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Delftia sp. NLG11 for degrading tobacco nicotine and application of Delftia sp. NLG11

A technology of Delft bacteria and nicotine, which is applied in the field of agricultural microorganisms, can solve the problem of low initial concentration, achieve the effect of improving aroma quality, good application prospects, and reducing smoke concentration and energy

Active Publication Date: 2022-01-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The microbial bacteria that tolerate and degrade nicotine are mainly Arthrobacter and Pseudomonas. The initial concentration of these microbial bacteria that are currently isolated to degrade nicotine is relatively low. The degradation of
Patent CN107841474B discloses the application of Delftia lacustris ZJU-B1 strain to the prevention and treatment of rice false smut. This strain has not been used in the research of tobacco, and the strain can withstand high nicotine concentration and high degradation efficiency after screening. The new strain is a problem that needs to be solved at present, and it also provides more and more efficient choices of excellent strains for microbial degradation of nicotine

Method used

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  • Delftia sp. NLG11 for degrading tobacco nicotine and application of Delftia sp. NLG11
  • Delftia sp. NLG11 for degrading tobacco nicotine and application of Delftia sp. NLG11
  • Delftia sp. NLG11 for degrading tobacco nicotine and application of Delftia sp. NLG11

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Experimental program
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Embodiment 1

[0036] The isolation of embodiment 1 bacterial strain

[0037] 1. Preparation of selective isolation medium

[0038] LB medium: 10g peptone, 5g yeast extract, 10g NaCl, 15g agar, dissolved in 1L sterile ddHO 2 O water, adjust the pH to 7.0.

[0039] EB medium: 3g beef extract powder, 10g tryptone, 15g yeast powder, 5g sodium chloride, 10g glucose, 15g agar, dissolved in 1L sterile water, adjust pH to 7.0.

[0040] NA medium: 10g tryptone, 3g beef extract powder, 5g sodium chloride, 15g agar, dissolved in 1L sterile water, adjust the pH value to 7.0.

[0041] 2. Bioassay and sampling

[0042]The 15-day rice with uniform growth was selected and divided into two groups with three replicates in each group, with 4 to 6 rice in each replicate. The rice seedling dipping method was used for the bioassay of brown planthopper: pull out the rice by the root, rinse it with clean water, and dry it in a cool place until the surface is dry. water, wrap the rice root with moist cotton, p...

Embodiment 2

[0047] The identification of embodiment 2 bacterial strains

[0048] 1. Colony Morphological Characteristics

[0049] From the morphological identification, after being placed on the LB plate in an incubator at 37°C for 24 hours, the colonies were round, flat, yellow, opaque, smooth in surface and regular in edges. This bacterium is facultatively aerobic, with an optimum growth temperature of 30°C and low nutritional requirements, and grows well on common medium LB.

[0050] 2. Determination of physiological and biochemical characteristics

[0051] The results of the physiological and biochemical properties of the intestinal isolates are shown in Table 1: for rhamnose, mandarin, amygdalin, arabinose, β-galactosidase, arginine dihydrogenase, ornithine Decarboxylation, citric acid, glucose, mannitol, sorbitol, sucrose can be utilized. Cannot decompose H2S, urease, tryptophan deaminase, indole, 3-hydroxybutanone, gelatinase, lysine decarboxylase.

[0052] Table 1 Physiologica...

Embodiment 3

[0062] Example 3 Screening of bacterial strain NLG11 in nicotine medium

[0063] Mineral salt basal medium (MSM): 13.3g K 2 HPO 4 , 4.0g KH 2 PO 4 0.1 g(NH 4 ) 2 SO 4 , 1.0g yeast powder, 10.0mL trace elements (1.0g MgSO 4 ·7H 2 O, 0.4g MnSO 4 ·H 2 O, 0.2g CaCl 2 2H 2 O, 0.2g CuCl 2 2H 2 O, 0.02g FeSO 4 ·7H 2 O, dissolved with 0.1mol / L HCl to 100mL), added distilled water to make up to 1L.

[0064] Nicotine medium: 90% nicotine is filtered with a 0.22 μm filter membrane, and added to the sterilized inorganic salt basic medium.

[0065] The activated NLG11 strain was plated on MSM solid medium supplemented with 200mg / L nicotine, cultured upside down in a 30°C incubator for 24 hours, and the growth of colonies in the petri dish was observed. The results were as follows: image 3 . Depend on image 3 It can be seen that the NLG11 strain can grow a single colony in the MSM solid with 200 mg / L nicotine added, and the colony is transparent.

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Abstract

The invention discloses a Delftia sp. NLG11 for degrading tobacco nicotine and application of the Delftia sp. NLG11. The strain is preserved in Guangdong Microbial Culture Collection Center on June 28, 2021, and the preservation number of the strain is GDMCC NO: 61747. Researches show that the Delftia sp. NLG11 strain has good degradation capability on nicotine at different pH values and temperatures; the maximum degradation capability of the Delftia sp. NLG11 strain is 70.1%; the Delftia sp. NLG11 strain can reduce the concentration and strength of smoke, improves comfort of the smoke, and meanwhile,improves the formula applicability of tobacco leaves. The invention provides the excellent strain capable of degrading tobacco nicotine, and the strain has good application value in tobacco industry and environmental protection.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, and more specifically relates to a strain of Delftia NLG11 degrading tobacco nicotine and its application. Background technique [0002] In recent years, the appearance quality of tobacco leaves in some smoking areas in my country has approached the international level, but there is still a certain gap in terms of its internal quality. One of the more common and prominent problems is the high nicotine content. How to reduce the nicotine content in these tobacco leaves is a practical problem faced by various cigarette enterprises. [0003] Nicotine, also known as nicotine (nicotine), is a unique and most important alkaloid in various tobacco plants. It is extremely active and is the main component of tobacco alkaloids. Nicotine is one of the important factors affecting the quality of tobacco leaves. If the content of nicotine in tobacco leaves is too high, it will increase the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/01
CPCC12N1/20A24B15/20Y02A50/20
Inventor 金丰良许小霞王雪梅黄玲洪莹莹
Owner SOUTH CHINA AGRI UNIV
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