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A method for efficient synthesis and extraction of purple sweet potato anthocyanins

A purple sweet potato anthocyanin and extraction method technology, applied in the field of bioengineering, can solve the problems of difficult dissolution of target components, large loss of active components, high energy consumption, etc., to achieve the effects of ensuring stability, improving the purity of finished products, and extending the industrial chain

Active Publication Date: 2018-04-27
广东一家亲营养科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to aim at the defects that purple sweet potato anthocyanins are mainly concentrated in purple sweet potato cells, and a large amount of anthocyanin precursors are not fully synthesized and utilized, resulting in difficult dissolution of target components, large loss of active components, high energy consumption, and high cost , proposed a method for cultivating purple sweet potato cells to synthesize anthocyanins, and established a method for the synthesis and extraction of anthocyanins from purple sweet potato with high extraction rate, strong operability, low cost and mild conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Raw material pretreatment: take fresh, non-rotten purple sweet potato as raw material, wash and mechanically mash it until it is ready for use; (2) Purple sweet potato cell culture: the purple sweet potato pulp obtained in step (1) 0.7 mmol•L-1 Triton X-100 treatment for 10-20 minutes, 50-60Mpa high hydrostatic pressure treatment for 5 minutes, inoculation: Aspergillus niger 5%, Monascus 5%, Lactic acid bacteria 0.5%, Bacillus licheniformis 1% , Rhodotorula viscosus 5%, Phanerochaete chrysosporium 2%, Bacillus cereus 2%, Saccharomyces cerevisiae 0.8%, add L-phenylalanine 20mg / L, L-tyrosine 3mg / L, culture medium Formula: 0.3% sucrose, 1.0% peptone, 0.3% glucose, 1.0% corn steep liquor, 1.0% bean cake powder, 0.3% NaCl, KH 2 PO 4 0.2%, pH value 2.1, cultured at 25°C for 6 days, collected the culture medium, the above percentage concentration is the mass percentage concentration, and the added mass ratio of L-phenylalanine and L-tyrosine is 1:1; (3) Separation and c...

Embodiment 2

[0021] (1) Raw material pretreatment: take fresh, non-rotten purple sweet potato as raw material, wash and mechanically mash it until it is ready for use; (2) Purple sweet potato cell culture: the purple sweet potato pulp obtained in step (1) 0.7 mmol•L-1 Triton X-100 treatment for 10-20 minutes, 50-60Mpa high hydrostatic pressure treatment for 5 minutes, inoculation: Aspergillus niger 6%, Monascus 6%, Lactic acid bacteria 0.6%, Bacillus licheniformis 2% , Rhodotorula viscosus 6%, Phanerochaete chrysosporium 3%, Bacillus cereus 4%, Saccharomyces cerevisiae 1.0%, add L-phenylalanine 30 mg / L, L-tyrosine 5 mg / L, Medium formula: 0.4% sucrose, 1.2% peptone, 0.4% glucose, 2.0% corn steep liquor, 2.0% bean cake powder, 0.6% NaCl, KH 2 PO 4 0.4%, pH 3.5, cultivated at 28°C for 7 days, collected the culture medium, the above percentage concentration is the mass percentage concentration, and the mass ratio of L-phenylalanine to L-tyrosine is 1:1; (3) Separation and concentration of t...

Embodiment 3

[0023] (1) Raw material pretreatment: take fresh, non-rotten purple sweet potato as raw material, wash and mechanically mash it until it is ready for use; (2) Purple sweet potato cell culture: the purple sweet potato pulp obtained in step (1) 0.7 mmol•L-1 Triton X-100 treatment for 10-20 minutes, 50-60Mpa high hydrostatic pressure treatment for 5 minutes, inoculation: Aspergillus niger 5-7%, Monascus 7%, Lactic acid bacteria 0.8%, Bacillus licheniformis 3%, Rhodotorula viscosus 7%, Phanerochaete chrysosporium 5%, Bacillus cereus 5%, Saccharomyces cerevisiae 1.2%, add L-phenylalanine 40 mg / L, L-tyrosine 8 mg / L, medium formula: 0.6% sucrose, 2.0% peptone, 0.6% glucose, 3.0% corn steep liquor, 3.0% bean cake powder, 0.8% NaCl, KH 2 PO 4 0.6%, pH value 4.5, cultivated at 30°C for 8 days, collected the culture fluid, the above percentage concentration is the mass percentage concentration, and the mass ratio of L-phenylalanine to L-tyrosine is 1:1; (3) Separation and concentrati...

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Abstract

The invention discloses a high-efficiency method for synthesizing and extracting anthocyanins in purple sweet potato, in particular to a technique for synthesizing, extracting and preparing anthocyanins in purple sweet potato. Purple sweet potato cells were cultured by high hydrostatic pressure assisted microbial fermentation, and anthocyanins in purple sweet potato were extracted by ultrafiltration chromatography. Using high hydrostatic pressure to assist microbial metabolites to destroy the cell wall of purple sweet potato fruit, so that anthocyanins can be fully released; at the same time, adding L-phenylalanine and red yeast rhodotorula to promote the synthesis of anthocyanin precursors in purple sweet potato anthocyanin. After centrifugation, ultrafiltration separation, chromatographic purification, vacuum freeze-drying, etc., the fermentation broth is treated to obtain anthocyanins with a purity of 96.34%, which increases the extraction amount of anthocyanins by 15%-30%. The invention has the advantages of simple operation, clean and harmless production process, low cost, high anthocyanin yield and high purity, does not require microbial degradation in the human intestinal tract, has high medical and health care value, is suitable for large-scale production and application, and has great market prospects.

Description

technical field [0001] The invention relates to a method for efficiently synthesizing and extracting purple sweet potato anthocyanins, belonging to the technical field of bioengineering. Background technique [0002] Numerous studies have shown that dietary regulation and nutritional supplementation can improve human health and prevent disease. Reasonable nutritional therapy can improve the therapeutic effect of traditional medicine, reduce the amount of medicine used, and thus reduce the side effects of drug treatment. In recent years, studies have found that purple sweet potato is rich in a large amount of natural anthocyanins, which has antioxidant capacity, can improve human body function and prevent certain eye diseases. Therefore, how to efficiently extract and utilize anthocyanins in purple sweet potato has become an emerging frontier research topic in the 21st century. [0003] The anthocyanins obtained by the separation and extraction technology currently used: lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P17/06C07D311/62C12R1/685C12R1/10C12R1/085C12R1/865C12R1/645
Inventor 黄兴光黄自华
Owner 广东一家亲营养科技有限公司
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