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Preparation method for regioselective biological-contamination resistant array chip

An anti-biological pollution, array chip technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, biological testing, etc., can solve the problems of sample point diffusion, difficult control of array size, etc., achieve low equipment requirements, reduce non-special Effect of heterosexual adsorption and simple process

Inactive Publication Date: 2014-08-06
HARBIN INST OF TECH AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing array processing methods mostly rely on complex instruments and equipment, such as robot hand sample spotting devices, which are prone to cause problems such as sample point diffusion and difficult control of array size.

Method used

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  • Preparation method for regioselective biological-contamination resistant array chip
  • Preparation method for regioselective biological-contamination resistant array chip
  • Preparation method for regioselective biological-contamination resistant array chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] Example 1: A method for preparing a regioselective anti-biological contamination array chip

[0119] Will pass through Piranha solution (H 2 SO 4 / H 2 o 2 =4 / 1, v / v) After cleaning, place the 1cm×1cm silicon chip in 2% (v / v) methanol solution of 5-hexenyltrimethoxysilane, react at 25°C for 30 minutes, wash with methanol Clean and dry. The structure of 5-hexenyltrimethoxysilane is as follows:

[0120]

[0121] 40g / L methanol solution of 3-[[2-(methacryloyloxy)ethyl](dimethyl)-ammonium]-1-propanesulfonic acid (SBMA) betaine Spin-coat on the chip surface at a spin-coating speed of 2000 rpm for 10 seconds. The structure of SBMA betaine is:

[0122]

[0123]Spread a mask with an array pattern on the surface of the chip and transfer to 20mW / cm 2 and 365nm ultraviolet light for 3 minutes to initiate graft polymerization of SBMA betaine and the surface of the chip to form non-array regions. After washing with 50% aqueous methanol solution, 40g / L of anti-biofoulin...

Embodiment 2

[0126] Example 2: A method for preparing a regioselective anti-biological contamination array chip

[0127] Place a 1cm×1cm polydimethylsiloxane sheet in a 5% (v / v) ethanol solution of 3-(methacryloyloxy)propyltrimethoxysilane, react at 25°C for 2 hours, and use Wash with 50% aqueous methanol and dry. The structure of 3-(methacryloyloxy)propyltrimethoxysilane is:

[0128]

[0129] Spin-coat the methanol / water (methanol / water=1 / 1, v / v) solution of 30g / L anti-biological contamination monomer—glyceryl methacrylate monomer on the surface of the chip, the spin-coating speed is 1500 rpm, and the time is 60 seconds. The structure of glyceryl methacrylate is:

[0130]

[0131] Spread a mask with an array pattern on the surface of the chip and transfer to 20mW / cm 2 1. Under 365nm ultraviolet light, the ultraviolet light is irradiated on the surface of the chip through the mask for 5 minutes to trigger the graft polymerization of glycerol methacrylate and the surface of the ch...

Embodiment 3

[0134] Example 3: A method for preparing a regioselective anti-biological contamination array chip

[0135] Will pass through Piranha solution (H 2 SO 4 / H 2 o 2 =4 / 1, v / v) Cleaned 1cm×1cm glass slides were placed in 2% (v / v) 3-aminopropyltriethoxysilane toluene solution, reacted at 70°C for 5 hours, and washed with toluene Wash and dry under vacuum at 120°C for 1 hour. The structure of 3-aminopropyltriethoxysilane is:

[0136]

[0137] The chips were dipped in 10% (v / v) glycidyl methacrylate in acetone solution, reacted at 25°C for 8 hours, washed with acetone and dried. The structure of glycidyl methacrylate is:

[0138]

[0139] A methanol solution of 4% (v / v) anti-biofouling monomer-hydroxyethyl methacrylate was spin-coated on the chip surface at a spin-coating speed of 2500 rpm for 10 seconds. The structure of hydroxyethyl methacrylate is:

[0140]

[0141] Spread a mask with an array pattern on the surface of the chip and transfer to 15mW / cm 2 1. Under ...

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Abstract

The invention discloses a processing method, based on a matrix of glass, silicon chips and dimethyl silicone polymer, for a biological-contamination resistant array chip. According to the invention, different biological-contamination resistant materials are adopted to build an array area and a non-array area of the chip respectively, so that the surfaces of the entire chip all have the biological-contamination resistant function, and the array area can react with biological components to obtain a functionalized biological probe array. The processing method comprises the following specific steps: conducting silanization on the surfaces of matrix materials; conducting graft polymerization on a biological-contamination resistant monomer; conducting graft polymerization on a biological-contamination resistant and functionalized monomer; conducting the coupling reaction between the biological components and the array area. The array biological chip prepared from the processing method is greatly low in biomolecular nonspecific adsorption, high in probe molecular density and high in signal sensitivity.

Description

technical field [0001] The invention relates to a preparation method of an anti-biological pollution array chip, belonging to the technical field of biological chips. Background technique [0002] The essence of biochip technology is to build an ordered lattice on the substrate, fix the probe molecules, make them bind or react with the tested substance, and then obtain the molecular structure and concentration information of the tested substance through detection and analysis. This technology has the advantages of high throughput, high information content, miniaturization, automation, and rapid response, and can be applied in clinical diagnosis, environmental monitoring, food safety and other fields. But so far, biochips, especially protein chips, have not been widely used in clinical testing. The main reason is that clinical samples, such as blood and urine samples, contain a large number of biomacromolecules, which are easy to be non-specifically adsorbed on the surface o...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68C40B50/14
CPCC12Q1/6837G01N33/68C12Q2565/501
Inventor 孙秀花王怀新高昌录
Owner HARBIN INST OF TECH AT WEIHAI
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