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Genes encoding acetolactate synthase

An acetolactate synthase, gene technology, applied in genetic engineering, plant genetic improvement, lyase and other directions

Inactive Publication Date: 2005-08-17
KUMIAI CHEM IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are only a few reports on the use of resistance to PC herbicides as indicators of ALS mutant genes with specific resistance to PC herbicides.
In addition, there are only a few reports on resistance to PC herbicides and other herbicides

Method used

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  • Genes encoding acetolactate synthase
  • Genes encoding acetolactate synthase
  • Genes encoding acetolactate synthase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] [Example 1] Preparation of rice (Kinmaze) cultured cells resistant to PC herbicides

[0097] Rice husks were removed from rice seeds (variety: Kinmaze, scientific name: Oryza sativa var. Kinmaze). Seeds were soaked in 70% ethanol for 5 minutes and then in 5% antiformin for 20 minutes, followed by several washes with sterile distilled water. The seeds were then cultured statically in media containing the compositions shown in Table 3.

[0098] Inorganic salts (mixed brine for Murashige-Skoog medium)

1 pack

Thiamine Hydrochloride (0.1 g / L)

1 ml

Niacin (0.5 g / L)

1 ml

Pyridoxine hydrochloride (0.5 g / L)

1 ml

Glycine (2 g / L)

1 ml

Inositol (50 g / L)

2ml

2,4-D (200ppm)

10ml

sucrose

30 grams

Deacetylated gellan gum

3 grams

Prepare the medium to 1000 ml with distilled water and adjust the pH to 5.7

[0099] In the above medium composition, 2,4-D is a synthet...

Embodiment 2

[0107] [Example 2] The partially purified ALS protein from the resistant mutant is sensitive to herbicides

[0108] In this embodiment, the mutant ALS protein is partially purified from the resistant mutants obtained in Example 1 (except the Rb line, Sr line and Vg line, Ga line), and then the mutant ALS protein obtained is measured for herbicide activity. sensitivity. The mutant ALS protein was partially purified as follows.

[0109] First, using the composition shown in Table 3 but excluding gellan gum, 200 g or more of resistant mutants were prepared by liquid culture (without adding bispyribac-sodium). Then, use a Hiscotron in buffer solution-1 [100 mM potassium phosphate buffer (pH 7.5), which contains 20% (v / v) glycerol, 0.5 mM thiamine pyrophosphate (TPP) , 10 μM flavin adenine dinucleotide (FAD), 0.5 mM magnesium chloride and one-tenth tissue volume of polyvinylpolypyrrolidone] about 150 g of resistant mutants were homogenized. The homogenate was filtered through ny...

Embodiment 3

[0123] [Example 3] Cloning of wild-type and mutant ALS genes

[0124] In this example, the gene encoding the wild-type ALS protein (wild-type ALS gene) was cloned from the wild-type, and the gene encoding the mutant ALS protein (mutant ALS gene) was cloned from the resistant mutant.

[0125] Probes for cloning the wild-type ALS gene and the mutant ALS gene were prepared by the methods described below. Partial cDNA obtained from rice (Nippon-bare) showed high homology with maize ALS gene, and it was used as a probe in this example.

[0126] (1) Determination of the nucleotide sequence of a partial cDNA from rice (Nippon-bare) showing high homology with the corn ALS gene

[0127]As part of the rice genome project implemented by the Institute of Technology Innovation of Agriculture, Forestry and Fishery and the National Institute of Agricultural Biological Sciences, the nucleotide sequence of part of the cDNA of rice (Nippon-bare) has been determined, and the partial nucleus of ...

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Abstract

The present invention provides a gene coding for the following protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides: (a) a protein which consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8; (b) a protein which consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.

Description

field of invention [0001] The present invention relates to the gene encoding the rate-limiting enzyme-acetolactate synthase in the biosynthetic pathway of branched-chain amino acids. Background of the invention [0002] Acetolactate synthase (hereinafter referred to as "ALS") is the rate-limiting enzyme in the biosynthetic pathway of branched-chain amino acids such as leucine, valine, and isoleucine, and is known to be important for plant growth . ALS is known to exist in many species of higher plants. Furthermore, ALS has been found in various microorganisms such as yeast (Saccharomyces cerevisiae), Escherichia coli and Salmonella typhimurium. [0003] Three isozymes of ALS are known to exist in Escherichia coli and Salmonella typhimurium. Each isoenzyme is a heterooligomer consisting of a large molecular weight catalytic subunit that controls the enzyme's catalytic activity and a small molecular weight regulatory subunit that functions as a feedback inhibitor by incorpo...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N15/29C12N15/60C12N15/82
CPCC12N9/88C12N15/8274C12N15/8278C12N15/11C12N15/52
Inventor 角康一郎清水力河合清永山孝三福田笃德田中喜之
Owner KUMIAI CHEM IND CO LTD
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