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Non-diagnostic cycle signal amplification photoelectrochemical detection method for syncytial virus RNA

A circulating signal and syncytial virus technology, applied in the fields of marine monitoring, medicine, and analytical chemistry, can solve the problems of incomplete effectiveness and achieve low detection limit, excellent detection and analysis performance, and wide detection range.

Inactive Publication Date: 2022-07-29
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far there is no widely available commercial vaccine for RSV, and ribavirin and palivizumab are the only options for treating retroviral infections, but they are not completely effective

Method used

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  • Non-diagnostic cycle signal amplification photoelectrochemical detection method for syncytial virus RNA
  • Non-diagnostic cycle signal amplification photoelectrochemical detection method for syncytial virus RNA
  • Non-diagnostic cycle signal amplification photoelectrochemical detection method for syncytial virus RNA

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0044] Example 1: Analytical Properties

[0045] Powdered tungsten diselenide (WSe 2 ) were purchased from Bailingwei Technology Co., Ltd. (Beijing, China). MAX (Ti 3 AlC 2 ) was obtained from Scientific Compass Feynman Nano. (Zhejiang, China). Chloroauric acid (HAuCl 4 •4H 2 O) Ordered from Shanghai Reagent Co., Ltd. (Shanghai, China). 6-Mercaptohexanol (MCH) was ordered from Sangon Bioengineering Shanghai Co., Ltd. (Shanghai, China). 4-Aminophenyl phosphate monosodium (4-APP) was obtained from Shanghai Huicheng Biotechnology Co., Ltd. (Shanghai, China). Streptavidin-conjugated alkaline phosphatase (SA-ALP), 4-aminophenol (4-AP), N,N-dimethylformamide (DMF), squaraine (SQA), and tris(2- Carboxyethyl)phosphine (TCEP) and all other reagents were obtained from Aladdin (Shanghai, China). By adding 0.2 moles per liter of NaH 2 PO 4 and 0.2 moles per liter NaHPO 4 Phosphate buffer solutions were prepared by mixing stock solutions containing 0.1 mol per liter of potassi...

example 2

[0049] Example 2: Repeatability, Storage Stability, Selectivity

[0050] When the syncytial virus RNA concentration was 80.0 fmol / L, the repeatability of the method was investigated, such as figure 2 A shown.

[0051] The long-term storage stability of the modified electrodes was investigated by measuring syncytial virus RNA at a concentration of 80.0 fmol / L every six days. After 30 days of storage, the modified electrodes were monitored for five consecutive cycles, and the photocurrent response showed no significant change. Therefore, the modified electrode exhibits excellent stability, such as figure 2 shown in B.

[0052] The performance of the PEC method was investigated by testing the signal response of interfering substances (influenza A virus RNA (Flu-A), adenovirus RNA (ADV), human rhinovirus RNA (RV), and human hyperpneumovirus RNA (HMPV)). Optional. When 1.0 fmol / L syncytial virus RNA was used as the detection sample, a significantly improved photocurrent resp...

example 3

[0053] Example 3: Morphological Characterization of Materials

[0054] TEM characterizes WSe 2 and morphologies of AuNPs. like image 3 shown, WSe 2 presents an irregular hexagonal shape, and after peeling, WSe 2become thinner. AuNPs have a good uniform spherical shape with a size range of 6–10 nm. Ti 3 AlC 2 Presents a blocky morphology with a smooth surface. Ti 3 AlC 2 side as image 3 C, but the layered structure is not obvious. After etching MAX with lithium fluoride and hydrochloric acid, the bulk Ti 3 AlC 2 It is transformed into a typical organ-like structure, and the Al layer is peeled off to obtain an organ-like morphology in the figure, which indicates that Ti has been successfully prepared. 3 C 2 .

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Abstract

The invention belongs to the fields of analytical chemistry, marine monitoring and medicine, and particularly relates to a non-diagnostic method for detecting syncytial virus RNA (Ribonucleic Acid) through circulating signal amplification photoelectrochemistry. A carbon paste electrode is modified by gold nanoparticles, a multi-layer titanium carbide nanosheet and a tungsten diselenide nanosheet, and a front primer is modified on the electrode. Respiratory syncytial virus nucleic acid is amplified through in-situ recombinase polymerase, and an amplification product is linked with biotin and is further combined with streptavidin alkaline phosphatase. The alkaline phosphatase catalyzes hydrolysis of the p-aminophenyl phosphate monosodium salt to generate p-aminophenol; tCEP / SQA / 4-AP is used as a redox cycle signal amplification factor, a relationship between photocurrent response and syncytial virus RNA concentration is established, and a photoinduced electrochemical detection method for measuring syncytial virus RNA is constructed. The method has the advantages of low background signal, high signal-to-noise ratio, high sensitivity and good selectivity. Results of content determination of sea water, sewage and soil samples in a coastal zone show that the method is suitable for determination of the syncytial virus in the coastal zone sample.

Description

technical field [0001] The invention belongs to the fields of analytical chemistry, marine monitoring and medicine, and in particular relates to a method for non-diagnostic purpose of amplifying photoelectrochemical detection of syncytial virus RNA. Background technique [0002] Respiratory syncytial virus, a member of the genus Pneumovirus of the Paraviridae family, is one of the most important viral pathogens causing respiratory tract infections in children and the elderly. It is an RNA virus that spreads through airborne droplets and close contact with an incubation period of three to seven days. Respiratory syncytial virus infection causes a wide range of clinical manifestations, such as: otitis media, mild upper respiratory tract infection, and potentially fatal of lower respiratory tract disease. [0003] More and more 2D materials with large surface area are used as support materials for nano-precious metals to improve the electrical conductivity, biocompatibility, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 混旭郑杰高辉玉陈丹武雨菲王如昊李小花
Owner QINGDAO UNIV OF SCI & TECH
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