Preparation and application of aerobic denitrification bacteria and its agent
A bacterial agent and oxygen removal technology, which is applied in the field of preparation and application of aerobic denitrification bacteria and its bacterial agent, can solve the problems of accumulation and limitation of wide application, and achieve high biomass, high denitrification efficiency, and bacterial agent less effect
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Embodiment 1
[0042] Acinetobacter YTLJ-N-S10 ( Acinetobacter sp. YTLJ-N-S10) isolation and identification
[0043] 1. Isolation of strains
[0044] The tail water from a seawater fish farming system in the coastal area was used as the stock solution, and was inoculated into a sterilized propagation medium at a 3% inoculum amount. After culturing at 30 °C for 3-7 days, it was diluted and cultured in LB medium, and a single colony was selected. Streak separation was carried out on the agar solid medium to obtain the purified strain YTLJ-N-S10 ( Acinetobacter sp. YTLJ-N-S10).
[0045] 2. Identification of strains
[0046] 2.1. Morphological identification
[0047] The bacterial strain isolated and purified in the above step 1 was analyzed and found to belong to Acinetobacter.
[0048] 2.2 Homology analysis of 16S rDNA sequence
[0049] The colony PCR method was used to amplify the 16S rDNA fragment of the strain obtained in step 1, and the results of amplifying the 16S rRNA gene fra...
Embodiment 2
[0053] Bacillus YTLJ-N-B38 ( Bacillus sp. YTLJ-N-B38) isolation and identification
[0054] 1. Isolation of strains
[0055] Inoculate littoral saltwater prawn feces residues into sterilized propagation medium according to 1% inoculum amount, culture at 35 °C for 5-7 days, then dilute culture in LB medium, and select single colony on agar solid medium Streak separation on the above to obtain the purified strain YTLJ-N-B38 ( Bacillus sp. YTLJ-N-B38).
[0056] 2. Identification of strains
[0057] 2.1. Morphological identification
[0058] The bacterial strain isolated and purified in the above step 1 was analyzed and compared that the bacterial strain belonged to Bacillus.
[0059] 2.2 Homology analysis of 16S rDNA sequence
[0060] The colony PCR method was used to amplify the 16S rDNA fragment of the strain obtained in step 1, and the results of amplifying the 16S rRNA gene fragment and cloning and sequencing showed that the 16S rDNA of the strain had the nucleotide...
Embodiment 3
[0064] Take 500 mL of simulated biogas slurry from livestock and poultry breeding and put it in an Erlenmeyer flask. The ammonia nitrogen concentration of the simulated biogas slurry is 200 mg / L, pH=7.06, C / N ratio is 1, and salinity is 0.2%. The pure Acinetobacter YTLJ-N-S10 bacterial liquid was added to the simulated biogas slurry at a constant temperature of 30 °C for 7 days at a constant temperature of 30 °C. The concentration of ammonia nitrogen dropped to 36 mg / L, and the removal rate reached 82%.
[0065] The pure bacterial liquid of Bacillus YTLJ-N-B38 was added to the high-concentration organic wastewater of simulated food processing according to the inoculation amount of 5%. mg / L, C / N ratio is 50. After 14 days of treatment at a constant temperature of 28°C, the concentrations of COD, ammonia nitrogen, and nitrate nitrogen decreased to 150, 4, and 0.5 mg / L, respectively, and the removal rates reached 92.5%, 90.0%, and 90.0%, respectively.
[0066] Then, the titratio...
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