Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bio-diatomite composite flocculant

A composite flocculant and biological flocculant technology, applied in the field of water treatment, can solve the problems of low treatment efficiency and secondary pollution of flocculants, achieve high treatment efficiency, reduce dosage, and improve other indicators

Inactive Publication Date: 2011-05-11
NANJING AGRICULTURAL UNIVERSITY +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a high-efficiency and environment-friendly bio-diatomite compound for the low treatment efficiency of the existing sewage treatment process, the effluent water quality needs to be improved after advanced treatment, and the addition of flocculant is easy to cause secondary pollution. flocculant

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Screening of flocculation-producing microbial strains

[0039] Take 10g of the activated sludge sample from the aerobic pool of Nanjing Suojin Village Sewage Treatment Plant, and put it in the basic salt medium (1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 , 1.0 g NH 4 NO 3 , 0.2g MgSO 4 , 1.0g NaCl / L water, pH7.0) at 30°C for 24h, filter the culture solution with sterilized filter paper, rinse the filter paper with sterile water, wash off the flocs on the filter paper, add to sterilized basal salt medium In this method, the samples were enriched and cultured for 4 times, and then the pure bacteria were isolated. The enrichment liquid was coated on Luria-Bertani (LB) medium, cultured at 30°C for 48 hours, and a single colony with a smooth surface and stickiness was selected to measure its flocculation activity. Three strains of flocculant-producing strains Providencia sp.12A (CGMCC No.3515), Stenotrophomonas sp.46C (CGMCC No.3514) and Myroides sp.122B (CGMCC No.3516...

Embodiment 2

[0041] Fermentation of flocculation-producing microorganisms: Under sterile conditions, three strains (CGMCC No.3515, CGMCC No.3514 and CGMCC No.3516) were activated on a slant, respectively, and then inoculated into LB medium shake flasks. 200r / min, culture time 50h to mid-logarithmic growth, inoculate 5% inoculum in first-level seed tanks, and scale-up culture in second-level seed tanks step by step, and the number of bacteria obtained reaches OD value of 1.0-1.2×10 9 / ml of fermentation liquid. The fermentation medium is glucose 1%, ammonium sulfate 0.15%, dipotassium hydrogen phosphate 0.05%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.002%, NaCl 0.02%, calcium chloride 0.01%, the rest is tap water, pH7.0, Fermentation at 28~30℃, tank pressure 0.5kg / cm 2 , the ventilation rate is measured by the volume of fermentation broth in m 3 / air volume m 3 ·The min meter is 1:0.8, the stirring speed is 150r / min, the pH value is about 6.5, and the aerobic fermentatio...

Embodiment 3

[0043] Centrifuge 30min at 5000rpm to the fermented liquid of stable growth phase after respectively cultivating 2d in embodiment 2, remove thallus (precipitation), concentrate (conventional ultrafiltration, decompression) supernatant, add in the supernatant and be equivalent to supernatant 0.1% CaCl by weight 2 and 2.5 times the volume of supernatant liquid ethanol, let stand at room temperature for 40 minutes, then centrifuge at 12000g for 15 minutes with a centrifuge, discard the supernatant, and obtain precipitate 1 for subsequent use; dissolve precipitate 1 with 3.0%, pH5 sodium chloride solution (sodium chloride solution just can dissolve precipitate 1 and get final product), absorb with 12% hexadecyltrimethylammonium bromide solution of 1 times sodium chloride solution volume, then obtain precipitate 2 for subsequent use through centrifugation; Dissolve the precipitate 2 with 10% sodium chloride and stir for 12 hours, add absolute ethanol 3 times the volume of the NaCl ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
specific surface areaaaaaaaaaaa
densityaaaaaaaaaa
particle size (mesh)aaaaaaaaaa
Login to View More

Abstract

The invention belongs to the water treatment field and discloses a bio-diatomite composite flocculant. The flocculant mainly contains refined diatomite and bioflocculant, wherein the weight of the bioflocculant is 10%-15% of that of refined diatomite; and the bioflocculant is an extracellular polymer prepared by selecting at least one of microbial strains with the preservation number CGMCC No. 3514, CGMCC No. 3515 and CGMCC No. 3516, fermenting, extracting, separating and purifying. The bio-diatomite composite flocculant is characterized by efficient flocculation, adsorption and filtration and environmental friend; and the flocculant can be directly added in the biochemical system of the existing sewage treatment system, thus industrial and engineering applications are easy to realize and the upgrading of the existing sewage treatment plants is also easy to realize.

Description

technical field [0001] The invention belongs to the field of water treatment, and relates to a biological-diatomite composite flocculant, in particular to a biological-diatomite composite flocculant which combines the biological flocculant and diatomite for advanced sewage treatment. Background technique [0002] Flocculation is an important process in the safe purification of water supply and wastewater treatment, and the flocculant directly affects the flocculation effect. Most of the inorganic flocculants currently on the market, such as aluminum and iron series, are toxic and affect ecological safety; organic polymer flocculants such as polyacrylamide have neurotoxicity, teratogenic, carcinogenic, and mutagenic effects, making traditional flocculants Applications are very limited. [0003] Microbial flocculants have the advantages of non-toxic, safe, biodegradable, and no secondary pollution. They have great application potential in the fields of water purification and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C02F1/52C02F1/56C02F1/28
Inventor 李顺鹏蔺嘉梅吴效祥蒋建东邹敏李荣顾文柳王融戴昕郭燕
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com