Method for extracting microbial oil

A technology for microbial oil and oil-producing microorganisms, which is applied in the production of fat and fat oil/fat, etc. It can solve the problems of increasing the cost of wastewater treatment and solvent recovery, increasing the difficulty of separation, and increasing the contact area, so as to avoid regulations and food safety risks. , The effect of reducing the difficulty of recycling and low acid value

Inactive Publication Date: 2015-04-01
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcoming of this method is: dried thalline is broken by common pulverizer, and its purpose is mainly to thalline that bulky thalline is pulverized into smaller thallus (volume average particle diameter is 2-3mm), thereby increases and solvent Contact area, improve yield
The disadvantage of this method is that the wet bacteria contain a large amount of water, so it is easy to activate the enzyme system during the crushing and extraction, so that part of the triglycerides are hydrolyzed during the treatment process, and the mono- and diglycerides produced are easy to produce chlorine in the subsequent refining process. Harmful substances such as propanol ester, and are prone to emulsification during the process, which greatly increases the difficulty of separation; at the same time, after a large amount of wastewater is mixed with solvents, it also greatly increases the cost of wastewater treatment and solvent recovery, and is not conducive to environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Using Mortierella alpina as the fermentation strain, follow the steps below:

[0020] 1) inoculating and fermenting Mortierella alpina;

[0021] 2) The fermented liquid obtained after the fermentation is finished is filtered by a plate-and-frame filter press, further pulverized and dried to obtain dried bacteria with a water content of 20%, wherein the maximum temperature during drying is controlled at 45°C;

[0022] 3) Mix 200g of dry cells with 400ml of hexane evenly, and then place them in a colloid mill for physical wall breaking until the volume average particle size of the cells is 559 μm;

[0023] 4) After separating the aforementioned bacterial cell mixture through a centrifuge, a mixed oil is obtained;

[0024] 5) Desolventizing the mixed oil to obtain microbial oil rich in arachidonic acid. After determination, the oil yield of this oil extraction method is 84.2wt%. Furthermore, the acid value of the oil is 1.32 mgKOH / g, the peroxide value is 4.3 meq / kg, th...

Embodiment 2

[0026] Using the B. trispora species as the fermentation strain, follow the following steps successively:

[0027] 1) Inoculate and ferment B. trispora;

[0028] 2) The fermented liquid obtained after the fermentation is finished is filtered by a plate-and-frame filter press, further pulverized and dried to obtain dried bacteria with a water content of 18%, wherein the maximum temperature during drying is controlled at 48°C;

[0029] 3) Mix 2kg of dry cells with 6L of butane evenly, and then place them in a shearing machine for physical wall breaking until the volume average particle size of the cells is 477 μm;

[0030] 4) After separating the aforementioned bacterial cell mixture through a centrifuge, a mixed oil is obtained;

[0031] 5) Desolventizing the mixed oil to obtain microbial oil containing β-carotene. After determination, the oil yield of this oil extraction method is 86.3wt%. Further, the acid value of the oil is 1.28 mgKOH / g, the peroxide value is 4.0 meq / kg,...

Embodiment 3

[0033] Using Mortierella chrysogenum species as the fermentation strain, follow the steps below:

[0034] 1) Inoculate and ferment Mortierella chrysogenum;

[0035] 2) The fermented liquid obtained after the fermentation is finished is filtered by a plate-and-frame filter press, further pulverized and dried to obtain dried bacteria with a water content of 15%, wherein the maximum temperature during drying is controlled at 50°C;

[0036] 3) Mix 5kg of dry cells with 20L of petroleum ether evenly and place them in a ball mill for physical wall breaking until the volume average particle size of the cells is 505 μm;

[0037] 4) After separating the aforementioned bacterial cell mixture through a centrifuge, a mixed oil is obtained;

[0038] 5) Desolventizing the mixed oil to obtain microbial oil containing gamma-linolenic acid. After determination, the oil yield of this oil extraction method is 87.4wt%. Further, the acid value of the oil is 1.20 mgKOH / g, the peroxide value is 3...

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Abstract

The invention provides a method for extracting microbial oil. The method comprises the following steps: oil-producing microorganisms are inoculated and fermented; a fermentation liquor obtained after fermentation is further smashed and dried through a separating system, and dry biomasses with the water content not higher than 20 percent is obtained; after even mixing, physical wall breaking is performed on the dry biomasses and an organic solvent for extraction until the volume average diameter of thallus particles are smaller than 600 mum; an oil mixture is obtained through solid-liquid separation to a mixed solution after physical wall breaking; the microbial oil is obtained through desolvation to the oil mixture. According to the method for extracting microbial oil, provided by the invention, adding enzyme for wall breaking is not required, risks, caused by enzyme residue, on aspects of laws and regulations and food security, can be avoided, more deterioration reactions of oil under high temperature can be reduced greatly, and energy consumption is reduced.

Description

technical field [0001] The invention relates to a method for extracting microbial oil. Background technique [0002] Polyunsaturated fatty acid (PUFA) generally refers to a straight-chain fatty acid with two or more double bonds and a carbon chain length of 18-22. According to the position of the carbon atom where the double bond is located, it is generally divided into omega-3 series unsaturated fatty acids and omega-6 series unsaturated fatty acids. For example, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) belong to the omega-3 series, while linoleic acid and arachidonic acid (ARA) belong to the omega-6 series. Polyunsaturated fatty acids (PUFA) have many important physiological activities. It can regulate the normal metabolism of blood lipids and lipoproteins in the human body, reduce the content of cholesterol in the blood, and prevent cardiovascular and cerebrovascular diseases. At the same time, it can be converted into prostate glands in the body. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10
CPCC11B1/10
Inventor 汪志明李翔宇周强易德伟
Owner CABIO BIOTECH WUHAN CO LTD
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