The invention discloses a method for separating and extracting
cannabidiol from industrial
cannabis plants. The method comprises the following steps: leaching and concentration of the plants, extraction,
normal phase column chromatography, and reverse phase
column chromatography. The method has the following advantages of determining the polarity based on the
chemical structure characteristic of
cannabidiol, selecting suitable extraction solvents according to the principle that like dissolves like, and carrying out two times of simple
column chromatography isolation on extract which is obtained through extraction, thereby obtaining a required product. The
separation process is simple, the
cycle time is short, the number of varieties of the solvents used for formulating the polarity is small, the solvents are easy to purchase, and the solvents in the steps of extraction, column
chromatography and the like can be recycled and reused, so that the
solvent consumption is low and the cost isgreatly reduced. The
cannabidiol separated out does not need to be repurified by high-cost and high-efficiency preparative liquid
chromatography. The method has high extraction efficiency, and is convenient to implement, economical, rapid and simple in operation.