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Method for in-situ enzymolysis and recovery of cellulose system

A technology of in-situ enzymatic hydrolysis and recovery method, which is applied in chemical instruments and methods, the preparation of compounds of Group 5/15 elements of the periodic table, and the preparation of sugar derivatives, which can solve the problems of high viscosity, unobserved, and unfavorable product separation. , to achieve the effect of high recycling rate, obvious advancement and reducing consumption

Active Publication Date: 2013-01-23
青岛生物能源与过程研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large viscosity affects the mass transfer and heat transfer effects of ionic liquids, which is not conducive to the separation of products
Third, when using the "one pot" method, the recovery of ionic liquids and monosaccharides is very complicated due to the high viscosity of the system
In 2003, Rogers et al. proposed that Aqueous biphasic systems (ABS, Aqueous biphasicsystems) may be used to concentrate or reclaim ionic liquids from aqueous solutions of hydrophilic ionic liquids. However, so far, there has been no use of ABS systems to reclaim ionic liquids. However, for the more complicated problem that the system contains both polar monosaccharides and hydrophilic ionic liquids, the research on using ABS system to recover ionic liquids and separate monosaccharides has also not been reported.
In addition, in 2006, Japanese scholar Yukinobu Fukaya et al. reported that alumina columns can be used to purify [Amim][Cl] plasma ionic liquids, but for ionic liquids containing highly polar and hydrophilic ionic liquids and more polar single The complex system of sugar, and there is no report on the adsorption and separation of ionic liquids and sugars using alumina columns.

Method used

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  • Method for in-situ enzymolysis and recovery of cellulose system
  • Method for in-situ enzymolysis and recovery of cellulose system
  • Method for in-situ enzymolysis and recovery of cellulose system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Cellulose in the ionic liquid [Mmim][Me 2 PO 4 ], the dissolution temperature is 60°C, then add acetate buffer (pH=4.8), so that the volume ratio of the ionic liquid to the system is 20%, add 1.5 mg / mL cellulase, and enzymatically hydrolyze at 60°C for 24 hours , using the HPLC method to analyze the content of reducing sugar wherein, the conversion rate of cellulose into reducing sugar is 75.6%.

Embodiment 2

[0026] Cellulose in the ionic liquid [Mmim][Me 2 PO 4 ], the dissolution temperature is 60°C, then add acetic acid buffer (pH=4.8), so that the volume ratio of the ionic liquid to the system is 20%, and the organic solvent DMSO mass ratio is added to be 1%, and 1.5 mg of cellulase is added / mL, enzymatically hydrolyzed at 60°C for 24 hours, and analyzed the content of reducing sugar therein by HPLC method, and the conversion rate of cellulose into reducing sugar was 82.2%.

Embodiment 3

[0028] Concentrate the ionic liquid / solvent system after in situ enzymatic hydrolysis, which contains ionic liquid [Mmim][Me 2 PO 4 ] and a monosaccharide with a mass ratio of 12.5%, use neutral alumina (200-300 mesh) to pack a chromatographic column, the column height-to-diameter ratio is 15, first elute with methanol, and the elution flow rate is 1.0mL / min, use The ultraviolet spectrophotometer detects whether the elution component contains ionic liquid at a wavelength of 220nm, and analyzes the glucose in the eluent by the DNS method. After the ionic liquid is completely eluted, use deionized water as the eluent to elute The flow rate was 10 mL / min. The recovered ionic liquid was confirmed by infrared spectroscopy and nuclear magnetic resonance spectroscopy (see figure 2 and image 3 ), wherein the recovery rate of ionic liquid was 96.5%.

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Abstract

The invention discloses a method for in-situ enzymolysis and recovery of cellulose, which comprises the following steps of: dissolving cellulose at the temperature of between 40 and 120 DEG C by using ionic liquid, adding a solvent to form an ionic liquid / solvent system, and adding cellulase for in-situ enzymolysis of the cellulose at the temperature of between 40 and 60 DEG C, wherein the ionic liquid accounts for 5 to 100 percent of the total volume of the ionic liquid / solvent system, the ionic liquid is imidazole phosphate ionic liquid, the solvent is citric acid buffer or acetic acid-sodium acetate solution with the pH of 4.8, and the addition amount of the cellulase is 0.5-50 mg per milliliter of ionic liquid / solvent system; and recovering the ionic liquid and sugar by adopting an adsorptive separation method or aqueous two-phase separation method. The method has the advantage of simple process, and also has the advantages of high efficiency, low organic solvent consumption, economic process, less three wastes and the like compared with the conventional two-step method of processing the cellulose by the ionic liquid and performing regenerative enzymolysis.

Description

technical field [0001] The invention relates to a process and an enzymatic hydrolysis system for enzymolysis of cellulose in an ionic liquid / solvent system, and a method for recovering the ionic liquid after enzymatic hydrolysis. Background technique [0002] Cellulose has extremely strong hydrogen bonds between molecules and within molecules, which makes cellulose insoluble in common solvents and difficult to be used directly. Therefore, the dissolution of cellulose has always been a key problem to be solved in the process of comprehensive utilization of fiber resources. Traditionally, chemical modification methods (such as ketone ammonia complex method, xanthate method, etc.) are used to treat cellulose. This method has the disadvantages of high energy consumption, serious pollution, and large investment. In view of this, scientists from various countries are devoting themselves to the development of novel cellulose research solvents, mainly diazepam / dimethylformamide (N ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02C07F9/6506C07H3/02C07H1/06
CPCY02P20/54
Inventor 咸漠李良智何玉财谭伟强李强杨方
Owner 青岛生物能源与过程研究所