Oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and preparation method thereof

A technology of oxidizing chitosan and grafting modification, which is applied in fiber treatment, medical science, absorbent pads, etc., can solve the problems of inconvenient large-scale promotion and use, expensive cross-linking agent, insufficient mechanical properties, etc. Large-scale promotion and use, high chemical reactivity, and excellent comprehensive performance

Active Publication Date: 2014-09-03
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to improve the mechanical properties of collagen-based materials in the in vivo environment, such as insufficient mechanical properties, too fast degradation rate, and insufficient stability, it is necessary to use a certain cross-linking agent. Linked, genipin cross-linked, epoxy compounds, etc., although the enzymolysis resistance has been greatly improved after being modified by each cross-linking agent, these cross-linking age

Method used

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  • Oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and preparation method thereof
  • Oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and preparation method thereof
  • Oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and preparation method thereof

Examples

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Embodiment 1

[0033] (1) Preparation of purified pig dermis: Remove the subcutaneous layer of fresh ecological pig skin on a meat remover, put the pig skin into the drum, and use 10% flattened at 25 °C to repeatedly degrease 3 times, and then freeze and thaw repeatedly for 3 times. For the second time, use 5mM Tris, 5mM EDTA and 0.1M NaCl to remove impurities and decellularize, use trypsin with an activity unit of 1:10 to perform decellularization again, and wash repeatedly with deionized water , and finally dried by a freeze dryer to obtain purified pig dermis;

[0034] (2) Preparation of biological cross-linking agent—oxidized chitosan: Pour 1000 parts by volume of acetic acid-sodium acetate buffer solution with a pH of 3.0 into the reaction kettle, and another 10 parts by weight of deacetylation degree of 75% Chitosan was also added to the reaction kettle, stirred at room temperature until clarified, then slowly added 1 weight part of sodium periodate, stirred and reacted at 4°C for 24 h...

Embodiment 2

[0039] (1) Preparation of purified pig dermis: Remove the subcutaneous layer of fresh ecological pig skin on a meat remover, put the pig skin into the drum, and use 12% flat to degrease repeatedly at 35°C for 3 times, and then freeze and thaw repeatedly for 3 times. Second, use 20mM Tris, 10mM EDTA and 0.5M NaCl to remove impurities and decellularize, use trypsin with an activity unit of 1:20 to perform decellularization again, and wash repeatedly with deionized water , and finally dried by a freeze dryer to obtain purified pig dermis;

[0040] (2) Preparation of biological cross-linking agent—oxidized chitosan: Pour 1500 parts by volume of acetic acid-sodium acetate buffer solution with a pH of 3.0 into the reaction kettle, and another 10 parts by weight of deacetylation degree of 85% Chitosan was also added to the reaction kettle, stirred at room temperature until clarified, then slowly added 10 parts by weight of sodium periodate, stirred and reacted at 6°C for 48 hours, ad...

Embodiment 3

[0045] (1) Preparation of purified pig dermis: Remove the subcutaneous layer of fresh ecological pig skin on a meat remover, put the pig skin into the drum, and use 15% flattened at 35 °C to repeatedly degrease 3 times, and then freeze and thaw repeatedly for 3 times. Next, use 40mM Tris, 15mM EDTA and 1.0M NaCl to remove impurities and decellularize, use trypsin with an activity unit of 1:25 to perform decellularization again, and wash repeatedly with deionized water , and finally dried by a freeze dryer to obtain purified pig dermis;

[0046] (2) Preparation of biological cross-linking agent—oxidized chitosan: Pour 2000 parts by volume of acetic acid-sodium acetate buffer solution with a pH of 3.0 into the reaction kettle, and another 10 parts by weight of deacetylation degree of 95% Chitosan was also added to the reaction kettle, stirred at room temperature until clarified, then slowly added 25 parts by weight of sodium periodate, stirred and reacted at 6°C for 60 hours, ad...

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Abstract

The invention discloses an oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and a preparation method thereof. The oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane is characterized in that the preparation method comprises the steps: by applying an electrostatic spinning technology and using traceable purified porcine dermis as a raw material and hexafluoroisopropanol as a solvent, stirring at 4-10 DEG C until the mixture is transparent, formulating to obtain electrostatic spinning mother liquor with concentration of 0.5-10%, and directly spinning to obtain the porcine dermal collagen micro-nano fiber membrane; then performing graft modification on the porcine dermal collagen micro-nano fiber membrane by using oxidation chitosan, thereby finally obtaining the oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane. A membrane material not only has good bio-compatibility, biodegradability and mechanical properties, but also has anti-oxidation effects, antibacterial activities, anti-inflammation/wound healing promotion effects, anti-cancer/anti-tumor effects and antiviral effects of chitosan, is significantly better than a collagen composite membrane material prepared by using collagen as the raw material, and can be widely applied to the preparation of biomedical materials such as hemostatic materials, tissue engineering scaffold materials, biological dressings and the like.

Description

technical field [0001] The invention relates to an oxidized chitosan graft modified porcine dermis collagen micro-nano fiber membrane and a preparation method thereof, belonging to the field of preparation of biomedical materials. Background technique [0002] In recent years, electrospinning technology is often used to prepare nano-scale continuous ultrafine fibers, because its structure can maximize the bionic extracellular matrix (Li Huan. Research progress in electrospinning of gelatin and collagen[J]. Gelatin Science and Technology, 2007, 27(1):1-9), so this kind of scaffold is widely used in the preparation of tissue engineering scaffold materials, biological dressings, etc. However, hexafluoroisopropanol or trifluoroacetic acid is usually used as a solvent for collagen electrospinning, and the fiber membrane obtained by electrospinning has poor mechanical properties and is easily dispersed in water. , Andrew K. E., Yen W. T., Lin Y .L. Y., et al. Electro-spinning of ...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/54A61L15/32A61L15/44D06M15/03
Inventor 但卫华刘新华但年华刘婷
Owner SICHUAN UNIV
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