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High-purity natural collagenous fiber and preparation method thereof

A collagen fiber, high-purity technology, applied in the field of high-purity natural collagen fiber and its preparation, can solve the problems of poor mechanical and mechanical properties, low structural stability, poor biodegradability, etc., and achieves reduced degreasing time and narrow natural aggregation degree distribution. , Easy-to-clean effect

Active Publication Date: 2014-12-10
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been found that collagen used in medical biomaterials still has problems such as poor mechanical properties, poor biodegradability and low structural stability, which seriously restricts the application of collagen.

Method used

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  • High-purity natural collagenous fiber and preparation method thereof
  • High-purity natural collagenous fiber and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Pretreatment: Take fresh and traceable animal tendon or Achilles tendon, manually remove impurities such as muscle, marginal cartilage, blood vessels and fascia tissue on the surface of animal tendon or Achilles tendon, and then pour it into a grinder and grind it into 40 For the target granules, transfer animal tendon or Achilles tendon granules into a drum at room temperature, rinse them with normal saline and 75% ethanol for 30 minutes, and rinse them with double distilled water repeatedly;

[0040] (2) Water immersion: Take 100 parts by weight of animal tendon or Achilles tendon pellets and put them into the drum, add 100 parts by volume of distilled water into the drum at room temperature, add 0.5 parts by weight of Pingpingjia, turn for 10 minutes, and stop for 30 minutes. Then add 0.4 parts by weight of sodium carbonate, rotate intermittently, rotate for 10 minutes, stop for 30 minutes, repeat 3 times; then control the waste liquid, wash with running water for...

Embodiment 2

[0047] (1) Pretreatment: Take fresh and traceable animal tendon or Achilles tendon, manually remove impurities such as muscle, marginal cartilage, blood vessels and fascia tissue on the surface of animal tendon or Achilles tendon, and then pour it into a grinder to grind it into 6 0-mesh granules, transfer the animal tendon or Achilles tendon granules into the drum at room temperature, rinse with normal saline and 75% ethanol for 60 minutes, and then rinse with double distilled water repeatedly;

[0048] (2) Water immersion: Take 100 parts by weight of animal tendon or Achilles tendon granules and put them into the drum, add 150 parts by volume of distilled water into the drum at room temperature, add 1 part by weight of Pingpingjia, turn for 10 minutes, and stop for 30 minutes. Then add 1 part by weight of sodium carbonate, rotate intermittently, rotate for 10 minutes, stop for 30 minutes, repeat 4 times. Then dry the waste liquid, wash with running water for 15 minutes, and ...

Embodiment 3

[0055] (1) Pretreatment: Take fresh and traceable animal tendon or Achilles tendon, manually remove impurities such as muscle, marginal cartilage, blood vessels and fascia tissue on the surface of animal tendon or Achilles tendon, and then pour it into a grinder and crush it into 100 For the target granules, transfer animal tendon or Achilles tendon granules into a drum at room temperature, rinse with normal saline and 75% ethanol for 90 minutes, and then rinse with double distilled water repeatedly;

[0056] (2) Water immersion: Take 100 parts by weight of animal tendon or Achilles tendon granules and put them into the drum, add 300 parts by volume of distilled water into the drum at room temperature, add 2 parts by weight of Pingpingjia, turn for 10 minutes, and stop for 30 minutes. Then add 3 parts by weight of sodium carbonate, rotate intermittently, rotate for 10 minutes, stop for 30 minutes, repeat 5 times; then control the waste liquid, wash with running water for 20 min...

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Abstract

The invention discloses a high-purity natural collagenous fiber and a preparation method thereof. The collagenous fiber is characterized in that fresh traceable animal muscle tendon or achilles tendon is used as a raw material, and is subjected to pre-treatment, water soaking, supercritical carbon dioxide fluid degreasing, alkali leaching, first enzyme treatment, acid leaching, secondary enzyme treatment, salting out, centrifugal purification and other processes to obtain the high-purity natural collagenous fiber. The high-purity natural collagenous fiber prepared by the method is aggregation of natural collagen molecules, has the quarternary structure of collagen and the intrinsic bioactivity of collagen, and has more spatial structure features of collagen in natural tissues, so that the natural tissue structure can be further simulated. Compared with a collagen material, the natural collagenous fiber prepared by the method has the advantages of excellent mechanical performance, thermal stability and biodegradation performance, and the structure of the natural collagenous fiber is relatively stable and easy to store; and characteristic group expression of cells can be activated, and normal characteristic expression of cells can be maintained, so that adhesion, growth and proliferation of cells can be benefited. The natural collagenous fiber can be used for preparing hemostatic materials, tissue engineering scaffold materials, medical plastic and cosmetic materials and the like.

Description

technical field [0001] The invention relates to high-purity natural collagen fibers and a preparation method thereof, belonging to the field of biomedical material preparation. Background technique [0002] Collagen is the main structural protein that makes up connective tissue (such as skin, tendon, bone, etc.), accounting for about one-third of the total protein in mammals. Collagen, as the main extracellular matrix of organisms, plays a role in maintaining the shape of tissues, organs and skin and inducing the repair of various damaged tissues. Collagen has good biocompatibility, biodegradability, low toxicity, weak antigenicity, and can activate the expression of cell specific groups, which is beneficial to cell adhesion, growth, proliferation, and differentiation, so it is widely used in medical biomaterials Development and medical clinical treatment. It also plays a pivotal role in food, beauty cosmetics, health care products, papermaking and fermentation. However, ...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L27/24A61L31/04C12P21/06
CPCY02P20/54
Inventor 但卫华琚海燕但年华刘新华
Owner SICHUAN UNIV
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