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Magnetic tumour target polymer nano vesicle and preparation method thereof

A nanovesicle and tumor targeting technology, which is applied in the fields of polymer chemistry and biomedical engineering, can solve the problems of undeveloped research on strong magnetic targeting vesicles, loss of curative effect, toxic and side effects, etc., and improve the characteristics of magnetic resonance imaging , reduce the dosage and side effects, prolong the effect of blood circulation time

Inactive Publication Date: 2009-03-25
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Anticancer drug chemotherapy is the most important means of cancer treatment except surgery. However, there are insurmountable shortcomings in tumor chemotherapy by direct drug application: the half-life of the internal circulation is too short; it has strong toxic side effects on healthy tissues and cells of the body ; It is easy to produce immune rejection in the body and reduce or even lose the efficacy of the drug
However, so far, studies on the targeting of vesicles by strong magnetic fields have not been carried out.

Method used

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  • Magnetic tumour target polymer nano vesicle and preparation method thereof
  • Magnetic tumour target polymer nano vesicle and preparation method thereof
  • Magnetic tumour target polymer nano vesicle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of folic acid-modified polymer nanovesicle carrier material folate-PEG-PDLLA

[0038] 1.1 Preparation of allyl-terminated PEG homopolymers:

[0039] The polymer is obtained by the continuous phase anionic ring-opening polymerization of ethylene oxide using potassium alkoxide as an initiator. First, 4 ml of a tetrahydrofuran solution of potassium naphthyl and 1.5 to 2.5 ml of allyl alcohol are uniformly mixed, and then stirred in a dry reaction flask for 15 minutes. Then, 20 ml of anhydrous tetrahydrofuran and 18-crown-6 in tetrahydrofuran (containing 1.5 g of 18-crown-6 and 5 ml of anhydrous tetrahydrofuran) were added under argon protection, and the mixture was cooled in an ice-salt bath after stirring for 15 minutes. , and slowly introduced a certain amount of dry ethylene oxide, and maintained the low temperature for 24 hours to make the polymerization reaction continue. Finally, it was left at room temperature for at least 3 days to facilitate complete...

Embodiment 2

[0047] Preparation of magnetic tumor-targeting polymer nano-drug-loaded vesicles

[0048] 2.1 Preparation of superparamagnetic ferric oxide SPIO nanoparticles

[0049] First, 0.7g of iron acetylacetonate, 2.9g of 1,2-hexadecanediol, 2ml of oleic acid, 2ml of oleylamine and 20ml of dibenzyl ether were mixed uniformly under nitrogen protection, heated at 200°C for two hours, and then heated to Reflux at 300°C for one hour. After the obtained black product was cooled to room temperature, reprecipitated in ethanol, centrifuged to remove excess solvent, dissolved in n-hexane and sealed for storage to obtain a hydrophobic SPIO sample solution.

[0050] The n-hexane solution of the hydrophobic SPIO prepared above was mixed with three to five times the dichloromethane solution of tetramethylamine 11-aminodecanoic acid and shaken at room temperature for 24 hours, then separated from the solution with a magnet The precipitate was washed twice with dichloromethane and separated with a ...

Embodiment 3

[0057]Testing of basic properties of magnetic tumor-targeting polymer nano-drug-loaded vesicles

[0058] 3.1 Measurement of size and morphology of magnetic tumor-targeting polymer nano-drug-loaded vesicles

[0059] The particle size of the obtained vesicles was measured by a dynamic light scattering system, and its morphology was observed and determined by transmission electron microscopy and scanning electron microscopy. The test results are shown in Figures 3 to 12 . Image 6 , Figure 7 and Figure 8 are the dynamic light scattering histograms of the corresponding vesicles, in which, it can be clearly seen from the transmission electron microscope pictures that the amphiphilic polymers self-assemble into "hollow spheres" with a central membrane wall in an aqueous solution. The wall is about 20 nm, and the hydrophobic and hydrophilic SPIOs are wrapped in the outer membrane and inner cavity of the vesicles under the interaction of hydrophilic and hydrophobic, respectively...

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Abstract

The invention discloses a magnetic tumor targeting polymer nano-capsule which contains the following ingredients by weight portion: 5-20 portions of amphiphilic copolymer of folic acid terminated polyethylene glycol and polylactic acid, one portion of paramagnetic nano-particles, a portion of hydrophilic anticancer medicine. The invention also discloses the preparation method of the nano-capsule; the preparation method adopts amphiphilic copolymer of folic acid terminated polyethylene glycol and polylactic acid as the raw material; ferroferric oxide nano-particles and hydrophilic anticancer medicine are encapsulated to obtain the nano-capsule through the ultrasonic emulsifying method. The nano-capsule can further improve the release efficiency and local concentration of the medicine, reduce the medicine dosage, the toxic and side effects. The magnetic tumor targeting polymer nano-capsule has excellent magnetic resonance imaging characteristic and has great research values and application prospects in the treatment and early diagnosis fields of cancer.

Description

technical field [0001] The invention relates to a novel drug carrier-magnetic tumor targeting polymer nanovesicle and a preparation method thereof, belonging to the fields of polymer chemistry and biomedical engineering. Background technique [0002] Cancer is one of the most important diseases that endanger human health. In China, the incidence of 59 types of cancer increased by about 45% in the 20 years from 1981 to 2000, and the incidence rate reached 245 per 100,000 people. The first cause of death for urban and rural residents. The development of more effective cancer treatment methods is of great significance to promoting the health of Chinese people. [0003] Chemotherapy with anticancer drugs is the most important cancer treatment method other than surgery. However, direct drug administration has insurmountable shortcomings in tumor chemotherapy: the half-life of circulation in the body is too short; it has strong toxic and side effects on healthy tissues and cells ...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K9/50A61K47/34A61K47/02A61P35/00
Inventor 帅心涛李皓杨小强
Owner SUN YAT SEN UNIV
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