Serratia marcescens YP1 with strong pathogenicity and strong drug resistance to fishes and application of serratia marcescens YP1

A technology of Serratia marcescens and drug resistance, applied in the field of microorganisms, can solve the problems of economic loss of aquaculture production, the advent of an effective vaccine for Serratia marcescens, etc., to achieve effective dissemination and epidemic, prevention and control of transmission and popular effects

Pending Publication Date: 2022-05-24
中国水产科学研究院北戴河中心实验站
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above reports indicate that Serratia marcescens is highly pathogenic to humans, mammals, reptiles, amphibians, insects, freshwater fish and even plants, and it is widely distributed in marine aquaculture environments. Fish are also pathogenic and have strong drug resistance, which will not only cause economic losses to aquaculture production, but may also cause some potential public health risks to aquaculture production practitioners, which should be paid enough attention
In addition, Serratia marcescens is becoming increasingly resistant to multiple antibiotics, for which there is currently no effective vaccine available

Method used

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  • Serratia marcescens YP1 with strong pathogenicity and strong drug resistance to fishes and application of serratia marcescens YP1
  • Serratia marcescens YP1 with strong pathogenicity and strong drug resistance to fishes and application of serratia marcescens YP1
  • Serratia marcescens YP1 with strong pathogenicity and strong drug resistance to fishes and application of serratia marcescens YP1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A strain of Serratia marcescens, the Latin name is Serratia marcescens, the laboratory number is YP1, isolated from the skin ulcer lesions of the diseased flounder, preserved in the China Collection of Type Cultures (CCTCC), the preservation address is Hubei No. 299, Bayi Road, Wuchang District, Wuhan City, Province, the preservation number is CCTCC NO: M 20211340.

[0029] 1. Isolation process of strains

[0030] The strain was isolated from the tissue of the surface ulcer of the flounder (body weight: 555.27±83.32 g) suffering from ascites and ulcer disease in a farm in Leting County, Hebei Province. The specific separation process is as follows: aseptically collect the tissue from the ulcer lesions on the surface of the diseased flounder, and perform tissue homogenization. Dip the tissue homogenate with a sterile cotton swab, streak it into a solid nutrient agar medium, and after culturing at 30°C for 24 hours, pick a single colony of the dominant colonies on the me...

Embodiment 2

[0051] Pathogenicity testing

[0052] 1. Flounder infection experiment

[0053] The healthy flounder (body weight: 25.20±5.0 g) used in the artificial resensitization experiment was obtained from the Beidaihe Central Experimental Station of the Chinese Academy of Fishery Sciences, obtained from a family of artificially induced gynogenesis, with high homozygosity and genetic similarity.

[0054] 210 flounders (weight: 25.20±5.0g) were randomly selected and divided into 7 groups with 30 tails in each group. They were reared in a 300L circular glass fiber reinforced plastic water tank with a water temperature of 21±1℃ and a daily water change of 50%. Start the experiment a few days later. The preparation method of the injection bacterial solution was based on Wang Lei et al. (Wang Lei, Chen Songlin, Zhang Yingping, Fan Caixia, Liu Yang, Tian Yongsheng, Deng Han, Liu Shoutang, Sun Deqiang, Xie Mingshu, Li Wenlong. Screening and analysis of three consecutive generations of flounde...

Embodiment 3

[0072] Drug sensitivity test:

[0073] According to the experimental operation standard of the Clinical Laboratory Center (NCCL) of the Ministry of Health, the sensitivity of the strain YP1 obtained in Example 1 to drugs was detected by the K-B method of paper diffusion. 8 cfu / ml) was spread on the MHA medium, and the drug sensitive patch was pasted on the medium with sterile tweezers after 5 min. After culturing at 30°C for 18 hours, the diameter of the inhibition zone was observed and measured, and the resistance of the pathogenic strains to antibiotics was determined. The susceptibility results of strain YP1 to the 32 antibiotics tested are shown in Table 4.

[0074] Table 4 YP1 drug susceptibility test results

[0075]

[0076]

[0077] Note: S: highly sensitive; I: moderately sensitive; R: resistant.

[0078] It can be concluded from Table 4 that, compared with other fish, mammals and other animal-derived strains (Serratia marcescens), the strain YP1 is more resi...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to serratia marcescens YP1 with high pathogenicity and high drug resistance to fishes and application. The bacterial strain is separated from the focus of seawater paralichthys olivaceus as a pathogenic bacterium for the first time, and the bacterial strain has strong pathogenicity to seawater, freshwater fishes and the like and has strong drug resistance. The strain is helpful for developing more accurate immune prevention and control products (vaccines) and guiding breeding production to efficiently, comprehensively and effectively prevent and control transmission and prevalence of serratia marcescens. In addition, the strain can provide a research material for research on virulence-related genes and pathogenic mechanisms of serratia marcescens; a research material is provided for the research of the serratia marcescens drug-resistant gene; a theoretical basis is provided for the prevention and treatment research of the fish ascites disease; the method is of great significance to screening of fish disease-resistant germplasm and breeding of disease-resistant varieties, and provides a research thought for prevention and treatment of serratia marcescens infection diseases of other animals.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Serratia marcescens YP1 with strong pathogenicity and strong drug resistance to fish and application thereof. Background technique [0002] Serratia marcescens belongs to Enterobacteriaceae and Serratia Bizio, widely distributed in food, respiratory tract, urinary tract, gastrointestinal tract, etc. Harmful environmental bacteria, but in recent years, it has been reported in the literature that the infection caused by this bacteria is even more serious than that of many common pathogenic bacteria. Because of its invasiveness and resistance to many commonly used antimicrobial drugs, it has become a An important opportunistic pathogen. Serratia marcescens can cause endocarditis, meningitis, pneumonia, wound infection, eye infection, sepsis, blood transfusion and postoperative infection and urinary tract infection. Serratia marcescens has become an important pathogen of nosocomial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/43
CPCA61K39/025A61P31/04A61K2039/52A61K2039/552Y02A40/81
Inventor 赵雅贤侯吉伦王桂兴王玉芬张晓彦何忠伟刘玉峰张祎桐
Owner 中国水产科学研究院北戴河中心实验站
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