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Novel triterpene compound in potentilla anserina, and preparation method and application thereof

A technology of triterpene compounds and compounds, applied in the direction of steroids, medical preparations containing active ingredients, organic chemistry, etc., can solve the problems that have not yet been reported on triterpene compounds

Inactive Publication Date: 2015-10-07
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Except that Hong Xia et al. isolated three pentacyclic triterpenoids from the rhizomes of Juemna (Hong Xia et al., Chinese Herbal Medicine, Vol. 37, No. 2, pp. 165-168), no other triterpenes in Juemia have been found Compound report

Method used

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  • Novel triterpene compound in potentilla anserina, and preparation method and application thereof
  • Novel triterpene compound in potentilla anserina, and preparation method and application thereof
  • Novel triterpene compound in potentilla anserina, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation, Preparation and Structure Confirmation of Compound I

[0030] (a) Crack the rhizome (10kg) and extract it with 75% ethanol under heat reflux (30L×3 times), combine the extracts, concentrate until there is no alcohol smell (6L), and then use petroleum ether (6L×3 times), acetic acid Ethyl ester (6L×3 times) and water-saturated n-butanol (6L×3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract (375g) and n-butanol extract respectively; (b) step (a ) in the ethyl acetate extract was removed with D101 macroporous resin, eluted with 15% ethanol (8L) and 70% ethanol (12L) in turn, collected 70% eluate, concentrated under reduced pressure to obtain 70% ethanol eluted concentration (125g); (c) The concentrate eluted with 70% ethanol in step (b) was separated with normal-phase silica gel, and dichloromethane with a volume ratio of 40:1, 20:1, 10:1 and 5:1 was used successively - Gradient elution with methanol yielded 4 component...

Embodiment 2

[0032] Embodiment 2: Compound I pharmacological action test

[0033] 1. Test method

[0034] 1. Cell culture

[0035] 1.1 Cell Recovery

[0036] The A375 melanoma cells frozen in the liquid nitrogen tank were taken out, quickly placed in warm water at 37°C, and gently shaken to melt as soon as possible (about 1 min). Then suck out the cell suspension, add it to the centrifuge tube that has been added with 2mL of medium, gently blow and mix, centrifuge at 800r / min for 5min, and discard the upper layer of medium. The cell suspension was prepared with 8 mL of DMEM medium containing 10% FBS and 1% Penicillium G / Streptomycin, and then inoculated into a 10 cm culture dish. Then placed in 5% CO 2 , and cultured in a 37°C constant temperature incubator.

[0037] 1.2 Cell passage

[0038] Observe under a microscope that the cell confluency reaches 80%-90% and can be passaged. Wash the cells twice with 2 mL of PBS, and then add 1 mL of 0.25% trypsin. Gently shake the culture pla...

Embodiment 3

[0084] Preparation of tablets: Compound I was first prepared according to the method in Example 1, and salts made from organic acids such as tartaric acid, or citric acid, formic acid, or oxalic acid, and inorganic acids such as hydrochloric acid, sulfuric acid, or phosphoric acid. The weight ratio of excipients is 1:7, adding excipients, granulating and compressing into tablets.

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Abstract

The invention discloses a novel triterpene compound in potentilla anserina, and a preparation method and application thereof, belonging to the technical field of medicines. The compound is reported for the first time, is a cycloartane-type triterpene compound with a novel structure and can be extracted from rhizome of potentilla anserina and then prepared through separation and purification. In-vitro test results show that the compound can inhibit propagation of melanoma cells, so the compound can be developed into a drug used for treating melanoma.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a cycloaltin-type triterpene compound isolated from Bracken japonicus, a pharmaceutical composition containing it, a preparation method and application thereof. Background technique [0002] The medicine Bracken is a plant of the Rosaceae Potentilla genus Bracken (Potentilla (Potentilla) Potentilla anserine The root of L.) has the effects of promoting body fluid and quenching thirst, invigorating the spleen and stomach, nourishing qi and nourishing blood, astringent and hemostasis, stopping diarrhea, relieving cough, and promoting phlegm. embolism. Bracken is rich in starch, fatty acids and 18 kinds of amino acids and multivitamins needed by the human body, and has high medical and nutritional value. This medicine is all recorded in books such as "Dictionary of Chinese Medicine" and "Chinese Herbal Medicine Commonly Used in Tibet". This product is very rich in w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K31/58A61P35/00
CPCC07J63/004
Inventor 赵晓辉岳会兰韩发
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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