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Bacillus altitudinis HMQAU20091, microbial inoculum as well as preparation method and application of bacillus altitudinis HMQAU20091 and microbial inoculum

A technology of HMQAU20091, Bacillus highlandii, applied in the directions of botanical equipment and methods, biochemical equipment and methods, and applications, to achieve the effects of high inhibitory effect, high biocontrol potential, and broad antibacterial spectrum

Active Publication Date: 2022-08-09
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, there is a lack of biocontrol agents that have higher control effects on various plant diseases such as cucumber downy mildew.

Method used

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  • Bacillus altitudinis HMQAU20091, microbial inoculum as well as preparation method and application of bacillus altitudinis HMQAU20091 and microbial inoculum
  • Bacillus altitudinis HMQAU20091, microbial inoculum as well as preparation method and application of bacillus altitudinis HMQAU20091 and microbial inoculum
  • Bacillus altitudinis HMQAU20091, microbial inoculum as well as preparation method and application of bacillus altitudinis HMQAU20091 and microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Inhibitory effect of strain HMQAU20091 on mycelial growth of different pathogenic bacteria

[0046] 1. Test plant diseases and their pathogenic bacteria:

[0047] Lily Botrytis: Botrytis cinerea HMQAU200037; Bean Botrytis: Botrytis cinerea HMQAU170042; Garlic Sclerotinia: Rhizoctonia solaniHMQAU210022; Celery Sclerotinia: Sclerotinia sclerotiorum HMQAU170216; Cabbage sclerotinia: Sclerotinia sclerotiorum HMQAU180110; Tomato gray leaf spot: Stemphylium solani HMQAU170209; Wheat scab: Fusarium graminearumHMQAU170037; Grape white rot: Coniella diplodiella HMQAU170082; Bacterial blight of blueberry: Lasiodiplodia pseudotheobromae HMQAU140073; Pythium root rot of spinach: Pythium irregulare HMQAU210091, Pythium sylvaticumHMQAU210092; Phytophthora cinnamomi HMQAU180002; Lettuce leaf spot: Ulocladium chartarum HMQAU210122, Stemphylium botryosum HMQAU210121; Spinach root rot: Fusarium oxysporum HMQAU170173, Fusarium equiseti HMQAU170169; Blueberry leaves Spot: Pesta...

Embodiment 2

[0089]Example 2 Inhibitory effect of bacterial strain HMQAU20091 fermentation filtrate on different pathogenic bacteria

[0090] 1. Test method:

[0091] The test strain is the same as in Example 1; the mycelium growth rate method (Chen Nianchun, 1991) is adopted, the sterile filtrate of the fermentation broth is mixed with the cooled PDA medium (45-50° C.) after melting (diluted 10 times), and the Plate, with PDA medium added with an equal volume of LB liquid medium as a control, inoculate the pathogenic bacteria cake with a diameter of 5 mm in the center of the plate. When the control group covered 3 / 4 of the petri dish, the colony diameter of the control group and the treatment group was measured by the cross method, and the bacteriostatic rate was calculated.

[0092] Inhibition rate (%)=(control colony radius (mm)-treated colony radius (mm)) / control colony radius (mm)×100%

[0093] 2. Inhibitory effect of strain HMQAU20091 fermentation filtrate on different pathogens: ...

Embodiment 3

[0098] Example 3 Effects of strain HMQAU20091 fermentation filtrate on the release and resting of zoospores of Cucumber Downy mildew

[0099] 1. Test method:

[0100] The effect of the fermentation filtrate of Bacillus highlandii strain HMQAU20091 on the release and resting of zoospores of Cucumber Downy mildew was determined by the concave glass slide method.

[0101] Prepare a concentration of 2 x 10 in sterilized tap water 5 sporangia suspension per mL. Seven treatments were set: strain HMQAU20091 fermentation filtrate stock solution, 5 times, 12.5 times, 25 times, and 50 times; pyraclostrobin was diluted 750 times, and sterilized tap water was used as blank control. Mix each treatment agent and the sporangia suspension in equal volumes to obtain the fermentation filtrate of strain HMQAU20091 diluted 2 times, 10 times, 25 times, 50 times, and 100 times for treatment groups, and for pyraclostrobin diluted 1500 times for treatment groups. Put 20 μL dropwise on the concave ...

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Abstract

The invention provides a bacillus altitudinis strain HMQAU20091, the strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on April 14, 2022, and the preservation registration number is CGMCC (China General Microbiological Culture Collection Center) No.24677. The bacillus altitudinis strain HMQAU20091 has the advantages that the bacillus altitudinis strain HMQAU20091 can be used for preparing the bacillus altitudinis strain HMQAU20091; the invention also provides a microbial agent prepared from the bacillus altitudinis strain HMQAU20091, and the microbial agent contains the strain HMQAU20091 and / or a metabolite of the strain HMQAU20091. The invention also provides a microbial agent prepared from the bacillus altitudinis strain HMQAU20091. The invention also provides a preparation method of the microbial agent. The invention also provides application of the microbial agent. The invention further provides a method for preventing and treating plant diseases. Indoor toxicity determination proves that the strain HMQAU20091 has good inhibitory activity on various pathogenic bacteria such as cucumber downy mildew bacteria and the like. The fermentation filtrate of the strain HMQAU20091 as a biocontrol inoculant has the advantages of short life cycle and easy artificial culture, and is a biocontrol strain with strong selectivity, no pollution and environmental friendliness, and pathogenic bacteria are not easy to generate drug resistance.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a strain of Bacillus highlandi HMQAU20091, a bacterial agent and a preparation method and application thereof. Background technique [0002] There are many stubborn old diseases and some emerging diseases in agricultural production, which seriously threaten the production of field crops, vegetables, fruit trees and flowers before, during and after production. Select important plant diseases in local production and markets such as lily gray mold, bean gray mold, garlic sclerotinia, celery sclerotinia, Chinese cabbage sclerotinia, tomato gray leaf spot, wheat scab, grape white Root rot, blueberry hairy blight, spinach Pythium root rot, blueberry phytophthora root rot, lettuce leaf spot, spinach Fusarium root rot, blueberry leaf spot, yam Fusarium root rot , soybean black spot, quinoa branch blight, carrot soft rot, garlic dry rot, kiwifruit soft rot, cucumber ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 梁晨沈莲花李守望赵洪海
Owner QINGDAO AGRI UNIV
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