A strain of Bacillus megaterium and its application

A technology of Bacillus megaterium and strains, applied in the field of biological control preparations, can solve the problems of long period of disease-resistant varieties, single resistance, pesticide residues, etc., and achieve the effects of good application prospects, fast growth rate, and large spore production.

Active Publication Date: 2018-02-23
SHANGDONG TENOV PESTICIDES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease-resistant variety has a long cycle and a single resistance
The use of chemicals for chemical control takes a long time and a large amount of use, resulting in pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in decreased control efficiency or even failure

Method used

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  • A strain of Bacillus megaterium and its application
  • A strain of Bacillus megaterium and its application
  • A strain of Bacillus megaterium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and purification of Bacillus megaterium strain STY006

[0028] Bacillus megaterium bacterial strain STY006 of the present invention adopts dilution plate method and plate streaking method to separate and obtain from soil, and separation method is:

[0029] ⑴Isolation of Bacillus: Soil sample collection, select plots planted with different types of crops in different regions of the country, use 5-point sampling method, collect appropriate amount of soil around and in the center of the plot within a depth of 10-20cm, and the same amount Mix well. Indicate the location, time and person of the collection. Weigh 1g of soil sample into 100mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of differe...

Embodiment 2

[0040] 1. Fermentation process of Bacillus megaterium strain STY006

[0041] LB liquid medium: formula: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0042] A large amount of solid fermentation medium formula (mass percentage): solid material: corn flour 65%, bran 35%; inorganic salt solution: potassium dihydrogen phosphate 3.5%, magnesium sulfate 0.04%, ammonium sulfate 4%, the remainder is water . The solid-to-liquid ratio is 1:1.8 (mass ratio).

[0043] Mass solid fermentation process of Bacillus megaterium strain STY006:

[0044] ①Cultivation of strains in seed solution: Pick a small amount of bacteria from the slant of the test tube of Bacillus megaterium strain STY006, transfer them to LB liquid medium, and cultivate them on a shaker at 30°C for 3 to 5 days. This is the seed solution;

[0045] ②Cultivation of solid production strains: Inoculate the seed liquid into solid medium (500mL Erlenmeyer flask) at a rati...

Embodiment 3

[0047] Embodiment 3 field efficacy test

[0048] This example provides relevant experiments on the control effect of the raw powder of Bacillus megaterium strain STY006 on cotton wilt.

[0049] 1) Test drug

[0050] Original powder of Bacillus megaterium strain STY006 (prepared in Example 2); 70% thiophanate-methyl wettable powder (commercially available).

[0051] 2) Test crops and control objects:

[0052] The test crop is cotton; the variety is Zhongmian No. 17;

[0053] Control object: Fusarium wilt.

[0054] 3) Test site conditions, test design and arrangement

[0055] The test site is located in the cotton field of Xinhu Village, Wanglao Township, Dongying City, Shandong Province. The soil is clay soil, and the occurrence of Fusarium wilt was severe in previous years. The cultivation conditions in the experimental field were uniform.

[0056] In this experiment, the ratio of the original powder of Bacillus megaterium STY006 to drug species was 1:200, 1:400, 1:600; ...

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Abstract

The invention discloses a strain of bacillus megaterium and its application. The strain is Bacillus megaterium STY006, and the preservation number is CGMCC No.11053. The invention also discloses a biological control preparation comprising the Bacillus megaterium strain STY006 and a preparation method thereof. Experiments have shown that the Bacillus megaterium strain of the present invention has the characteristics of fast growth, large spore production, wide action spectrum, strong stress resistance, rapid and large-scale colonization in the rhizosphere of plants, etc., so it has good application prospects. The biological control preparation prepared by the Bacillus megaterium strain can be used as a biological pesticide or biological fertilizer to prevent and control a variety of soil-borne plant bacterial diseases, including cotton fusarium wilt, Chinese cabbage soft rot, ginger blast, and walnut bacterial brown spot illness etc.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a bacillus capable of efficiently preventing and controlling plant diseases and its use, and a biological control agent prepared by using the bacillus. Background technique [0002] Plant diseases seriously affect the growth of agricultural and forestry crops, and even cause crop death, resulting in significant economic losses. For the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. The disease-resistant variety has a long cycle and a single resistance. Chemical control uses a long time and a large amount of pesticides, resulting in pesticide residues and environmental pollution. At the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in reduced control eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12R1/11
Inventor 戴宝牛赡光刘幸红王清海卢德鹏朱福庆王栋梁马瑞滨孔雪华李秀香
Owner SHANGDONG TENOV PESTICIDES
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