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Polymer-brush-modified magnetic composite microsphere as well as preparation method and application thereof

A technology of magnetic composite microspheres and polymer brushes, applied in the field of biomaterials, can solve the problems of rough separation, limited separation effect, and poor target-specific binding ability, and achieve narrow particle size distribution, reduce magnetic shielding effect, and improve The effect of the ability to specifically bind

Active Publication Date: 2014-11-19
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, this type of composite microspheres still has shortcomings. Due to the limited binding sites on the surface of superparamagnetic nanoparticles and the poor ability to specifically bind to the target, the rough separation mainly depends on adsorption. Its separation effect is very limited

Method used

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  • Polymer-brush-modified magnetic composite microsphere as well as preparation method and application thereof
  • Polymer-brush-modified magnetic composite microsphere as well as preparation method and application thereof
  • Polymer-brush-modified magnetic composite microsphere as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Superparamagnetic Fe 3 o 4 Preparation of nanoparticles

[0048] Preparation of Fe by Polyol Method 3 o 4 Nanoparticles: Put 720 mg of iron acetylacetonate in a 100 mL three-neck flask with a condenser, vacuumize and blow nitrogen three times, and then inject 40 mL of triethylene glycol into the reaction system. Magnetic stirring, heating in a sand bath, slowly raising the temperature to 180°C, and keeping it warm for 30 minutes; then rapidly raising the temperature to 278°C (the boiling point of triethylene glycol), and refluxing for 30 minutes to obtain a black magnetic fluid (ie, Fe 3 o 4 original reaction solution of nanoparticles). Cool to room temperature, wash with ethanol : ethyl acetate (V1 : V2=1 : 10) mixed solution repeatedly three times, magnetically separate, and redisperse the product in ethanol, store at low temperature.

[0049] The obtained product has good superparamagnetism, the average particle size is 4-20nm, and the specific satu...

Embodiment 2

[0051] Example 2 Superparamagnetic Composite Microspheres P(St-AA) / Fe 3 o 4 preparation of

[0052] The polymer template is prepared by a soap-free emulsion polymerization method, as follows: a three-necked bottle, mechanical stirring, vacuuming and nitrogen flow are repeated three times to ensure that the reaction system is anhydrous and oxygen-free. Styrene monomer (St, 0.5 g) and distilled water (45 mL) were injected into the reaction system, emulsified at 350 rpm for 30 min under nitrogen protection. Heat the oil bath to 70°C, add a persulfate (such as ammonium persulfate APS) aqueous solution as an initiator (APS content is 3.3 mg), and react for 3 h. Then, styrene (4.5 g), acrylic acid (1.67 g), and water (5 mL) were injected into the reaction system respectively, and the reaction was continued for 5 h. After the reaction, a white emulsion was formed. After cooling to room temperature, the reaction solution was distilled under reduced pressure to remove unreacted m...

Embodiment 3

[0057] Example 3 Preparation of Superparamagnetic Composite Microspheres

[0058] Take the three-necked bottle, stir it mechanically, assemble the reaction device, and repeat three times of vacuuming and blowing nitrogen to ensure that the reaction system is anhydrous and oxygen-free. Styrene monomer (St, 0.5 g) and distilled water (45 mL) were injected into the reaction system, emulsified at 400 rpm for 40 min under nitrogen protection. Heat the oil bath to 70 ℃, add persulfate (such as sodium persulfate) aqueous solution as the initiator (sodium persulfate content is 3 mg), reaction: ethyl acetate (V1 : V2 = 1 : 10) mixed solution repeatedly washed three times , magnetic separation, and the resulting product was redispersed in ethanol and stored at low temperature.

[0059] The obtained product has good superparamagnetism, the average particle size is 4-20nm, and the specific saturation magnetization is higher than 50 emu / g.

[0060] High temperature method (see JACS 200...

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Abstract

The invention provides a polymer-brush-modified magnetic composite microsphere as well as a preparation method and application thereof. The polymer-brush-modified magnetic composite microsphere comprises a polymer core, a super-paramagnetic nanoparticle shell and a polymer brush in sequence from inside to outside, wherein the polymer brush is directly grafted onto the super-paramagnetic nanoparticle shell through atom transfer radical polymerization by using a carboxyl-containing water-soluble unsaturated monomer. By grafting the polymer brush onto a magnetic nanoparticle surface, the specific surface area and the protein binding site of the microsphere can be increased greatly, and the probability of the contact of a protein with a material is increased. Moreover, a specific functional group can be further grafted onto the polymer brush conveniently, so that the specific binding ability of the microsphere with a separation object is improved, and the aim of finer separation is fulfilled. The preparation method is simple, and is convenient to promote and apply; and the stability, operability and environmental friendliness of a reaction system are improved.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to a magnetic composite microsphere modified by a polymer brush, a preparation method and application thereof. technical background [0002] Superparamagnetic nanoparticles are widely used in magnetic resonance imaging, biological separation (cell separation, protein separation) etc.), drug delivery systems, magnetic hyperthermia and gene therapy and other biomedical aspects. Due to the small particle size of superparamagnetic nanoparticles, when used for biological separation, the magnetic force generated under the action of a magnetic field is also very small, and it needs to be under a strong magnetic field or for a long time to achieve a good separation effect. . At present, some researchers make composite microspheres by mixing superparamagnetic nanoparticles with polymers ( J. Polym. Sci., Part A: Polym. Chem. 2008, 46 ,3900-3910), ( J. Mater. Chem. 2006, 16 , 4480-4...

Claims

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Application Information

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IPC IPC(8): C08L51/10C08L25/08C08F292/00C08F212/08C08F220/06C08F2/44C08K3/22
Inventor 吴尧解丽芹蓝芳马少华顾忠伟
Owner SICHUAN UNIV
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