Bacteria enzyme compound feed additive as well as preparation method and application thereof

A feed additive and composite technology, applied in application, animal feed, animal feed, etc., can solve the problems of large amount of addition, weak antibacterial effect, unstable use effect, etc.

Pending Publication Date: 2022-05-10
江西好实沃生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Single live bacteria or compound live bacteria preparations have appeared on the market, and there are also compound additives made of live bacteria, enzyme preparations, antimicrobial peptides, and Chinese herbal medicines. However, these products on the market have unstable effects (weak antibacterial power and diarrhea rate High), large amount of addition, high cost of use, etc., the main reason is that the antibacterial effect of the strains used is not clear, and the number and types of live bacteria entering the animal intestines are not many (unable to withstand high-temperature, high-humidity granulation of feed and stomach acid and bile salts), bacterial enzymes and other additives are unscientific. Therefore, the coated lactic acid bacteria, bacteriostatic Bacillus subtilis and Clostridium butyricum with clear antibacterial effects are mainly used to produce a large amount of antibacterial substances through oxidative decomposition. The scientific ratio of glucose oxidase supplemented by the "decomposing enzyme preparation" and the synergistic effect of bacteria and enzymes can well solve this series of problems

Method used

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  • Bacteria enzyme compound feed additive as well as preparation method and application thereof
  • Bacteria enzyme compound feed additive as well as preparation method and application thereof
  • Bacteria enzyme compound feed additive as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation method of coated lactic acid bacteria

[0029] 1.1 The preparation steps of Enterococcus faecium HEW-A588 fermentation broth are as follows:

[0030] Take the glycerol tube of Enterococcus faecium HEW-A588 preserved in liquid nitrogen, dissolve it in a water bath at 37°C for 25 minutes, separate and purify it by streaking on the MRS plate, and culture it at 37°C for 48 hours; pick and grow on the plate in the sterile room Vigorous single colony, use an inoculation loop to inoculate on a fresh MRS slant, and incubate at 37°C for 18 hours; add 2.0mL sterilized physiological saline to the test tube in a sterile room, scrape the bacterial lawn with an inoculation loop, and make a bacterial suspension; Take 0.8mL of the bacterial suspension and inoculate it into a 1L Erlenmeyer flask filled with 300mL of shaker medium, pH 6.8, 37°C, 150r / min shaking culture for 6.5h, to obtain a seed solution, the number of viable bacteria is greater than 9.0×10...

Embodiment 2

[0042] Embodiment 2: Bacteriostasis experiment of 3 kinds of living bacteria and glucose oxidase in bacterial enzyme composite additive

[0043] 2.1 Preparation of pathogenic bacteria suspension

[0044]In the sterile room, 20 μL of pathogenic Escherichia coli, Salmonella and Staphylococcus aureus purchased from glycerol tubes were inoculated into 50 mL liquid nutrient medium (composition by mass percentage: peptone 0.3%, beef extract 1%) , sodium chloride 0.5%, the balance is water, adjust the pH value to 7.0, and sterilize at 121°C for 30min), place it in a vibrating shaker and culture it dynamically at 200r / min for 18h, and make Escherichia coli, Salmonella and golden yellow respectively Staphylococcus suspension, stored in a refrigerator at 4°C, for later use.

[0045] In the sterile room, the purchased Clostridium perfringens was first streaked from the glycerol tube to the TSC (tryptone-sulfite-cycloserine agar medium) plate with an inoculation loop, and obtained after ...

Embodiment 3

[0076] Embodiment 3: A kind of preparation method of bacterium-enzyme compound feed additive

[0077] The bacterium-enzyme compound feed additive contains the following components in weight percentage: 20% of coated lactic acid bacteria, 15% of bacteriostatic Bacillus subtilis, 5% of Clostridium butyricum, 10% of glucose oxidase, 30% of bentonite, Talc 20%.

[0078]The number of live bacteria of the above-mentioned coated lactic acid bacteria is 2.0×10 10 CFU / g, the number of live bacteria of antibacterial Bacillus subtilis is 1.0×10 11 CFU / g, the number of live bacteria of Clostridium butyricum is 1.0×10 10 CFU / g; the enzyme activity of glucose oxidase is 15000U / g.

[0079] Bacteriostatic Bacillus subtilis, Clostridium butyricum, and glucose oxidase are all commercially available products.

[0080] See Example 1 for the specific preparation method of coated lactic acid bacteria.

[0081] The preparation steps of bacterium-enzyme compound feed additive are: the bentonite ...

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Abstract

The invention provides a bacterial enzyme compound feed additive and a preparation method and application thereof, and the bacterial enzyme compound feed additive comprises the following components by weight: 3-20% of glucose oxidase, 10-20% of glucose oxidase, 10-20% of glucose oxidase, and the balance of water. 11%-25% of coated lactic acid bacteria; 15%-35% of antibacterial bacillus subtilis; 1.0%-6.0% of clostridium butyricum; 15 to 30 percent of bentonite and 10 to 20 percent of talcum powder. The dosage of the bacterium-enzyme compound feed additive is small, for example, the bacterium-enzyme compound feed additive can play a remarkable role when being added into weaned piglet compound feed by 200g/t, and the specific effects are as follows: the daily gain of weaned piglets is increased by 10%, the feed conversion rate is 0.14 lower than that of a control group, and the diarrhea rate is reduced by 1/2; if 100 g/t of the compound feed is added to 50-week-old laying hens, the laying rate can be slowly reduced, and the egg breaking rate and the death and culling rate are reduced by half.

Description

technical field [0001] The invention relates to a compound functional feed additive, in particular to a bacteria-enzyme compound feed additive which is mainly composed of various feed-use live bacteria and supplemented by decomposing enzyme preparations, as well as its preparation method and application. Background technique [0002] Since July 1, 2020, feed production enterprises have stopped producing commercial feed containing growth-promoting drug feed additives, and the animal husbandry industry has officially opened the curtain of "antibiotic-free feed". According to industry surveys and analysis in recent years, the breeding industry is facing huge challenges after the ban on antibiotics: increased complaints of diarrhea, decreased production performance (concentrated performance decreased daily weight gain, increased death rate), generally increased feed costs, and the use of antibiotics for on-site breeding increase, cost increases. And by studying the enteric path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/189A23K20/28A23K50/75A23K50/60A23K50/30
CPCA23K10/18A23K20/189A23K20/28A23K50/75A23K50/60A23K50/30
Inventor 李雪平何春兰
Owner 江西好实沃生物技术有限公司
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