Preparation method for biologic photosensitive protein-nanometer semiconductor composite photoelectric electrode

A nano-semiconductor and bio-light technology, applied in the direction of material analysis, measuring devices, and instruments through electromagnetic means, can solve the problems of affecting the photoelectric conversion efficiency of photoelectrodes and weakening the oxidation of mediators, and achieves uncomplicated preparation methods and charge separation. Promote and promote the effect of application prospects

Inactive Publication Date: 2004-12-29
FUDAN UNIV
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Problems solved by technology

However, the spontaneous electron transfer process after RC is excited and the competition reaction of reverse charge recombination that may occur at any time to return to the ground state greatly weaken the oxidation of the solution mediator by RC after photoexcitation, and then affect the entire photoelectrode. Photoelectric conversion efficiency
Although the use of nanomaterials with good energy ma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Example 1: Spinach was used as raw material to obtain crude plant chlorophyll extraction, and DEAE-Sephaerose CL-6B column chromatography was performed at 4°C. First wash with about 100ml petroleum ether, and then use petroleum ether containing 0.5% n-propanol to sequentially elute the plant pigments, discard the carotenoids that eluted first, and collect the chlorophyll a that eluted subsequently. 2 After drying in the medium, it is dissolved in 1g / L pyridine solution and stored in the dark at 4°C. Take 10ml of diluted plant chlorophyll pyridine solution (0.1mmol / L) and add it to N 2 In dry, add 0.5ml glacial acetic acid. The obtained plant pheophytin is in N 2 After drying in medium, dissolve in 10ml acetone and store in dark state at 4℃. Take a certain volume of RC solution and add 10% volume of the acetone solution of the above-mentioned plant pheophytin to make the molar ratio of plant pheophytin to RC greater than 20%. After stirring, incubate for a period of time at ...

Example Embodiment

[0015] Example 2: Using porphyrin n-propyl ether alcohol as a replacement pigment, add 10% by volume of an acetone solution that replaces the pigment into a certain volume of RC solution, so that the molar ratio of porphyrin n-propyl ether alcohol to RC is greater than 25%. After stirring, incubate for a period of time at 43.5±0.2°C, then use 0.09mol / L NaCl TL buffer dilute solution about 600ml to wash away free chlorophyll on DEAE-cellulose chromatography column (DE-52). 0.15mol / L NaCl - The modified RC was eluted with the TL buffer solution, and the RC solution to be purified was spread on a 10%-40% sucrose gradient, centrifuged at 4℃ for 16h, and the second layer of light yellow supernatant was taken from top to bottom. Liquid, that is, the purified pigment to replace RC. Dilute the above-mentioned pigment-replaced RC with a pH 8.0 Tris-HCl buffer solution to obtain a dilute solution of variant RC with a concentration of 2 mM, and store it in a refrigerator at 4°C. Mesoporous W...

Example Embodiment

[0016] Example 3: Using porphyrin dimethyl ether diol as a replacement pigment. The preparation of the Tris-HCl buffer solution of porphyrin dimethyl ether diol instead of RC was the same as in Example 2. Mesoporous WO 3 -TiO 2 The preparation of the thin film electrode is the same as in Example 1. Pigment replacement RC-mesoporous WO 3 -TiO 2 The preparation of the composite photoelectrode and the measurement process of the photoelectric properties are the same as in Example 2. This pigment replaces RC-mesoporous WO 3 -TiO 2 The short-circuit photocurrent of the composite photoelectrode under the above conditions is about 2.6 microamperes, which is a mesoporous WO without RC prepared and tested under the same conditions 3 -TiO 2 Electrode short-circuit photocurrent (0.7 microampere) is 3.7 times. The composite membrane electrode prepared above has good reproducibility. The composite membrane electrode is stored in a refrigerator at 4°C and exhibits good stability and repeatabilit...

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Abstract

The present invention provides one kind of effective way of constructing high performance biological light electrode. By means of modifying various mutants of extracted purple bacteria photosynthesis reaction center protein (RC) to specific nano semiconductor substrate, composite light electrode with high efficiency photoelectronic conversion function in wide wavelength range, especially in near infrared area may be obtained. On one side, these artificially modified RC has even higher photoelectronic conversion efficiency than natural RC. On the other side, adopting nano semiconductor material, especially mesoporous semiconductor material, can promote the photoelectronic conversion of RC while realizing the efficient fixation of RC. The modified and optimized RC has sensitizing effect on nano semiconductor, and this raises greatly the absorption and utilization of composite light electrode on solar energy and is favorable to developing efficient solar energy cell.

Description

technical field [0001] The invention relates to a method for preparing a modified and optimized biological photosensitive protein-nano-semiconductor composite photoelectrode in the fields of biotechnology and nanotechnology. Background technique [0002] The effective development and efficient utilization of solar energy has always been a major issue related to human survival and development, and has attracted the attention of scientific researchers from all over the world. In recent years, related nanostructures and dye-sensitized semiconductor photoelectrodes or photocells (M.Gratzel, Nature 2001, 414, 343.,) and artificially synthesized or assembled light-harvesting systems, donors, bridges, and acceptor structures Intramolecular biomimetic photosynthetic reaction center system (D. Gust, T. A. Moore, A. L. Moore, Acc. Chem. Res, 2001, 34, 40.,) has been widely reported and has become a hot spot in photoelectric conversion research. On the other hand, the design and manuf...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 孔继烈陆一东刘宝红张松徐静静
Owner FUDAN UNIV
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