Methods of using crop straws to prevent and control soil-borne diseases of vegetables
A technology for crop straws and soil-borne diseases is applied in the field of prevention and control of vegetable soil-borne diseases, which can solve the problems of large differences in field soil environment, and it is difficult to completely eliminate pathogenic bacteria, so as to reduce the pressure of environmental pollution and kill or inhibit pathogenic bacteria. , the effect of low input cost
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Embodiment 1
[0025] Effects of Rice Straw Flooding on Soil Physical and Chemical Properties
[0026] The test soil was taken from the hot pepper production greenhouse in Gaogou Town, Huaian, and the rice straw was taken from the rice field of Jiangsu Academy of Agricultural Sciences. The pathogen was Phytophthora capsici ( Phytophthoracapsici ), which can be obtained from the Agricultural Microorganism Culture Collection Center of the Chinese Academy of Agricultural Sciences. The pepper variety tested was Luojiao No. 5. The spore liquid of Phytophthora capsici was evenly added to the test soil, so that the concentration of Phytophthora spores in the soil was 200 Phytophthora spores / gram of dry soil. Three treatments were set up in the experiment: (1) Control (CK): After the soil was inoculated with Phytophthora capsici, the soil moisture content was kept at 16-18%, and it was placed naturally. (2) Conventional flooding (flooding, F): the soil is inoculated with Phytophthora capsici and ...
Embodiment 2
[0033] Embodiment 2 The impact of rice straw flooding on soil cellulase activity
[0034] Collect the soil after flooding and drying in Example 1, and measure the activity of soil cellulase.
[0035] It can be seen from Table 1 that the soil cellulase activity of rice straw flooded soil was higher than that of the control and conventional flooded. Soil enzyme activity is an important indicator of soil biological properties. Generally, healthy soil has higher soil cellulase activity. In addition, the main component of the cell wall of Phytophthora capsici is cellulose, and the increase of cellulase activity in the soil can inhibit the growth of Phytophthora capsici or degrade the pathogen, reducing the probability of occurrence of Phytophthora capsici.
[0036] Table 1 Effect of rice straw flooding on soil cellulase activity
[0037]
[0038] Note: The unit of soil cellulase activity is mg glucose / (10g·72h).
Embodiment 3
[0039] Embodiment 3: Inhibitory effect of rice straw flooding on the growth of Phytophthora capsici in soil
[0040] The amount of Phytophthora capsici in each treatment was determined by quantitative PCR (RT-PCR) method on the soil treated by flooding in Example 1. Determination method of Phytophthora capsici: the kit used for soil DNA extraction is FastDNASPINKitforSoil produced by MP Company, and the soil DNA was extracted according to the instructions. The specific primers are CAPFW (5'-TTTAGTTGGGGGTCTTGTACC-3') and CAPRV1 (5'-CCTCCACAACCAGCAACA-3'), and the target fragment size is about 450bp. The RT-PCR reaction kit used SYBRpremixExTaq from TaKaRa Company. The fluorescent quantitative PCR amplification system was: 2×SYBRPreMixExTaq (TaKaRa) 10 μL, 50×ROXReferenceDye II (TaKaRa) 0.4 μL, 20 μmol / L primers 0.4 μL each, template 2 μL, ddH2O 6.8 μL, and the amplification system was 20 μL. The reaction program is: 95°C, 30s, 1 cycle; 95°C, 5s, 60°C, 34s, 40 cycles. Fluores...
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