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Methods of using crop straws to prevent and control soil-borne diseases of vegetables

A technology for crop straws and soil-borne diseases is applied in the field of prevention and control of vegetable soil-borne diseases, which can solve the problems of large differences in field soil environment, and it is difficult to completely eliminate pathogenic bacteria, so as to reduce the pressure of environmental pollution and kill or inhibit pathogenic bacteria. , the effect of low input cost

Active Publication Date: 2016-01-20
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many studies have also shown that it is difficult to completely eliminate pathogenic bacteria by flooding alone
[0008] There are many reports at home and abroad about the growth inhibition and even killing effects of straw decomposition products on various soil-borne pathogens. It is believed that organic acids and phenols produced by straw degradation Allelochemicals such as acids will directly kill or inhibit pathogenic bacteria; at the same time, other decomposition products of straw can also provide rich nutrient sources for soil microorganisms (especially bacteria) that are not sensitive to allelochemicals, significantly increasing the number and diversity of soil microorganisms Enhance soil bioactivity, improve nutrient utilization, promote crop growth and enhance disease resistance, but these reports are under indoor simulated dry farming conditions, using straw mulch or evenly mixing straw into the soil to keep moisture and cultivate for a certain period of time Finally, the straw decomposed liquid was collected to measure the growth inhibitory effect on the target pathogenic bacteria in the state of in vitro and pure culture, which was quite different from the actual field soil environment.
However, the pathogenic bacteria were directly inoculated into the soil containing straw, and the in situ research on the inhibitory effect of straw decomposition products on the pathogenic bacteria in the soil and the effect on the control of soil-borne diseases of vegetables under continuous flooding conditions has not been reported so far.

Method used

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  • Methods of using crop straws to prevent and control soil-borne diseases of vegetables
  • Methods of using crop straws to prevent and control soil-borne diseases of vegetables

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Effects of Rice Straw Flooding on Soil Physical and Chemical Properties

[0026] The test soil was taken from the hot pepper production greenhouse in Gaogou Town, Huaian, and the rice straw was taken from the rice field of Jiangsu Academy of Agricultural Sciences. The pathogen was Phytophthora capsici ( Phytophthoracapsici ), which can be obtained from the Agricultural Microorganism Culture Collection Center of the Chinese Academy of Agricultural Sciences. The pepper variety tested was Luojiao No. 5. The spore liquid of Phytophthora capsici was evenly added to the test soil, so that the concentration of Phytophthora spores in the soil was 200 Phytophthora spores / gram of dry soil. Three treatments were set up in the experiment: (1) Control (CK): After the soil was inoculated with Phytophthora capsici, the soil moisture content was kept at 16-18%, and it was placed naturally. (2) Conventional flooding (flooding, F): the soil is inoculated with Phytophthora capsici and ...

Embodiment 2

[0033] Embodiment 2 The impact of rice straw flooding on soil cellulase activity

[0034] Collect the soil after flooding and drying in Example 1, and measure the activity of soil cellulase.

[0035] It can be seen from Table 1 that the soil cellulase activity of rice straw flooded soil was higher than that of the control and conventional flooded. Soil enzyme activity is an important indicator of soil biological properties. Generally, healthy soil has higher soil cellulase activity. In addition, the main component of the cell wall of Phytophthora capsici is cellulose, and the increase of cellulase activity in the soil can inhibit the growth of Phytophthora capsici or degrade the pathogen, reducing the probability of occurrence of Phytophthora capsici.

[0036] Table 1 Effect of rice straw flooding on soil cellulase activity

[0037]

[0038] Note: The unit of soil cellulase activity is mg glucose / (10g·72h).

Embodiment 3

[0039] Embodiment 3: Inhibitory effect of rice straw flooding on the growth of Phytophthora capsici in soil

[0040] The amount of Phytophthora capsici in each treatment was determined by quantitative PCR (RT-PCR) method on the soil treated by flooding in Example 1. Determination method of Phytophthora capsici: the kit used for soil DNA extraction is FastDNASPINKitforSoil produced by MP Company, and the soil DNA was extracted according to the instructions. The specific primers are CAPFW (5'-TTTAGTTGGGGGTCTTGTACC-3') and CAPRV1 (5'-CCTCCACAACCAGCAACA-3'), and the target fragment size is about 450bp. The RT-PCR reaction kit used SYBRpremixExTaq from TaKaRa Company. The fluorescent quantitative PCR amplification system was: 2×SYBRPreMixExTaq (TaKaRa) 10 μL, 50×ROXReferenceDye II (TaKaRa) 0.4 μL, 20 μmol / L primers 0.4 μL each, template 2 μL, ddH2O 6.8 μL, and the amplification system was 20 μL. The reaction program is: 95°C, 30s, 1 cycle; 95°C, 5s, 60°C, 34s, 40 cycles. Fluores...

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Abstract

The invention relates to a method for preventing and controlling soil-borne diseases of vegetables by using crop stalks, which is characterized in that the soil is flooded after the crop stalks are applied to the field, and the specific steps are as follows: when the soil temperature is above 20°C, the Clean up the residues of previous crops, surround the field with soil around a ridge about 20 cm high, spread the crop stalks crushed to a length of 10-15 cm evenly on the field, and then plow the soil to a depth of 18-20 cm , use irrigation water to flood the field for 5 days. During the flooding period, the field water depth is kept at 5 cm above the surface of the soil layer. After 5 days, the soil is rotated to a depth of 18-20 cm. When the reduction potential drops to 0-40mv, the flooding ends. After the soil is naturally dried, chemical fertilizers are applied, and then the soil is rotated to plant crops. The application amount of chemical fertilizers is: 30-40 kg of urea and 20-30 kg of potassium phosphate fertilizer .

Description

technical field [0001] The invention relates to a method for preventing and controlling soil-borne diseases of vegetables by using crop stalks. Specifically, the crop stalks are properly crushed and evenly spread to the field, the soil is plowed and flooded for a certain period of time, and then the water is drained, and the soil is natural. Planting vegetables after drying can achieve the effect of preventing and controlling soil-borne diseases of vegetables. The invention belongs to the field of comprehensive prevention and control of vegetable diseases by using agricultural waste. Background technique [0002] my country is a large agricultural country with large output, wide distribution and variety of crop straw, which has been a valuable resource for farmers' life and agricultural development for a long time. In recent years, with the rapid development of crop breeding technology, the continuous updating of high-yield crop varieties and the continuous improvement of a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01B79/02
Inventor 马艳王光飞顾志光徐跃定张建英
Owner JIANGSU ACAD OF AGRI SCI
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