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Blue-light-emitting up-conversion material and preparation and application thereof

A conversion material, blue technology, applied in the direction of analysis materials, measuring devices, instruments, etc., can solve the problem of not detecting sulfaquinoxaline, etc., to reduce the influence of background mechanism, easy and fast operation, good biocompatibility Effect

Inactive Publication Date: 2016-06-08
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not been widely used to detect sulfaquinoxaline in food

Method used

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  • Blue-light-emitting up-conversion material and preparation and application thereof
  • Blue-light-emitting up-conversion material and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of water-soluble up-conversion fluorescent labeling material

[0028] Prepare up-conversion nanomaterials by thermal decomposition, the specific method is as follows: yttrium acetate C 6 h 17 o 10 Y, ytterbium acetate C 6 h 9 o 6 Yb.4H 2 O, thulium acetate C 6 h 9 Tm.xH 2 O was dissolved in a 100ml three-necked flask containing 6ml oleic acid (OA) and 17ml octadecene in a ratio of 79:19:2, heated to 100°C for 10 minutes in vacuum, then heated to 160°C for 30 minutes, Let cool to room temperature. Contains NaOH (0.1g) and NH 4 The methanol solution of F (0.148g) was added into a three-necked flask, and the mixed solution was heated to 80° C. and dried under vacuum for half an hour. Under the protection of nitrogen, the mixed solution was heated to 300° C. to reflux for one hour and then cooled to room temperature. The organic phase solution was centrifuged and washed three times with ethanol to obtain oil-soluble upconversion nanoparticl...

Embodiment 2

[0029] The carboxyl modification of the surface of OA-UCNPs by polyacrylic acid (PAA) belongs to the ligand exchange method. The oleic acid ligands on the surface of OA-UCNPs were exchanged for polyacrylic acid ligands to obtain water-soluble PAA-UCNPs. The specific method is as follows: Accurately weigh 0.5g of PAA, add 10mL of diethylene glycol (DEG) and mix evenly, heat to 110°C under nitrogen protection and stir vigorously. Accurately weigh 30 mg of OA-UCNPs, add 2 mL of toluene solution and mix well. The above solutions were mixed and heated to 230 °C for 1 h. Cool to room temperature, add excess dilute hydrochloric acid aqueous solution with a pH of 4-5, centrifuge, and discard the supernatant. Wash and centrifuge three times with pure water, dry, and store in a desiccator for later use. Example 2 Preparation of Immunosensing Probes and Fluorescent Signaling Probes

[0030]The sulfaquinoxaline monoclonal antibody was connected to the water-soluble up-converting mater...

Embodiment 3

[0032] Embodiment 3 Establishment of fluorescent immunoassay method

[0033] The present invention establishes a competitive immunoassay method based on antigen-antibody immune reaction combined with up-conversion fluorescent labeling and separation of polystyrene magnetic microspheres by optimizing the addition of immunosensing probes and fluorescent signal probes, with a detection limit of 0.1 ngmL -1 ,Such as figure 2 shown. Specific method: Take several 1.5ml small test tubes, add 100 μL immunomagnetic induction probe and 100 μL immunofluorescence signal probe respectively. Prepare a series of concentration gradient sulfaquinoxaline standard substances (0, 0.1, 1, 10, 100ngmL -1 ) solution, and then add free sulfaquinoxaline standard solution in each small test tube successively. Reaction at room temperature for 1h. After the reaction, the combined products were separated under the action of an external magnetic field and washed three times with PBS. After reconstitu...

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Abstract

The invention provides a blue-light-emitting up-conversion material. Yttrium acetate C6H17O10Y, ytterbium acetate C6H9O6Yb.4H2O and thulium acetate C6H9Tm.xH2O serve as main raw materials for preparation. The blue-light-emitting up-conversion material is mainly characterized in that up-conversion fluorescence nano-particles NaYF4:Yb,Tm (the excitation wavelength is 980 nm and the emission wavelength is 477 nm) mark a sulfaquinoxaline monoclonal antibody and serve as a signal probe, polystyrene magnetic microspheres connected with a sulfaquinoxaline coating antigen serve as an induction probe, and thus a competitive immunoassay based on up-conversion particle marking and magnetic nano-particle quick separation is structured to detect sulfaquinoxaline in food, and the detection limit is 0.1 ng mL-1. The blue-light-emitting up-conversion material has the following advantages that detection steps are simple, separation is achieved through the action of an additionally-added magnetic field, operation steps are greatly simplified, and the detection time is shortened; sensitivity is good; sample pretreatment is easy, fast and environmentally friendly.

Description

technical field [0001] The invention belongs to the fields of immunology, physics, material chemistry and analytical chemistry, and in particular relates to a blue luminescent up-conversion material and its preparation and application. Background technique [0002] Sulfonamides (SAs) are a class of broad-spectrum antibacterial drugs, which are cheap and easy to use, and are widely used in the research fields of human disease treatment, veterinary clinic and animal feed additives. However, in recent years, due to the indiscriminate use and abuse of sulfa drugs, the phenomenon of excessive residues in animal foods has become more and more serious. After eating these foods, the human body may produce various toxic and side effects, and even cause organic damage. Its toxic effect is mainly manifested in carcinogenesis, causing allergic reactions, inhibiting the hematopoietic system, causing bacteria to develop drug resistance, and eventually losing the effect of treating human d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/577
CPCG01N33/533G01N33/577
Inventor 王硕生威胡高爽张燕王俊平
Owner TIANJIN UNIV OF SCI & TECH
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