A method for efficient synthesis and extraction of purple sweet potato anthocyanins
A purple sweet potato anthocyanin and extraction method technology, applied in the field of bioengineering, can solve the problems of difficult dissolution of target components, large loss of active components, high energy consumption, etc., to achieve the effects of ensuring stability, improving the purity of finished products, and extending the industrial chain
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Embodiment 1
[0019] (1) Raw material pretreatment: take fresh, non-rotten purple sweet potato as raw material, wash and mechanically mash it until it is ready for use; (2) Purple sweet potato cell culture: the purple sweet potato pulp obtained in step (1) 0.7 mmol•L-1 Triton X-100 treatment for 10-20 minutes, 50-60Mpa high hydrostatic pressure treatment for 5 minutes, inoculation: Aspergillus niger 5%, Monascus 5%, Lactic acid bacteria 0.5%, Bacillus licheniformis 1% , Rhodotorula viscosus 5%, Phanerochaete chrysosporium 2%, Bacillus cereus 2%, Saccharomyces cerevisiae 0.8%, add L-phenylalanine 20mg / L, L-tyrosine 3mg / L, culture medium Formula: 0.3% sucrose, 1.0% peptone, 0.3% glucose, 1.0% corn steep liquor, 1.0% bean cake powder, 0.3% NaCl, KH 2 PO 4 0.2%, pH value 2.1, cultured at 25°C for 6 days, collected the culture medium, the above percentage concentration is the mass percentage concentration, and the added mass ratio of L-phenylalanine and L-tyrosine is 1:1; (3) Separation and c...
Embodiment 2
[0021] (1) Raw material pretreatment: take fresh, non-rotten purple sweet potato as raw material, wash and mechanically mash it until it is ready for use; (2) Purple sweet potato cell culture: the purple sweet potato pulp obtained in step (1) 0.7 mmol•L-1 Triton X-100 treatment for 10-20 minutes, 50-60Mpa high hydrostatic pressure treatment for 5 minutes, inoculation: Aspergillus niger 6%, Monascus 6%, Lactic acid bacteria 0.6%, Bacillus licheniformis 2% , Rhodotorula viscosus 6%, Phanerochaete chrysosporium 3%, Bacillus cereus 4%, Saccharomyces cerevisiae 1.0%, add L-phenylalanine 30 mg / L, L-tyrosine 5 mg / L, Medium formula: 0.4% sucrose, 1.2% peptone, 0.4% glucose, 2.0% corn steep liquor, 2.0% bean cake powder, 0.6% NaCl, KH 2 PO 4 0.4%, pH 3.5, cultivated at 28°C for 7 days, collected the culture medium, the above percentage concentration is the mass percentage concentration, and the mass ratio of L-phenylalanine to L-tyrosine is 1:1; (3) Separation and concentration of t...
Embodiment 3
[0023] (1) Raw material pretreatment: take fresh, non-rotten purple sweet potato as raw material, wash and mechanically mash it until it is ready for use; (2) Purple sweet potato cell culture: the purple sweet potato pulp obtained in step (1) 0.7 mmol•L-1 Triton X-100 treatment for 10-20 minutes, 50-60Mpa high hydrostatic pressure treatment for 5 minutes, inoculation: Aspergillus niger 5-7%, Monascus 7%, Lactic acid bacteria 0.8%, Bacillus licheniformis 3%, Rhodotorula viscosus 7%, Phanerochaete chrysosporium 5%, Bacillus cereus 5%, Saccharomyces cerevisiae 1.2%, add L-phenylalanine 40 mg / L, L-tyrosine 8 mg / L, medium formula: 0.6% sucrose, 2.0% peptone, 0.6% glucose, 3.0% corn steep liquor, 3.0% bean cake powder, 0.8% NaCl, KH 2 PO 4 0.6%, pH value 4.5, cultivated at 30°C for 8 days, collected the culture fluid, the above percentage concentration is the mass percentage concentration, and the mass ratio of L-phenylalanine to L-tyrosine is 1:1; (3) Separation and concentrati...
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