Method for continuous fermentation of glucose xylose by coupling immobilized yeast cell and pervaporation membrane

A pervaporation membrane and glucose technology, applied in the direction of supporting/immobilizing microorganisms, sterilization methods, microorganism-based methods, etc., can solve problems such as pollution, membrane separation performance degradation, etc., to reduce environmental pollution and reduce inhibition , Reduce membrane fouling and resistance

Inactive Publication Date: 2008-09-03
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Shabtai, Zhang Wei et al. (Shabtai Y et al, Biotechnology and Bioengineering, 1991, 38 (8): 869-876; Zhang Wei, etc., Membrane Science and Technology, 1997, 17 (3).) Silicone rubber / polysulfone Composite tubular membranes are used in the separation of ethanol, and there is a phenomenon that the membrane separation performance declines, and it is analyzed that this is due to membrane pollution and concentration polarization caused by dead cells; Zhong Yuehua et al. (Zhong Yuehua, Membrane Science and Technology, 2005, 25 (5).) Silicone rubber membrane is applied to continuous fermentation. Although the purpose of separating ethanol and reducing inhibition is achieved, the membrane fouling phenomenon is very serious due to the existence of free yeast cells after 80 hours

Method used

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  • Method for continuous fermentation of glucose xylose by coupling immobilized yeast cell and pervaporation membrane
  • Method for continuous fermentation of glucose xylose by coupling immobilized yeast cell and pervaporation membrane
  • Method for continuous fermentation of glucose xylose by coupling immobilized yeast cell and pervaporation membrane

Examples

Experimental program
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Effect test

Embodiment 1

[0028] In this example, calcium alginate was used to embed the cells of S. cerevisiae and Pachysomyces tannophilus.

[0029] Use two Erlenmeyer flasks to configure 200ml of cell activation medium, which contains 50g L of glucose -1 , (NH 4 ) 2 SO 4 2g·L -1 , KH 2 PO 4 2g·L -1 , yeast powder 5g·L -1 , peptone 5g·L -1 . Sterilize at 115°C for 25 minutes, cool to room temperature, and set aside. Scrape off a little of the two kinds of yeast cells stored on the slant culture medium on the aseptic operating table, put them into the respective activation medium, and activate and cultivate them on a shaking table at 30°C and 150 rpm for 48 hours.

[0030] Configuration 200ml, 60g·L -1 CaCl 2 Two bottles of solution; 400ml, 40g·L -1 A bottle of sodium alginate solution. Sterilize at 121°C for 25 minutes, cool to room temperature, and set aside. Divide the sodium alginate solution into two parts, and mix them with activated S. -1 Drop in at a rate of 60g·L -1 CaCl 2 s...

Embodiment 2

[0034] This embodiment is the proliferation and cultivation of yeast cells in a fermenter.

[0035] Cell proliferation medium contains glucose 50g L -1 , yeast extract 2g·L -1 , KH 2 PO 4 2g·L -1 , MgSO 4 0.2g·L -1 , CaCl 2 0.2g·L -1 , (NH 4 ) 2 SO 4 2g·L -1 ; Fermentation medium contains glucose 50g L -1 , xylose 25g·L -1 , yeast extract 2g·L -1 , KH 2 PO 4 2g·L -1 , MgSO 4 0.2g·L -1 , CaCl 2 0.2g·L -1 , (NH 4 ) 2 SO 4 2g·L -1 . Sterilize at 115°C for 25 minutes, cool to room temperature, and set aside.

[0036] Clean the fermentation tank, buffer tank, silicone tube, iron tube, rubber plug, etc. with detergent and soft brush, rinse with ultrapure water three times and wrap it in kraft paper, sterilize at 121°C for 25 minutes, and dry; fix the two fermentation tanks On the experimental rack, put the rubber beads embedded with yeast cells into two fermenters respectively, so that the total volume of the beads in each tank accounts for 70-80% of the v...

Embodiment 3

[0040] This embodiment is coupled with a pervaporation membrane to continuously ferment the glucose-xylose mixture and separate ethanol.

[0041] Configure fermentation medium, including glucose 50g·L -1 , xylose 25g·L -1 , yeast extract 2g·L -1 , KH 2 PO 4 2g·L -1 , MgSO 4 0.2g·L -1 , CaCl 2 0.2g·L -1 , (NH 4 ) 2 SO 4 2g·L -1 . Sterilize at 115°C for 25 minutes, cool to room temperature, and set aside.

[0042] The proliferation medium is replaced by the cell fermentation medium, and the pervaporation membrane module is respectively connected between the two serial fermenters and between the last fermentor and the recovery tank, and the cycle fermentation is carried out at 35°C. After 32 hours of fermentation, the whole device enters a steady state, and the vacuum pump is started for pervaporation to separate ethanol, and the separated ethanol vapor is condensed and collected in a product tank with a cold trap.

[0043] Experimental results: After 370 hours of ...

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Abstract

A method to continuously fermenting glucose and xylose by coupling immobilized yeast cell and pervaporation membrane is disclosed, which pertains to the field of fermentation engineering of microbial, especially relates to a ethanol production method by continuously fermenting glucose and xylose. The invention is characterized by inoculating saccharomyces cerevisiae and pachysolen tannophilus into two fermentors respectively after immobilizing the same by calcium alginate gel bead, and continuously fermenting mixed liquid of glucose and xylose by coupling immobilized yeast cell and pervaporation membrane. The effect and advantage of the invention is that by immobilizing yeasts with calcium alginate gel bead, not only cell concentration is increased, but also pollution and resistance of pervaporation membrane caused by adhesion of cells is reduce; inhibition of produced ethylene to yeast cell is reduced by pervaporation; glucose and xylose can be simultaneously fermented; materials are drawed from plant straw, not from grain. Resource is saved and environmental protection caused by burning plant straw is reduced.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, in particular to a method for coupling continuous fermentation of xylose and glucose with calcium alginate beads immobilized yeast cells and pervaporation membranes. Background technique [0002] Since the 20th century, oil as one of the main energy sources has made great contributions to the development of mankind, but oil resources are already facing depletion, and alcohol has become the most potential substitute (Su T M et al, AIChE Symposium Series, 1978, 74 (181): 75-78; Wyman C E et al, Proceedings of the Intersociety Energy Conversion Engineering Conferenc, 1994, 3: 1090-1095.), thus utilizing the research of plant fiber fermentation to produce alcohol has very important significance. Plant fiber raw materials include cellulose, hemicellulose and lignin, and its hydrolyzate is mainly a mixture of various monosaccharides and oligosaccharides such as glucose, xylose, and arabinose. It i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12N1/16C12N11/04C12M1/12C12R1/865C12R1/85
CPCY02E50/10
Inventor 刘天庆隋东宇李香琴孙相彧姜秀美
Owner DALIAN UNIV OF TECH
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