Semi-quantitative detection method for residue of bleaching and coloring agents in traditional Chinese medicinal materials and traditional Chinese medicinal decoction pieces

A technology of semi-quantitative detection and traditional Chinese medicine decoction pieces, which is applied in the field of physical and chemical analysis of chemical substances, can solve the problems of high processing conditions for the toxic reagent tetrachloroamalgam, expensive equipment, and high requirements for the detection environment, and achieve the elimination of complex matrix interference, The effect of eliminating color background noise and shortening the detection time

Inactive Publication Date: 2011-08-17
广东省药品检验所
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because this type of substance has the same strong reducing property as sulfur dioxide, it has good bleaching, anti-oxidation and anti-corrosion effects. Similarly, it also brings great hidden dangers to the safe use of Chinese medicinal materials and Chinese herbal medicines.
At present, the legal method to detect whether there is sulfur dioxide residue in Chinese herbal medicines and herbal pieces is the distillation titration method. This method uses a glass instrument device, which is large in size, has many connecting parts, and uses nitrogen gas. The operation time is too long and must be carried out in the laboratory. and some studies try to learn from the rose paraaniline colorimetric method or ion chromatography detection of sulfur dioxide in food, but either use the toxic reagent tetrachloro amalgam and the processing conditions are relatively high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Semi-quantitative detection method for residue of bleaching and coloring agents in traditional Chinese medicinal materials and traditional Chinese medicinal decoction pieces
  • Semi-quantitative detection method for residue of bleaching and coloring agents in traditional Chinese medicinal materials and traditional Chinese medicinal decoction pieces
  • Semi-quantitative detection method for residue of bleaching and coloring agents in traditional Chinese medicinal materials and traditional Chinese medicinal decoction pieces

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Utilize the method of the present invention to carry out the color reaction of standard substance

[0044] In this embodiment, sodium sulfite and sodium bisulfite are used as standard products. Take about 10 g of standard sodium sulfite and sodium bisulfite, put them in 100ml measuring bottles, add an appropriate amount of purified water or distilled water to dissolve and dilute to the mark, and use them as respective standard stock solutions.

[0045] Take the above stock solution and prepare the mass volume percentage concentration as 0.0001%, 0.0002%, 0.0005%, 0.001%, 0.002%, 0.005%, 0.01%, 0.02%, 0.05%, 0.1%, 0.2%, 0.5%, 1% , 2%, 5%, 10% and other standard solutions, and take 2ml respectively to carry out the color reaction according to the following steps:

[0046] (1) Sample processing

[0047] The sampling volume of Chinese herbal medicines and Chinese herbal medicine pieces is about 1g. Cut (or grind) the sample into fine particles with scissors...

Embodiment 2

[0053] Embodiment 2: Utilize the method of the present invention to carry out the color reaction of sample

[0054] First, prepare the test solution. Place the treated sample in a cecillin bottle filled with 10ml of 0.125% sodium hydroxide solution. The specification of the bottle is 15ml. Tighten the stopper, shake vigorously for 1-2 minutes, let stand for a while, and wait for the layers to separate. Take 2ml of the upper layer solution (filtered if necessary) as the test solution. A color reaction is then performed. Put 2ml of the above-mentioned test solution in a vial containing arsenic-free zinc pellets, wherein, the arsenic-free zinc pellets are 2-3, about 0.2g, the specification of the vial is 10ml, and then add 2ml of hydrochloric acid solution, Shake gently to make the mixture even, and immediately put the lead acetate test paper tightly on the mouth of the bottle. Final judgment result. If it turns yellow-brown to silver-black within 10 minutes, it can be judged...

Embodiment 3

[0055] Embodiment 3: the selection of filter paper

[0056] Select qualitative analysis filter paper, cut it into a circle with a diameter of about 2 cm (coinciding with the mouth of the vial), and soak it with lead acetate, copper acetate, copper sulfate and other test solutions respectively during the experiment before use. During the experiment, the above-mentioned prepared filter papers were respectively placed in the mouth of the cillin bottle for reaction, and the experimental results are shown in Figure 5 , Figure 5 After soaking the test paper with lead acetate, copper acetate and copper sulfate, it can detect 0.01%, 0.005%, 0.002%, 0.001%, 0.0005%, 0.0002%, 0.0001% Na 2 SO 3 and NaHSO 3 And graphs of experimental results with various samples. Depend on Figure 5 It can be seen that when the concentration is greater than 0.002%, both the sulfite standard solution and the sample can have a better color reaction. Therefore, according to the requirements of daily ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides a semi-quantitative detection method for residue of bleaching and coloring agents in traditional Chinese medicinal materials and traditional Chinese medicinal decoction pieces. The method comprises the following steps of: reacting the treated sample with solution of sodium hydroxide, and taking 2ml of the upper clarified solution as test solution; and adding the 2ml of test solution into a vial filled with arsenic-free zinc granules, adding 2ml of hydrochloric acid, uniformly mixing, and tightly adhering lead or copper ion test paper to the opening of the vial immediately; and if the solution turns yellowish-brown to silver black within ten minutes, preliminarily determining that the sample contains sulfite bleaching and coloring agents, wherein the lead or copper ion test paper is lead acetate test paper, copper acetate test paper or copper sulfate test paper. The method has the advantages that: the vial is convenient to carry, the method is easy to operate, low in cost and high in accuracy, the residual can be detected quickly, semi-quantitative measurement is adopted and the like; moreover, the method greatly shortens the detection time, and is applicable to supervising of rapid screening of the residue of bleaching and coloring agents in the traditional Chinese medicinal materials and the traditional Chinese medicinal decoction pieces on site and performing semi-quantitative analysis.

Description

technical field [0001] The invention relates to physical and chemical analysis of chemical substances, in particular to a semi-quantitative detection method for residual bleaching and coloring agents in Chinese medicinal materials and Chinese medicinal slices. Background technique [0002] Scientific studies have shown that long-term or high-dose intake of sulfite can cause damage to the gastrointestinal tract and liver, and sulfur dioxide can damage the reproductive system of male mice. Since the 1980s, research on the safety of sulfites has attracted more and more attention from various countries. [0003] The traditional processing methods of sulfur fumigation or soaking Chinese herbal medicines will lead to a large amount of sulfur dioxide, arsenic, mercury and other toxic and harmful substances remaining in the original medicine of Chinese herbal medicines. Therefore, "The Pharmacopoeia of the People's Republic of China" (2000 Edition) stipulates that, except for yam, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78
Inventor 林奇艺林锦锋严全鸿谢志洁
Owner 广东省药品检验所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap