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Saccharomyces cerevisiae culture rich in active probiotics, preparation method and special equipment thereof

A technology of active probiotics and cultures, applied in the field of feed, can solve the problems of low drying output, large steam consumption, large power consumption, etc., and achieve the effect of shortening the fermentation time

Pending Publication Date: 2021-12-24
陕西杨凌富仕特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Another object of the present invention is to provide a special equipment active drying tower for low-temperature drying of yeast culture, which solves the problems of low drying output and high power consumption due to structural factors in the drying of materials with high heat sensitivity. , The disadvantage of large steam consumption, through the present invention to achieve high drying output, reduce power consumption and steam consumption, adapt to large-scale production, suitable for drying materials with high heat sensitivity

Method used

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  • Saccharomyces cerevisiae culture rich in active probiotics, preparation method and special equipment thereof
  • Saccharomyces cerevisiae culture rich in active probiotics, preparation method and special equipment thereof
  • Saccharomyces cerevisiae culture rich in active probiotics, preparation method and special equipment thereof

Examples

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Embodiment 1

[0035] Example 1: Preparation of mixed bacterial liquid

[0036] The selected strains of yeast culture produced by this patent are Saccharomyces cerevisiae (strain number Saccharomyces cerevisiae CICC 1355), Candida utilis CICC 31188, Bacillus coagulans (strain number Bacillus subtilis CICC 10071), plant milk Bacillus (strain number Lactobacillus plantarum CICC 21790), the two yeast strains use PDA medium, Bacillus coagulans and Lactobacillus plantarum use LB and MRS medium respectively, and the equipment used is controllable temperature, rotation speed, pH, solution. Oxygen fermenter, four strains culture conditions are shown in Table 1. After the cultivation was completed, the viable bacterial contents of the four bacterial strains were all >10. 10 CFU / mL. Mix 1-2 parts of Saccharomyces cerevisiae, 0.8-1.5 parts of Candida utilis, 0.2-0.4 parts of Lactobacillus plantarum and 0.2-0.4 parts of Bacillus coagulans to prepare a mixed bacterial liquid.

[0037] Table 1 Four str...

Embodiment 2

[0039] Example 2: Preparation of solid state fermentation premix

[0040] The raw materials, components and functions used in this patent are as follows:

[0041] Mixed bacterial liquid of Example 1: 2.2-4.3 parts.

[0042] Main fermentation raw materials (providing slow-acting carbon sources, slow-acting nitrogen sources, phosphorus elements, trace elements, etc.): 40-60 parts of sprayed corn husks; 10-20 parts of feed-grade jujube powder; 10-20 parts of bran; 1-5 servings.

[0043] Fast-acting carbon source: 2-6 parts of glucose; 1-5 parts of flour.

[0044] Fast-acting nitrogen source: 1-3 parts of feed-grade urea.

[0045] Phosphorus source: 1-3 parts of calcium hydrogen phosphate.

[0046] Source of trace elements: 0.1-0.3 part of ferrous sulfate; 0.1-0.3 part of manganese sulfate; 0.1-0.3 part of magnesium sulfate.

[0047] Source of chloride ion: 0.5-2 parts of feed-grade sodium chloride.

[0048] Raw materials for pH adjustment: 0-3 parts of stone powder, 0-1 par...

Embodiment 3

[0050] Example 3: Preparation of yeast cultures

[0051] 1) Solid state fermentation:

[0052] The solid-state fermentation premix prepared from the above mixed bacterial liquid and fermentation raw materials is mixed with water in a ratio of 7:3, and a square fermentation pile with a length of more than 2 meters, a width of more than 2 meters, and a height of 0.5-0.8 meters is stacked on the fermentation bed.

[0053] like figure 1 As shown, the fermentation bed includes a fermentation bed area 7 and an outer wall 1 of the fermentation bed, and a temperature-adjusting water pipe 5 is laid on the lower layer; a radiant film 3 is laid under the water pipe, and a thermal insulation board 6 is laid under the radiation film 3; above the temperature-adjusting water pipe 5 A 4cm cement layer 4 is laid, floor tiles 2 are laid above the cement layer 4, and above the floor tiles 2 is the fermentation bed area 7, and the fermentation bed outer wall 1 is provided around the fermentation...

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Abstract

The invention provides a saccharomyces cerevisiae culture rich in active probiotics, a preparation method and special equipment thereof. The saccharomyces cerevisiae culture is prepared by mixed fermentation of mixed bacterial liquid and fermentation raw materials, wherein the mixed bacterial liquid comprises saccharomyces cerevisiae, candida utilis bacterial liquid, lactobacillus plantarum bacterial liquid and bacillus coagulans bacterial liquid; and the viable bacterium content of the bacterial liquid of the four strains is more than 10<10> CFU / mL. By reasonably using a carbon source, a nitrogen source, a phosphorus source and trace elements, the fermentation time is shortened to 70 h, and the content of effective components of the saccharomyces cerevisiae culture taking agricultural and by-products as main fermentation raw materials is increased. By improving a fermentation process and fermentation drying equipment, the content of active probiotics in the saccharomyces cerevisiae culture can be effectively increased, the content of active saccharomycetes (including saccharomyces cerevisiae and candida utilis) in a finished product of the saccharomyces cerevisiae culture produced by using the method and the equipment is greater than 9.0 * 10<8> / g, the content of active lactobacillus plantarum is greater than 1.0 * 10<4> CFU / g, the content of active bacillus coagulans is greater than 1.0 * 10 <8> CFU / g, and the total number of viable bacteria is greater than 1.0 * 10<9> CFU / g.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to a yeast culture containing active probiotics, a preparation method and special equipment thereof. Background technique [0002] In December 2013, the Ministry of Agriculture and Rural Affairs (formerly the Ministry of Agriculture) announced No. 2038 that new yeast-derived feed additives such as Saccharomyces cerevisiae culture (YC) and Saccharomyces cerevisiae cell wall were added to the "Catalogue of Feed Materials". Yeast culture is a product that is processed together with the substrate after fermentation by yeast on a specific medium under certain process conditions by liquid, solid or combined methods according to the theory of microbial metabolism and biological fermentation engineering technology. . As a feed additive, yeast culture has attracted widespread attention due to its advantages of no toxic side effects, no residual pollution, no drug resistance, and partial substi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K10/30A23K20/163A23K20/10A23K20/26A23K20/20F26B17/22F26B21/00F26B25/02F26B25/00
CPCA23K10/12A23K10/37A23K10/30A23K20/163A23K20/10A23K20/26A23K20/30F26B17/22F26B21/001F26B25/02F26B25/002Y02P60/87
Inventor 席联敏李延军叶丙奎王智伟
Owner 陕西杨凌富仕特生物科技有限公司
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