Vertical nanowire substrate and preparation method and application thereof
A nanowire and substrate technology, applied in the field of mass spectrometry detection, can solve the problems of high application cost, difficulty, lack of material performance mass spectrometry imaging reproducibility verification, etc., and achieve the effect of enhancing imprinting function, high light absorption ability, and improving lifespan
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Embodiment 1
[0078] Example 1NGQD@MoS 2 / Preparation of SiNWs substrate
[0079] In this example, nitrogen-doped graphene quantum dots (NGQD) assisted exfoliation of MoS 2 , to obtain stable few-layer MoS 2 After the nanosheets, they are loaded on silicon nanowires (SiNWs) to form a composite substrate, that is, NGQD@MoS 2 / SiNWs substrate, the preparation flow chart is as follows figure 1 As shown, the specific steps are as follows:
[0080] (1) Preparation of SiNWs substrate
[0081] In this step, the silicon vertical nanowire substrate was prepared by metal-assisted chemical etching. 3 The solution was reacted and etched in a fume hood at room temperature for 10 min. Wash with water repeatedly after etching, then dip in dilute HNO 3 (HNO 3 :H 2 O=1:1) for 1 h to dissolve Ag. After the etching is completed, it is thoroughly cleaned with ultrapure water, and dried at room temperature to obtain a silicon vertical nanowire substrate.
[0082] (2) Preparation of NGQDs
[0083] 1...
Embodiment 2
[0092] Embodiment 2 Preparation of different substrates
[0093] To validate NGQD@MoS 2 / SiNWs substrates are used to improve the ability of lipid detection and molecular imaging. The inventors further prepared unmodified SiNWs substrates, including MoS without NGQD exfoliation 2 SiNWs substrate - MoS 2 / SiNWs substrate and SiNWs substrate including NGQD - NGQD / SiNWs substrate.
[0094] The method for preparing the SiNWs substrate is the same as step (1) of Example 1.
[0095] Preparation of MoS 2 The method of / SiNWs substrate is basically the same as in Example 1, the difference is that the MoS is not peeled off 2 NGQD is not added to nanosheets, as follows:
[0096] (1) Prepare SiNWs substrate, same as embodiment (1).
[0097] (2) Preparation of MoS 2 nanosheet suspension
[0098] Preparation of MoS by Liquid Phase Exfoliation 2 Nanosheet suspension, the specific method is as follows: Weigh MoS 2 The solid powder was dispersed in 50% ethanol solution to make the c...
Embodiment 3
[0103] Example 3 Blot Mass Spectrometry Imaging
[0104] Freshly frozen tissues were stored in a -80°C freezer until use. Tissue embedding was performed using 2.5% carboxymethylcellulose (CMC) solution prior to cryosectioning on a microtome cryostat. Tissue sections with a thickness of 30-100 μm were obtained using a CryoStar NX50 cryostat (Thermo Fisher Scientific) for imprinted LDI-MSI analysis.
[0105] The tissue slices were placed on the pre-cooled substrate, and heated by the back of the finger to make it melt and attach to the different substrates prepared in Example 1 and Example 2. After allowing it to interact with the substrate for 1-3 minutes, the tissue layer was washed away with ultrapure water, leaving tissue imprints.
[0106] Imaging data acquisition is carried out in the mass range of m / z 200 to 1000 using positive ion or negative ion reflectance mode, the spectrogram per pixel is 500shots cumulative spectrogram, and the lateral resolution is set to 20μm-30...
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