Oxidized chitosan grafted modified porcine dermis collagen micro-nanofibrous membrane and preparation method thereof

Abstract

The invention discloses an oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and a preparation method thereof. The oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane is characterized in that the preparation method comprises the steps: by applying an electrostatic spinning technology and using traceable purified porcine dermis as a raw material and hexafluoroisopropanol as a solvent, stirring at 4-10 DEG C until the mixture is transparent, formulating to obtain electrostatic spinning mother liquor with concentration of 0.5-10%, and directly spinning to obtain the porcine dermal collagen micro-nano fiber membrane; then performing graft modification on the porcine dermal collagen micro-nano fiber membrane by using oxidation chitosan, thereby finally obtaining the oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane. A membrane material not only has good bio-compatibility, biodegradability and mechanical properties, but also has anti-oxidation effects, antibacterial activities, anti-inflammation / wound healing promotion effects, anti-cancer / anti-tumor effects and antiviral effects of chitosan, is significantly better than a collagen composite membrane material prepared by using collagen as the raw material, and can be widely applied to the preparation of biomedical materials such as hemostatic materials, tissue engineering scaffold materials, biological dressings and the like.

Description

technical field [0001] The invention relates to an oxidized chitosan graft modified porcine dermis collagen micro-nano fiber membrane and a preparation method thereof, belonging to the field of preparation of biomedical materials. Background technique [0002] In recent years, electrospinning technology is often used to prepare nano-scale continuous ultrafine fibers, because its structure can maximize the bionic extracellular matrix (Li Huan. Research progress in electrospinning of gelatin and collagen[J]. Gelatin Science and Technology, 2007, 27(1): 1-9), so this type of scaffold is widely used in the preparation of tissue engineering scaffold materials, biological dressings, etc. However, usually hexafluoroisopropanol or trifluoroacetic acid is used as a solvent for collagen electrospinning, and the fiber membrane obtained by electrospinning has poor mechanical properties and is easily dispersed in water. .,ElijahS.Y.Y.,AndrewK.E.,YenW.T.,LinY.L.Y.,etal.Electro-spinningof...

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Problems solved by technology

[0004] In order to improve the mechanical properties of collagen-based materials in the in vivo environment, such as insufficient mechanical properties, too fast degradation rate, and insufficient stability, it is necessary to use a certain cross-linking agent. Linked, genipin cross-linked, epoxy compounds, etc., although the enzymolysis resistance has been greatly improved after being modified by each cross-linking agent, these cross-linking agents exist more or less, such as potential biological toxicity, mechanical properties And the control of enzymolysis resistance performance all shows certain limitation, and some cross-linking agents are expensive, are not easy to popularize and use on a large scale (HuY., LiuL., GuZ., DanW., DanN., & YuX.Modificationofcollagenwithnaturalderivedcross- linker, alginated aldehyde [J]. Carbohydrate Polymers, 2014, 102(15): 324–332)

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