Synthetic method and application of antibacterial peptide

A synthesis method and technology of antimicrobial peptides, applied in the field of synthesis of antimicrobial peptides, can solve the problems of long time required, side reactions, and complex procedures, and achieve the effects of low cost, simple production process, and short synthesis time

Active Publication Date: 2015-01-28
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The process is complicated, and side reactions are likely to occur in the process of cutting from the resin, and special...

Method used

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  • Synthetic method and application of antibacterial peptide
  • Synthetic method and application of antibacterial peptide
  • Synthetic method and application of antibacterial peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Lysine or arginine was mixed with leucine (manufactured by AMRESCO, L-type) in different proportions, and after thermal polymerization for 4 hours, leucine was added or no leucine was thermally polymerized and cross-linked for 1 hour. The antibacterial effect of each antimicrobial peptide on Escherichia coli DH5α and Staphylococcus aureus ATCC6538.

[0059] One, the synthetic method of antimicrobial peptide:

[0060] S1. Weigh lysine (or arginine) and leucine according to the mass ratio of 1:1, 2:1, 3:1, and 4:1, or only weigh an appropriate amount of lysine, and put them into In a 10 ml beaker, add sulfolane and phosphoric acid with a volume ratio of 16:1 into each beaker, the sulfolane and phosphoric acid are just submerged in the amino acid raw material, baked at 150°C for 4 hours and then taken out;

[0061] S2. Add appropriate amount of leucine to one part and bake at 150°C for 1 hour, and take out the other part without adding leucine and bake at 150°C for 1 hour...

Embodiment 2

[0070] Lysine or arginine was mixed with leucine (manufactured by AMRESCO, L-type) respectively in different proportions, and thermally polymerized for 6 hours. The antimicrobial effect of synthesized antimicrobial peptides on Escherichia coli MG1655, Staphylococcus aureus Y5, Smegmatis smegmatis, Pseudomonas aeruginosa, Alginolyticus, Aeromonas hydrophila and Pasteurella was studied.

[0071] One, the synthetic method of antimicrobial peptide:

[0072] S1. Weigh lysine (or arginine) and leucine according to the mass ratio of 1:1, 2:1, 3:1, and 4:1, or only weigh an appropriate amount of lysine, and put them into Add sulfolane and phosphoric acid at a volume ratio of 3:1 to each beaker in a 10 ml beaker, bake at 150°C for 6 hours, and then take it out.

[0073] S2. Wash three times with 3 times the volume of absolute ethanol, centrifuge at 6000 rpm for 10 minutes, dry in an oven at 60°C, and store in a refrigerator at 4°C for later use.

[0074] 2. Antibacterial peptide inhi...

Embodiment 3

[0079] One, the synthetic method of antimicrobial peptide:

[0080] S1. Weigh lysine (or arginine) and leucine (produced by AMRESCO, L type; domestic chemically pure) according to the mass ratio of 2:1, put them into 10 ml beakers respectively, and add volume to each beaker Sulfolane and phosphoric acid at a ratio of 4:1, baked at 160°C for 3 hours and then taken out; the above-mentioned amino acid raw materials are selected from domestic chemically pure or imported amino acids.

[0081] S2. Wash three times with 3 times the volume of absolute ethanol, centrifuge at 6000 rpm for 10 minutes, dry in the air, and store in a refrigerator at 4°C.

[0082] 2. Bacteriostatic zone test of antimicrobial peptides: The experimental steps of the bacteriostatic zone are the same as in Example 1, and the experimental results are shown in Table 4.

[0083] The bacteriostasis effect of the antibacterial peptide synthesized in embodiment 3 of table 4

[0084]

[0085] a means: domesticall...

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Abstract

The invention discloses a synthetic method and an application of an antibacterial peptide. The method comprises the following steps: S1, adding a solvent and phosphoric acid into a proper amount of an amino acid raw material and roasting for 2-6 hours at 150-180 DEG C, wherein the ratio of the solvent to phosphoric acid is (3-16) to 1; and S2, taking out a dried reactant and washing by using absolute ethyl alcohol, centrifuging and airing the reactant to obtain a product antibacterial peptide, wherein the solvent is sulfolane or vegetable oil; when the solvent is sulfolane, the amino acid raw material comprises lysine or/and arginine; when the solvent is vegetable oil, the amino acid raw material comprises lysine or arginine and one or more of leucine, isoleucine, valine, phenylalanine, alanine, aspartic acid, glutamic acid, glycine, serine or threonine are added. The amino acid raw material used by the method disclosed by the invention is just lysine or/and arginine, so that the method is simple and fast and economical in synthetic cost. The synthesized antibacterial peptide has high antibacterial activity to various bacteria and can be produced and applied on a large scale.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a synthesis method and application of an antibacterial peptide. Background technique [0002] Antibiotics were once used on a large scale and in high doses due to their excellent curative effect on infectious diseases. While greatly reducing human suffering, it also brought about the problem of bacterial drug resistance. From the discovery of penicillin-resistant Neisseria gonorrhoeae in 1960 to the drug resistance gene NDM-1 ravaging the world in 2009, the discovery of drug-resistant bacteria runs through the history of antibiotic use. The mechanism of action of antibiotics can be summarized as follows: inhibition of nucleic acid synthesis, inhibition of protein synthesis, change of cell membrane permeability, interference with cell wall formation, acting on energy metabolism system or as anti-metabolites. [0003] Antimicrobial peptides are a series of immune response products pro...

Claims

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Application Information

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IPC IPC(8): C07K1/00A61K38/02A61P31/04A61P31/10
CPCY02A50/30
Inventor 刘秋云何建国彭静立付敏邢梦王媛
Owner SUN YAT SEN UNIV
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