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Method for preparing bionic extracellular matrix silk fibroin/chitosan composite nanometer fibre

A technology of composite nanofibers and silk fibroin, which is applied in the direction of spinning solution preparation, cellulose/protein conjugated artificial filament, fiber treatment, etc., can solve the problem of silk fibroin nanofibers being brittle, poor in flexibility, and unable to realize functions Biomimetic, reducing practical value and other issues, to achieve the effect of excellent mechanical properties and degradation behavior, simple and easy preparation method, and improved flexibility

Inactive Publication Date: 2009-06-03
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Park et al[1] prepared silk fibroin nanofiber membranes by electrospinning with formic acid as a solvent, which can promote the adhesion, proliferation and differentiation of mouse preosteoblasts (MC3T3-E1), induce the production of osteocalcin, and promote bone tissue repair and regeneration, Chinese patent CN1844509A discloses "a preparation method of silk fibroin porous material", which uses water as a solvent to electrospin nanofibers with good biocompatibility and high porosity, but a single silk fibroin nanofiber Brittle, poor flexibility, reducing its practical value, and as a tissue engineering scaffold material, the best design should be biomimetic extracellular matrix in terms of structure and composition
The natural extracellular matrix is ​​composed of proteins and polysaccharides with a nanofiber network structure [2]. Adding a single natural component cannot completely simulate the composition of the extracellular matrix, so it cannot achieve functional bionics

Method used

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  • Method for preparing bionic extracellular matrix silk fibroin/chitosan composite nanometer fibre
  • Method for preparing bionic extracellular matrix silk fibroin/chitosan composite nanometer fibre

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] First add 100 grams of cocoons to 1 liter of 0.5% Na 2 CO 3 Boiling in an aqueous solution for 30 minutes, repeating the treatment three times, fully washing with distilled water, and drying in a drying oven at 45° C. to obtain degummed silk fibers. with CaCl 2 :C 2 h 5 OH:H 2 A ternary solvent was prepared with a molar ratio of O=1:2:8, and the silk fiber was hydrolyzed in a water bath at 70°C for 1 hour at a constant temperature at a bath ratio of 1:10 to obtain a completely dissolved brown-yellow silk fiber hydrolysis solution. Put the hydrolyzed solution into a dialysis bag, dialyze with distilled water for 72 hours, put the dialyzed silk fiber hydrolyzed solution into -80°C for 12 hours, and then freeze-dry it at -58°C until dry to obtain a white, odorless, loose porous solid silk fibroin.

Embodiment 2

[0028] Dissolve 1.0 gram of silk fibroin in 10 milliliters of hexafluoroisopropanol, stir until fully dissolved to obtain a silk fibroin solution with a concentration of 10% (g / ml); 0.6 gram of chitosan is dissolved in 10 milliliters of In the hexafluoroisopropanol and the trifluoroacetic acid mixed solvent that ratio 90:10 mixes, stir until dissolving completely, obtain the chitosan solution that concentration is 6% (gram / milliliter); Two kinds of solutions are with volume ratio 12:5, The mass ratio of solute is mixed at 4:1, and the mixed solution is subjected to electrospinning, spinning conditions: voltage, 20 kV; electric field distance, 130 mm; spinning rate, 80 ml / hour, to obtain an average fiber diameter of 305 nanometers Composite nanofiber membrane.

Embodiment 3

[0030] Take by weighing 1.0 grams of silk fibroin prepared in Example 1, dissolve in 10 milliliters of hexafluoroisopropanol, stir until completely dissolved, and obtain a silk fibroin solution with a concentration of 10% (g / ml); Chitosan was dissolved in 10 milliliters of hexafluoroisopropanol and trifluoroacetic acid mixed solvent with a volume ratio of 90:10, stirred until fully dissolved to obtain a chitosan solution with a concentration of 6% (g / ml); the two The two solutions were mixed at a volume ratio of 3:5 and a solute mass ratio of 1:1, and the mixed solution was subjected to electrospinning, spinning conditions: voltage, 20 kV; electric field distance, 130 mm; spinning rate, 80 ml / hour , to obtain a composite nanofibrous membrane with an average fiber diameter of 202 nm.

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Abstract

The invention relates to a method for preparing a bionic extracellular matrix silk fibroin / chitosan composite nanometer fibre. The method comprises the steps as follows: (1) cocoons without pupas are cooked in Na2CO3 solution for three times, are cleaned and dried at the temperature of 45 DEG C and are dissolved in ternary solvent to be hydrolyzed, dialyzed and freeze-dried to obtain loose porous solid silk fibroin; (2) the silk fibroin is dissolved in hexafluoroisopropanol to obtain silk fibroin solution; (3) chitosan is dissolved in mixed solvent of HFIP and TFA to obtain chitosan solution; (4) the silk fibroin solution and the chitosan solution are mixed and stirred uniformly to obtain blended electrostatic spinning solution; and (5) the mixed solution is carried out electrostatic spinning to obtain silk fibroin / chitosan composite nanometer fibre. The silk fibroin / chitosan composite nanometer fibre has good mechanical property and degradation behavior and provides best bionic physiological environment for cell growth and tissue regeneration, and the preparation method is simple and easy for implementing and is easy for realizing industrialized production.

Description

technical field [0001] The invention belongs to the field of preparation of composite nanofibers, in particular to a method for preparing bionic extracellular matrix silk fibroin / chitosan composite nanofibers. Background technique [0002] The development of tissue engineering and regenerative medicine provides an effective treatment for rebuilding or repairing human diseased tissues and organs. Degradable scaffolds provide the necessary space and support for cell growth and tissue regeneration, and provide effective channels for signal transduction, which is one of the key factors that determine the success of tissue engineering. [0003] Silk fibroin is degummed from silkworms. It is a natural protein with excellent properties such as good biocompatibility, biodegradability, good air permeability, moisture permeability, and non-immunogenicity. It is widely used in the repair and regeneration of skin, bone, cartilage, tendon, nerve conduit, blood vessel and other tissues. ...

Claims

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Application Information

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IPC IPC(8): D01F8/02D01F8/18D01D1/02D01D5/00A61L27/26A61L27/22A61L27/20
Inventor 莫秀梅张葵花王红声
Owner DONGHUA UNIV
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