Magnetic tumour target polymer nano vesicle and preparation method thereof

A technology of tumor targeting and nanovesicles, which is applied in the fields of polymer chemistry and biomedical engineering, can solve the problems of undeveloped research on strong magnetic targeting vesicles, loss of curative effect, toxic and side effects, etc., and improve the characteristics of magnetic resonance imaging , reduce the dosage and side effects, prolong the effect of blood circulation time

Inactive Publication Date: 2011-01-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Anticancer drug chemotherapy is the most important means of cancer treatment except surgery. However, there are insurmountable shortcomings in tumor chemotherapy by direct drug application: the half-life of the internal circulation is too short; it has strong toxic side effects on healthy tissues and cells of the body ; It is easy to produce immune rejection in the body and reduce or even lose the efficacy of the drug
However, so far, studies on the targeting of vesicles by strong magnetic fields have not been carried out.

Method used

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  • Magnetic tumour target polymer nano vesicle and preparation method thereof
  • Magnetic tumour target polymer nano vesicle and preparation method thereof
  • Magnetic tumour target polymer nano vesicle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of Folate-PEG-PDLLA as Carrier Material of Folate-PEG-PDLLA Modified Polymer Nanovesicles

[0038] 1.1 Preparation of allyl-terminated PEG homopolymer:

[0039] The polymer is obtained through the continuous phase anionic ring-opening polymerization of ethylene oxide with potassium alcoholate as an initiator. First, mix 4ml of tetrahydrofuran solution of potassium naphthyl with 1.5-2.5ml of allyl alcohol and stir in a dry reaction bottle for 15 minutes. Then add 20ml of anhydrous tetrahydrofuran and 18-crown ether-6 tetrahydrofuran solution (containing 1.5g 18-crown ether-6 and 5ml anhydrous tetrahydrofuran) under the protection of argon, and after stirring for 15 minutes, place the mixture in an ice-salt bath for cooling , and slowly feed a certain amount of dry ethylene oxide, and maintain the low temperature for 24 hours so that the polymerization reaction can continue. Finally, place it at room temperature for at least 3 days to facilitate the complete...

Embodiment 2

[0047] Preparation of Magnetic Tumor Targeting Polymer Nano-Drug-loaded Vesicles

[0048] 2.1 Preparation of superparamagnetic Fe3O4 SPIO nanoparticles

[0049] First, mix 0.7g of iron acetylacetonate, 2.9g of 1,2-hexadecanediol, 2ml of oleic acid, 2ml of oleylamine and 20ml of dibenzyl ether under nitrogen protection, heat at 200°C for two hours, and then heat up to 300°C °C for one hour. After the obtained black product was cooled to room temperature, it was reprecipitated in ethanol, centrifuged to remove excess solvent, dissolved in n-hexane and sealed for storage to obtain a hydrophobic SPIO sample solution.

[0050] The n-hexane solution of hydrophobic SPIO prepared above was mixed with three to five times the solution of tetramethylamine 11-aminodecanoic acid in dichloromethane and shaken at room temperature for 24 hours, then separated from the solution with a magnet The precipitate was washed twice with dichloromethane and separated with a magnet. Finally, hydrophi...

Embodiment 3

[0057] Testing of the Basic Properties of Magnetic Tumor Targeting Polymer Nano-Drug-loaded Vesicles

[0058] 3.1 Measurement of the size and shape of magnetic tumor-targeting polymer nano-loaded vesicles

[0059] The particle size of the obtained vesicles is measured by a dynamic light scattering system, and its shape is determined by observing and determining the transmission electron microscope and scanning electron microscope. The test results are shown in Figures 3 to 12 . Figure 6 , Figure 7 and Figure 8 are the dynamic light scattering histograms of the corresponding vesicles, in which, it can be clearly seen from the transmission electron microscope pictures that the amphiphilic polymer self-assembles into a "hollow sphere" with a central membrane wall in aqueous solution, and the membrane of the vesicle The wall is about 20nm. Under the interaction of hydrophilic and hydrophobic, the hydrophobic and hydrophilic SPIO are respectively wrapped in the outer membran...

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Abstract

The invention discloses a magnetic tumor targeting polymer nano-capsule which contains the following ingredients by weight portion: 5-20 portions of amphiphilic copolymer of folic acid terminated polyethylene glycol and polylactic acid, one portion of paramagnetic nano-particles, a portion of hydrophilic anticancer medicine. The invention also discloses the preparation method of the nano-capsule;the preparation method adopts amphiphilic copolymer of folic acid terminated polyethylene glycol and polylactic acid as the raw material; ferroferric oxide nano-particles and hydrophilic anticancer medicine are encapsulated to obtain the nano-capsule through the ultrasonic emulsifying method. The nano-capsule can further improve the release efficiency and local concentration of the medicine, reduce the medicine dosage, the toxic and side effects. The magnetic tumor targeting polymer nano-capsule has excellent magnetic resonance imaging characteristic and has great research values and application prospects in the treatment and early diagnosis fields of cancer.

Description

technical field [0001] The invention relates to a novel drug carrier-magnetic tumor-targeting polymer nanovesicle and a preparation method thereof, belonging to the fields of polymer chemistry and biomedical engineering. Background technique [0002] Cancer is one of the most important diseases that endanger human health. In China, the incidence rate of 59 types of cancer increased by about 45% in the 20 years from 1981 to 2000, and the incidence rate reached 245 people per 100,000 people. In 2006, cancer has become my country's The number one cause of death among urban and rural residents. The development of more effective cancer treatment methods is of great significance to the promotion of Chinese people's health. [0003] Anticancer drug chemotherapy is the most important means of cancer treatment except surgery. However, there are insurmountable shortcomings in tumor chemotherapy by direct drug application: the half-life of the internal circulation is too short; it has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61P35/00A61K9/00A61K47/02A61K47/34
Inventor 帅心涛李皓杨小强
Owner SUN YAT SEN UNIV
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