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Method for producing DHA by using schizochytrium limacinum fermentation

A technology of Schizochytrium and fermentation medium, which is applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, and can solve the problems of low biomass yield and failure to meet the needs of industrialization.

Pending Publication Date: 2022-05-24
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the above prior art, although the salt concentration in the method is low, judging from the fermentation results, the yield of biomass and DHA is very low, which is far below the requirements of industrialization

Method used

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  • Method for producing DHA by using schizochytrium limacinum fermentation
  • Method for producing DHA by using schizochytrium limacinum fermentation
  • Method for producing DHA by using schizochytrium limacinum fermentation

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Experimental program
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Embodiment 1

[0031] 1. Configure the activation medium: add 100ml of the activation medium to the 500ml conical flask. The components of the activated medium are: glycerol 40g / L, glutamic acid 30g / L, yeast extract 6g / L, dihydrogen phosphate Potassium 6g / L, magnesium sulfate 6g / L, metal ion solution 1g / L, vitamin solution 1g / L. Sterilize at 121°C for 30 min, and cool for later use.

[0032] 2. Configure the seed bottle medium: add 200ml of seed culture liquid base to the 1000ml conical flask. The components of the seed culture liquid base are: glycerol 50g / L, glutamic acid 30g / L, yeast extract 9g / L, phosphate dibasic Potassium hydrogen 2.1g / L, magnesium sulfate 5g / L, diammonium hydrogen phosphate 1g / L, metal ion liquid 1g / L, vitamin liquid 1g / L. Sterilize at 121°C for 30 min, and cool for later use.

[0033] 3. Inoculate the above-mentioned activated culture solution into fresh liquid seed medium again, with an inoculation amount of 5%, at 28° C., 150-220 rpm, and cultivate for 24 hours t...

Embodiment 2

[0040] The method provided in the embodiment of the present invention is the same as that of embodiment 1, and the difference is: 1) in the fermentation system:

[0041] raw material name Fermentation medium (g / L) glycerin 50 Glutamate 45 yeast extract 4 Potassium dihydrogen phosphate 4.2 Magnesium sulfate 10 Sodium chloride 0 calcium chloride 0 Sodium sulfate 0 sodium bicarbonate 0 Ammonium sulfate 2 Potassium chloride 0 metal ionic liquid 2 Vitamin liquid 2

[0042] 2) Step 7 in this embodiment is different from Step 7 in Embodiment 1:

[0043] 7. According to the inoculation amount of 0.2‰, insert the cultured seed bottle seed liquid into the 1700L seed tank containing the above-mentioned 1000L seed tank medium, at the cultivation temperature of 28°C, the stirring speed of 200rpm, the ventilation rate of 1vvm, and the natural cultivation of pH for 24h, to obtain Seed jar. (The see...

Embodiment 3

[0048] The method provided by the embodiment of the present invention is the same as that of embodiment 1, and the difference is: in the fermentation system:

[0049]

[0050]

[0051] Two groups of parallel experiments, shake flask fermentation: sampling and testing, the average of the two batches is that the dry weight of the bacteria is 70g / L, the total oil is 41g / L, the oil content is 58.5%, and the DHA yield is 21g / L.

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Abstract

The invention provides a method for producing DHA (docosahexaenoic acid) by using schizochytrium limacinum through fermentation. The method comprises the following steps: inoculating schizochytrium limacinum into a fermentation culture medium for fermentation; the fermentation system comprises a carbon source, a nitrogen source and inorganic salt; the inorganic salt is composed of phosphate and magnesium salt. According to the method, a specific fermentation culture medium without additional supplementation of sodium / chloride ions is used for fermentation culture, growth and metabolism of thalli are not affected, total oil is obviously increased, and meanwhile, the problem of corrosion of chloride ions to equipment is effectively reduced.

Description

Technical field [0001] The present invention relates to the field of microbial fermentation, and more specifically, to a method for producing DHA using Schizochytrium fermentation. Background technique [0002] Schizochytrium, also known as Schizochytrium, is a type of marine fungus belonging to the phylum Labyrinthulomycota, the class Myxomycetes, the order Thraustochytridae, and the family Thraustochytridaceae. It is single-celled and spherical. Docosahexaenoic acid (Trans-4,7,10,15,16,19-docosahexaenoic acid, DHA for short) is an important omega-3 series polyunsaturated fatty acid and an essential unsaturated fatty acid for the human body. It has physiological functions such as brain health care, promoting vision development, anti-inflammatory immunity, lowering blood lipids, and assisting in the treatment of cardiovascular diseases and cancer. It is difficult for the human body to synthesize DHA itself and must be ingested from outside the body. Deep-sea fish oil is th...

Claims

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Application Information

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IPC IPC(8): C12P7/6434C12N1/14C12R1/645
CPCC12P7/6427C12N1/14
Inventor 李翔宇汪志明江旭赵洒刘洋唐孝鹏陆姝欢
Owner CABIO BIOTECH WUHAN CO LTD
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