinal juice, has the ability to secrete protease, amylase and lactase, etc., and has the ability to degrade nitrite; Not paying much attention to other properties of Bacillus coagulans
However, the different Bacillus coagulans strains differed significantly not only in the type of properties and effects

Method used

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  • Bacillus coagulans and application thereof
  • Bacillus coagulans and application thereof
  • Bacillus coagulans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Screening of Bacillus coagulans

[0054](1) First crush the pickled cabbage, add 15mL sterile water, shake vigorously for 20min, then act in a water bath at 80°C for 20min; The formula is: yeast extract 10.0g / L; beef extract 10.0g / L; peptone 10.0g / L; sodium bicarbonate 1.5g / L; potassium dihydrogen phosphate 1.5g / L; magnesium sulfate heptahydrate 1.0g / L; sulfuric acid Manganese 0.1g / L; calcium chloride 1.0g / L; deionized water 1L), cultured at 45°C for 48h, and selected strains with large colonies and fast growth were further separated and purified by streaking.

[0055] (2) Preserve the isolated and purified bacterial strain obtained in the previous step in a glycerol tube; then take the bacterial liquid and inoculate it in the MRS medium, and cultivate it until the cell concentration is 10 8 cfu / mL, then according to the final concentration of 10 7 The added amount of cfu / mL was added to a solution with a bile salt content of 0.5%, incubated at 37°C for 24 h...

Embodiment 2

[0059] Example 2 Identification of Bacillus coagulans BC2000

[0060] Bacillus coagulans BC2000 was identified by 16SrDNA sequencing for taxonomic molecular identification, and the sequencing results were compared with Blast in NCBI. It was found that its 16SrDNA base sequence had the highest similarity with Bacillus coagulans, and it was identified as Bacillus coagulans. Bacillus coagulans BC2000 was deposited on January 13, 2021 in the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.21621, and the preservation address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing.

Embodiment 3

[0061] Example 3 Preparation of Bacillus coagulans CGMCC No.21621 bacterial agent

[0062] 1. Preparation of liquid bacterial agent

[0063] method one:

[0064] Inoculate a single colony of Bacillus coagulans CGMCC No.21621 in the MRS seed medium, culture at 37°C for 36 hours, then transfer to the fermentation medium, culture at 200r / min at 37°C for 40-56 hours, and the number of bacteria obtained is 1.0× 10 8 -5.0×10 9 CFU / mL fermentation broth.

[0065] Method Two:

[0066] (1) Activation of slant bacteria

[0067] Streak inoculation on slant medium (composition in g / L: peptone 10, beef extract 3, NaCl 5, bran 10, agar 15-20, pH 7.0-7.2), culture at 30°C for 24-48h;

[0068] (2) Shake flask and seed tank culture

[0069] Scrape and wash the mature bacteria sludge on the inclined surface with sterile water, put it into a sterile Erlenmeyer flask filled with glass beads and oscillate to disperse the bacteria sludge to obtain a uniform bacterial suspension, and heat the...

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Abstract

The invention discloses bacillus coagulans and application thereof, and belongs to the technical field of microorganisms. The strain has various excellent characteristics of high temperature resistance, cholate resistance, acid resistance, good storage stability, rich lactic acid production and enzyme production, capability of degrading nitrite, inhibiting growth of pathogenic bacteria, capability of degrading various mycotoxins and the like. The Bacillus coagulans CGMCC No.21621 is wide in application range, and can be applied to the industries of animal husbandry, aquaculture industry, food, medicine, feed and the like.

Description

technical field [0001] The invention relates to a bacillus coagulans and an application thereof, belonging to the technical field of microorganisms. Background technique [0002] Probiotics are defined by the World Health Organization as "live microorganisms that, when administered in adequate amounts, are beneficial to the health of the organism". There are countless supplemental probiotics available worldwide, but most are unstable at room temperature and lack tolerance to stomach acid and bile salts. At present, only low-temperature yogurt and a small amount of solid beverage granules are available in China for the intake of active probiotics. These products account for a very small share in the entire food industry. The reason why probiotic foods are small is not because people have not discovered the value of them, but because they are limited by the biological characteristics of traditional probiotics themselves. As a kind of bacteria, probiotics will metabolize and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20C12R1/07
CPCC12N1/20A23L5/28Y02A50/30Y02P60/87
Inventor 金龙倪伟超胡丹陈祥松吴金勇袁丽霞郑洁姚建铭
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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