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Preparation method for biologic photosensitive protein-nanometer semiconductor composite photoelectric electrode

A nano-semiconductor and bio-light technology, applied in the direction of material analysis, measuring devices, and instruments through electromagnetic means, can solve the problems of affecting the photoelectric conversion efficiency of photoelectrodes and weakening the oxidation of mediators, and achieves uncomplicated preparation methods and charge separation. Promote and promote the effect of application prospects

Inactive Publication Date: 2004-12-29
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the spontaneous electron transfer process after RC is excited and the competition reaction of reverse charge recombination that may occur at any time to return to the ground state greatly weaken the oxidation of the solution mediator by RC after photoexcitation, and then affect the entire photoelectrode. Photoelectric conversion efficiency
Although the use of nanomaterials with good energy matching can promote the effective separation of RC electron-hole pairs after photoexcitation, and reduce the negative impact of charge recombination on protein photocurrent after RC excitation to a certain extent, but this method It cannot fundamentally solve the interference of the competition reaction that occurs after the RC is stimulated to the photoelectric conversion of the entire photoelectrode

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Using spinach as a raw material, crude chlorophyll was obtained, and DEAE-Sephaerose CL-6B column chromatography was performed at 4°C. First wash with about 100ml of petroleum ether, then use petroleum ether containing 0.5% n-propanol to elute the plant pigments successively, discard the carotenoids that elute first, collect the chlorophyll a that elutes next, and put them in the N 2 After drying in medium, dissolve in 1 g / L pyridine solution and store in dark state at 4°C. Take 10ml diluted plant chlorophyll pyridine solution (0.1mmol / L), in N 2 Dry in medium, add 0.5ml glacial acetic acid. The resulting plant pheophytin in N 2 After drying in medium, dissolve in 10ml acetone and store in dark state at 4°C. A certain volume of RC solution is taken, and 10% volume of the acetone solution of the above-mentioned plant pheophytin is added, so that the molar ratio of plant pheophytin to RC is greater than 20%. After stirring, keep warm at 43.5±0.5°C for a per...

Embodiment 2

[0015] Embodiment two: adopt porphyrin n-propyl ether alcohol as replacement pigment, add the acetone solution of 10% volume replacement pigment in certain volume of RC solution, make the molar ratio of porphyrin n-propyl ether alcohol and RC greater than 25%. After stirring, keep warm at 43.5±0.2°C for a period of time, then wash off free chlorophyll with about 600ml of 0.09mol / L NaCl dilute TL buffer solution on the DEAE-cellulose chromatography column (DE-52), and use 0.15mol / L NaCl - TL buffer solution to elute the modified RC, spread the RC solution to be purified on a 10%-40% sucrose gradient, centrifuge at 4°C for 16 hours, and take the second layer of light yellow supernatant from top to bottom Liquid, that is to obtain the purified pigment replacement RC. Dilute the pigment-substituted RC with a pH 8.0 Tris-HCl buffer solution to obtain a diluted solution of mutated RC with a concentration of 2 mM, and store it in a refrigerator at 4°C. mesoporous WO 3 -TiO 2 The ...

Embodiment 3

[0016] Embodiment 3: Using porphyrin dimethyl ether diol as a replacement pigment. The preparation of the Tris-HCl buffer solution of porphyrin dimethyl ether diol replacing RC is the same as in Example 2. mesoporous WO 3 -TiO 2 The preparation of the thin film electrode is the same as that in Example 1. Pigment Replacement RC—Mesoporous WO 3 -TiO 2 The preparation of the composite photoelectrode and the measurement process of photoelectric properties are the same as in Example 2. The pigment replaces RC-mesoporous WO 3 -TiO 2 The short-circuit photocurrent of the composite photoelectrode under the above conditions is about 2.6 microamperes, which is the same as that of mesoporous WO prepared and tested under the same conditions without RC. 3 -TiO 2 3.7 times the electrode short-circuit photocurrent (0.7 microampere). The composite membrane electrode prepared above has good reproducibility. The composite membrane electrode was stored in a refrigerator at 4°C, and sho...

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PUM

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Abstract

The present invention provides one kind of effective way of constructing high performance biological light electrode. By means of modifying various mutants of extracted purple bacteria photosynthesis reaction center protein (RC) to specific nano semiconductor substrate, composite light electrode with high efficiency photoelectronic conversion function in wide wavelength range, especially in near infrared area may be obtained. On one side, these artificially modified RC has even higher photoelectronic conversion efficiency than natural RC. On the other side, adopting nano semiconductor material, especially mesoporous semiconductor material, can promote the photoelectronic conversion of RC while realizing the efficient fixation of RC. The modified and optimized RC has sensitizing effect on nano semiconductor, and this raises greatly the absorption and utilization of composite light electrode on solar energy and is favorable to developing efficient solar energy cell.

Description

technical field [0001] The invention relates to a method for preparing a modified and optimized biological photosensitive protein-nano-semiconductor composite photoelectrode in the fields of biotechnology and nanotechnology. Background technique [0002] The effective development and efficient utilization of solar energy has always been a major issue related to human survival and development, and has attracted the attention of scientific researchers from all over the world. In recent years, related nanostructures and dye-sensitized semiconductor photoelectrodes or photocells (M.Gratzel, Nature 2001, 414, 343.,) and artificially synthesized or assembled light-harvesting systems, donors, bridges, and acceptor structures Intramolecular biomimetic photosynthetic reaction center system (D. Gust, T. A. Moore, A. L. Moore, Acc. Chem. Res, 2001, 34, 40.,) has been widely reported and has become a hot spot in photoelectric conversion research. On the other hand, the design and manuf...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 孔继烈陆一东刘宝红张松徐静静
Owner FUDAN UNIV
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