Method for increasing microbe conversion rate of bagasse by utilizing non-ionic surface active agent
A non-ionic surface and surfactant technology, applied in the application field of microbial fermentation and transformation, can solve the problems of low bio-saccharification efficiency, high substrate degradation rate, low raw material cost, etc., to reduce investment costs and production costs, reduce The effect of bacterial contamination and production cost saving
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Embodiment 1
[0040] Embodiment 1: Utilize non-ionic surfactant to improve the method of bagasse microbial conversion effect
[0041](1) Preparation of the seed solution of Clostridium thermocellum (ATCC27405): The Clostridium thermocellum stored at -80°C was activated in a 10mL vial and amplified in a 100mL serum bottle, and cultured for 18 hours to obtain OD600 ~0.6 seed solution; the medium is MTC medium, the main components of the medium are A, B, C, D, and E liquid, and the five components are used according to 45:2:1:1:1 (V / V). Bacteria syringe mix. Liquid A is 3g / L cellulose; Liquid B: tripotassium citrate 50g / L, citric acid monohydrate 31.25g / L, sodium sulfate 25g / L, potassium dihydrogen phosphate 25g / L, sodium bicarbonate 62.5g / L; liquid C: ammonium chloride 75g / L, urea 250g / L, yeast extract 50g / L; liquid D: magnesium chloride hexahydrate 50g / L, ferrous chloride tetrahydrate 5g / L, chloride dihydrate Calcium 10g / L, cysteine hydrochloride monohydrate 50g / L; liquid E: pyridoxamin...
Embodiment 2
[0045] Embodiment 2: Utilize nonionic surfactant to improve the method of bagasse microbial conversion effect
[0046] (1) Preparation of the seed solution of Clostridium thermocellum (ATCC27405): The Clostridium thermocellum stored at -80°C was activated in a 10mL vial and amplified in a 100mL serum bottle, and cultured for 18 hours to obtain OD600 ~0.6 seed solution; the medium is MTC medium, the main components of the medium are A, B, C, D, and E liquid, and the five components are used according to 45:2:1:1:1 (V / V). Bacteria syringe mix. Liquid A is 3g / L cellulose; Liquid B: tripotassium citrate 50g / L, citric acid monohydrate 31.25g / L, sodium sulfate 25g / L, potassium dihydrogen phosphate 25g / L, sodium bicarbonate 62.5g / L; liquid C: ammonium chloride 75g / L, urea 250g / L, yeast extract 50g / L; liquid D: magnesium chloride hexahydrate 50g / L, ferrous chloride tetrahydrate 5g / L, chloride dihydrate Calcium 10g / L, cysteine hydrochloride monohydrate 50g / L; liquid E: pyridoxamin...
Embodiment 3
[0049] Embodiment 3: Utilize non-ionic surfactant to improve the method of bagasse microbial conversion effect
[0050] (1) The preparation of the seed solution is the same as in Example 1.
[0051] (2) Preparation of fermentation medium, preparation and sterilization of surfactant solution: add 10g / L bagasse as carbon source to the fermentation medium instead of liquid A, other ingredients and dosage ratio are the same as MTC medium; prepare 125g / L of Triton X-100 aqueous solution; seal the serum bottle containing the fermentation medium and the serum bottle containing the surfactant with a rubber stopper and an aluminum cap, and repeat vacuuming and filling with 0.01MPa nitrogen gas for 3 times, and finally at 115°C Sterilize for 20 minutes;
[0052] (3) Microbial transformation and hydrogen-producing fermentation of bagasse: Inject the prepared seed liquid into the sterilized fermentation medium at an inoculation amount of 10v / v%, and perform anaerobic thermophilic fermen...
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