Ultrasound lipid microbubble wrapping drug-carrying albumin nanoparticles and preparation method thereof

A technology of albumin nanoparticles and lipid microbubbles, applied in liposome delivery, echo/ultrasound imaging agents, microcapsules, etc., can solve the problem of low targeting efficiency of nanoparticles, low drug encapsulation efficiency and low drug loading capacity and other problems, to achieve the effect of improving the efficiency of targeted drug delivery, increasing the drug loading capacity, and simplifying the preparation process

Inactive Publication Date: 2014-10-15
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of this method is to solve the problems of low drug encapsulation efficiency and drug loading capacity of lipid microbubbles and low targeting efficiency of nanoparticles, and provide a new lipid microbubble that simultaneously encapsulates drug-loaded albumin nanoparticles and gas preparation method

Method used

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  • Ultrasound lipid microbubble wrapping drug-carrying albumin nanoparticles and preparation method thereof
  • Ultrasound lipid microbubble wrapping drug-carrying albumin nanoparticles and preparation method thereof
  • Ultrasound lipid microbubble wrapping drug-carrying albumin nanoparticles and preparation method thereof

Examples

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Embodiment 1

[0022] 1. Preparation of lecithin microbubbles loaded with fluorocarbon gas

[0023] The lecithin, glycerol and phosphate buffer are mixed and heated, and the mass volume ratio of glycerol to phosphate buffer is 58.1%. The mass volume ratio of lecithin to phosphate buffer is 6.1%. Fill with fluorocarbon gas and shake for 40-50s to prepare blank lipid microbubbles.

[0024] 2. Preparation of curcumin albumin nanoparticles based on Nanoparticle albumin-bound technology

[0025] Precisely weigh the prescribed amount of curcumin and dissolve it in 1ml of ethyl acetate to form an oil phase, and another 40mg of albumin is dissolved in 4mL of double-distilled water to form an aqueous phase. Mix the two, stir with a magnetic stirrer for 5min to form colostrum, and store in an ice bath. The colostrum was placed in a cell pulverizer probe and ultrasonically (85W, 1min) to obtain an emulsion, and the emulsion was transferred to a rotary evaporator to remove ethyl acetate by rotary evap...

Embodiment 2

[0029] 1. Preparation of lecithin microbubbles loaded with fluorocarbon gas

[0030] The lecithin, glycerol and phosphate buffer are mixed and heated, and the mass volume ratio of glycerol to phosphate buffer is 58.1%. The mass volume ratio of lecithin to phosphate buffer is 6.1%. Fill with fluorocarbon gas and shake for 40-50s to prepare blank lipid microbubbles.

[0031] 2. Preparation of curcumin albumin nanoparticles

[0032] Prepare curcumin albumin nanoparticles by desolvation method, accurately weigh 10mg bovine serum albumin and dissolve it in 1mL water, and then weigh 4mg curcumin and dissolve it in 6mL absolute ethanol, and dissolve curcumin at a volume flow rate of 1mL / min Add the ethanol solution dropwise to the albumin aqueous solution, add a certain amount of 0.25% glutaraldehyde, stir in the dark for 4 hours to solidify, remove the ethanol by rotary evaporation at 35°C, and obtain curcumin albumin nanoparticle suspension. Its encapsulation efficiency was meas...

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Abstract

The invention discloses an ultrasound lipid microbubble wrapping drug-carrying albumin nanoparticles and a preparation method thereof. The drug-carrying albumin nanoparticles comprise drugs and albumin. An ultrasound lipid microbubble membrane material mainly comprises phospholipid components and can wrap gas and the drug-carrying albumin nanoparticles simultaneously to form a composite structure with the gas and the nanoparticles in the middle. According to the ultrasound lipid microbubble wrapping the drug-carrying albumin nanoparticles and the preparation method thereof, the drug-carrying capacity of the microbubble is high, the preparation technology is simple, the production cost is low, and the ultrasound lipid microbubble is prone to industrial production, not only can be taken as an ultrasound developer for disease diagnosis, but also can be taken as a drug-carrying preparation for disease treatment.

Description

【Technical field】 [0001] This paper relates to a lipid microbubble with drug-loaded albumin nanoparticles and gas wrapped in the middle and a preparation method thereof. 【Background technique】 [0002] With the development of ultrasound imaging technology, it was found that microbubbles can significantly improve the contrast quality of ultrasound images, thus developing ultrasound microbubbles with gas in the middle as ultrasound contrast agents. Ultrasound contrast agents can enhance the two-dimensional ultrasound imaging and blood flow Doppler effect of myocardial, liver, brain and other solid organs, and can significantly improve the ability of ultrasound to distinguish the shape of the lesion. [0003] Nano-drug carrier is a hotspot in the research of pharmaceutical preparations. Nanoparticles have the following advantages in drug delivery: relatively high drug loading and encapsulation efficiency, which can increase the blood drug concentration of the target, prolong t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K47/42A61K9/127A61K9/51
Inventor 张良珂王家玉贾运涛
Owner CHONGQING MEDICAL UNIVERSITY
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